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Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats

OBJECTIVE: Muscle injuries are common, and their treatment requires costs and time off. Platelet rich plasma and low level laser therapy have been shown to be affordable and easy to use. The aim of this study was to evaluate the associated effects of low level laser therapy and platelet rich plasma...

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Autores principales: Garcia, Thiago Alves, Camargo, Regina Celi Trindade, Koike, Tatiana Emy, Ozaki, Guilherme Akio Tamura, Castoldi, Robson Chacon, Camargo Filho, José Carlos Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Departamento de Fisioterapia da Universidade Federal de Sao Carlos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693427/
https://www.ncbi.nlm.nih.gov/pubmed/28733091
http://dx.doi.org/10.1016/j.bjpt.2017.06.007
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author Garcia, Thiago Alves
Camargo, Regina Celi Trindade
Koike, Tatiana Emy
Ozaki, Guilherme Akio Tamura
Castoldi, Robson Chacon
Camargo Filho, José Carlos Silva
author_facet Garcia, Thiago Alves
Camargo, Regina Celi Trindade
Koike, Tatiana Emy
Ozaki, Guilherme Akio Tamura
Castoldi, Robson Chacon
Camargo Filho, José Carlos Silva
author_sort Garcia, Thiago Alves
collection PubMed
description OBJECTIVE: Muscle injuries are common, and their treatment requires costs and time off. Platelet rich plasma and low level laser therapy have been shown to be affordable and easy to use. The aim of this study was to evaluate the associated effects of low level laser therapy and platelet rich plasma on the treatment of the soleus muscle injured by strain in rats. METHODS: Thirty-five rats were randomly allocated into five groups: Control (C), Injury Control (IC), injury PRP (IP), injury LLLT (ILT) and injury LLLT and PRP (ILTP). The strain injury was induced in the soleus muscle and the IP group received application of platelet rich plasma immediately after the lesion, while the ILT group received low level laser therapy. After seven days, all animals were euthanized and the soleus muscle removed for further histological analysis. RESULTS: The association of both treatments (ILTP) resulted in better histological aspects than the low level laser therapy and platelet rich plasma alone, when compared with the injury group (IC). The collagen analysis exhibited a significant increase in the ILT group (2.99 SD = 1.13) compared to the C (1.88 SD = 0.41, p = 0.012) and IP (2.04 SD = 0.44, p = 0.018). CONCLUSION: The association of low level laser therapy with platelet rich plasma produced better results on muscle injury compared to the isolated use of these therapies. Furthermore, none of the treatments could modulate the collagen deposition in relation to injury group.
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spelling pubmed-56934272017-11-21 Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats Garcia, Thiago Alves Camargo, Regina Celi Trindade Koike, Tatiana Emy Ozaki, Guilherme Akio Tamura Castoldi, Robson Chacon Camargo Filho, José Carlos Silva Braz J Phys Ther Original Research OBJECTIVE: Muscle injuries are common, and their treatment requires costs and time off. Platelet rich plasma and low level laser therapy have been shown to be affordable and easy to use. The aim of this study was to evaluate the associated effects of low level laser therapy and platelet rich plasma on the treatment of the soleus muscle injured by strain in rats. METHODS: Thirty-five rats were randomly allocated into five groups: Control (C), Injury Control (IC), injury PRP (IP), injury LLLT (ILT) and injury LLLT and PRP (ILTP). The strain injury was induced in the soleus muscle and the IP group received application of platelet rich plasma immediately after the lesion, while the ILT group received low level laser therapy. After seven days, all animals were euthanized and the soleus muscle removed for further histological analysis. RESULTS: The association of both treatments (ILTP) resulted in better histological aspects than the low level laser therapy and platelet rich plasma alone, when compared with the injury group (IC). The collagen analysis exhibited a significant increase in the ILT group (2.99 SD = 1.13) compared to the C (1.88 SD = 0.41, p = 0.012) and IP (2.04 SD = 0.44, p = 0.018). CONCLUSION: The association of low level laser therapy with platelet rich plasma produced better results on muscle injury compared to the isolated use of these therapies. Furthermore, none of the treatments could modulate the collagen deposition in relation to injury group. Departamento de Fisioterapia da Universidade Federal de Sao Carlos 2017 2017-07-04 /pmc/articles/PMC5693427/ /pubmed/28733091 http://dx.doi.org/10.1016/j.bjpt.2017.06.007 Text en © 2017 Associaç˜ao Brasileira de Pesquisa e Pós-Graduaç˜ao em Fisioterapia. Published by Elsevier Editora Ltda. All rights reserved.
spellingShingle Original Research
Garcia, Thiago Alves
Camargo, Regina Celi Trindade
Koike, Tatiana Emy
Ozaki, Guilherme Akio Tamura
Castoldi, Robson Chacon
Camargo Filho, José Carlos Silva
Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats
title Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats
title_full Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats
title_fullStr Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats
title_full_unstemmed Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats
title_short Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats
title_sort histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693427/
https://www.ncbi.nlm.nih.gov/pubmed/28733091
http://dx.doi.org/10.1016/j.bjpt.2017.06.007
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