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Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae

The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, is the most serious insect pest of corn (Zea mays L.) in the United States and parts of Europe, and arguably one of the world’s most expensive pests to control. Several diet formulations are currently used by industry and publi...

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Autores principales: Huynh, Man P., Meihls, Lisa N., Hibbard, Bruce E., Lapointe, Stephen L., Niedz, Randall P., Ludwick, Dalton C., Coudron, Thomas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693442/
https://www.ncbi.nlm.nih.gov/pubmed/29149192
http://dx.doi.org/10.1371/journal.pone.0187997
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author Huynh, Man P.
Meihls, Lisa N.
Hibbard, Bruce E.
Lapointe, Stephen L.
Niedz, Randall P.
Ludwick, Dalton C.
Coudron, Thomas A.
author_facet Huynh, Man P.
Meihls, Lisa N.
Hibbard, Bruce E.
Lapointe, Stephen L.
Niedz, Randall P.
Ludwick, Dalton C.
Coudron, Thomas A.
author_sort Huynh, Man P.
collection PubMed
description The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, is the most serious insect pest of corn (Zea mays L.) in the United States and parts of Europe, and arguably one of the world’s most expensive pests to control. Several diet formulations are currently used by industry and public researchers to evaluate WCR larvae in diet-toxicity bioassays. However, a publicly available diet that produces normative insects that are physiologically similar to WCR larvae reared on corn roots will accelerate development of management technologies. We report a new diet formulation that supports improved weight gain, larval development and survival compared with the only public diet for WCR that is currently available in the refereed literature. The formulation was created by using response surface methods combined with n-dimensional mixture designs to identify and improve the formulation of key ingredients. Weight gain increased two-fold, and survival and molting rates increased from 93% and 90%, respectively when reared on the public diet, to approximately 99% for both survival and molting at 11 days when reared on our new formulation. This new formulation provides a standardized growth medium for WCR larvae that will facilitate comparison of research results from various working groups and compliance with regulatory requirements.
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spelling pubmed-56934422017-11-30 Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae Huynh, Man P. Meihls, Lisa N. Hibbard, Bruce E. Lapointe, Stephen L. Niedz, Randall P. Ludwick, Dalton C. Coudron, Thomas A. PLoS One Research Article The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, is the most serious insect pest of corn (Zea mays L.) in the United States and parts of Europe, and arguably one of the world’s most expensive pests to control. Several diet formulations are currently used by industry and public researchers to evaluate WCR larvae in diet-toxicity bioassays. However, a publicly available diet that produces normative insects that are physiologically similar to WCR larvae reared on corn roots will accelerate development of management technologies. We report a new diet formulation that supports improved weight gain, larval development and survival compared with the only public diet for WCR that is currently available in the refereed literature. The formulation was created by using response surface methods combined with n-dimensional mixture designs to identify and improve the formulation of key ingredients. Weight gain increased two-fold, and survival and molting rates increased from 93% and 90%, respectively when reared on the public diet, to approximately 99% for both survival and molting at 11 days when reared on our new formulation. This new formulation provides a standardized growth medium for WCR larvae that will facilitate comparison of research results from various working groups and compliance with regulatory requirements. Public Library of Science 2017-11-17 /pmc/articles/PMC5693442/ /pubmed/29149192 http://dx.doi.org/10.1371/journal.pone.0187997 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Huynh, Man P.
Meihls, Lisa N.
Hibbard, Bruce E.
Lapointe, Stephen L.
Niedz, Randall P.
Ludwick, Dalton C.
Coudron, Thomas A.
Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae
title Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae
title_full Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae
title_fullStr Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae
title_full_unstemmed Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae
title_short Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae
title_sort diet improvement for western corn rootworm (coleoptera: chrysomelidae) larvae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693442/
https://www.ncbi.nlm.nih.gov/pubmed/29149192
http://dx.doi.org/10.1371/journal.pone.0187997
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