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Metabolic dynamics restricted by conserved carriers: Jamming and feedback

To uncover the processes and mechanisms of cellular physiology, it first necessary to gain an understanding of the underlying metabolic dynamics. Recent studies using a constraint-based approach succeeded in predicting the steady states of cellular metabolic systems by utilizing conserved quantities...

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Detalles Bibliográficos
Autores principales: Hatakeyama, Tetsuhiro S., Furusawa, Chikara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693451/
https://www.ncbi.nlm.nih.gov/pubmed/29112954
http://dx.doi.org/10.1371/journal.pcbi.1005847
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author Hatakeyama, Tetsuhiro S.
Furusawa, Chikara
author_facet Hatakeyama, Tetsuhiro S.
Furusawa, Chikara
author_sort Hatakeyama, Tetsuhiro S.
collection PubMed
description To uncover the processes and mechanisms of cellular physiology, it first necessary to gain an understanding of the underlying metabolic dynamics. Recent studies using a constraint-based approach succeeded in predicting the steady states of cellular metabolic systems by utilizing conserved quantities in the metabolic networks such as carriers such as ATP/ADP as an energy carrier or NADH/NAD(+) as a hydrogen carrier. Although such conservation quantities restrict not only the steady state but also the dynamics themselves, the latter aspect has not yet been completely understood. Here, to study the dynamics of metabolic systems, we propose adopting a carrier cycling cascade (CCC), which includes the dynamics of both substrates and carriers, a commonly observed motif in metabolic systems such as the glycolytic and fermentation pathways. We demonstrate that the conservation laws lead to the jamming of the flux and feedback. The CCC can show slow relaxation, with a longer timescale than that of elementary reactions, and is accompanied by both robustness against small environmental fluctuations and responsiveness against large environmental changes. Moreover, the CCC demonstrates robustness against internal fluctuations due to the feedback based on the moiety conservation. We identified the key parameters underlying the robustness of this model against external and internal fluctuations and estimated it in several metabolic systems.
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spelling pubmed-56934512017-11-30 Metabolic dynamics restricted by conserved carriers: Jamming and feedback Hatakeyama, Tetsuhiro S. Furusawa, Chikara PLoS Comput Biol Research Article To uncover the processes and mechanisms of cellular physiology, it first necessary to gain an understanding of the underlying metabolic dynamics. Recent studies using a constraint-based approach succeeded in predicting the steady states of cellular metabolic systems by utilizing conserved quantities in the metabolic networks such as carriers such as ATP/ADP as an energy carrier or NADH/NAD(+) as a hydrogen carrier. Although such conservation quantities restrict not only the steady state but also the dynamics themselves, the latter aspect has not yet been completely understood. Here, to study the dynamics of metabolic systems, we propose adopting a carrier cycling cascade (CCC), which includes the dynamics of both substrates and carriers, a commonly observed motif in metabolic systems such as the glycolytic and fermentation pathways. We demonstrate that the conservation laws lead to the jamming of the flux and feedback. The CCC can show slow relaxation, with a longer timescale than that of elementary reactions, and is accompanied by both robustness against small environmental fluctuations and responsiveness against large environmental changes. Moreover, the CCC demonstrates robustness against internal fluctuations due to the feedback based on the moiety conservation. We identified the key parameters underlying the robustness of this model against external and internal fluctuations and estimated it in several metabolic systems. Public Library of Science 2017-11-07 /pmc/articles/PMC5693451/ /pubmed/29112954 http://dx.doi.org/10.1371/journal.pcbi.1005847 Text en © 2017 Hatakeyama, Furusawa http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hatakeyama, Tetsuhiro S.
Furusawa, Chikara
Metabolic dynamics restricted by conserved carriers: Jamming and feedback
title Metabolic dynamics restricted by conserved carriers: Jamming and feedback
title_full Metabolic dynamics restricted by conserved carriers: Jamming and feedback
title_fullStr Metabolic dynamics restricted by conserved carriers: Jamming and feedback
title_full_unstemmed Metabolic dynamics restricted by conserved carriers: Jamming and feedback
title_short Metabolic dynamics restricted by conserved carriers: Jamming and feedback
title_sort metabolic dynamics restricted by conserved carriers: jamming and feedback
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693451/
https://www.ncbi.nlm.nih.gov/pubmed/29112954
http://dx.doi.org/10.1371/journal.pcbi.1005847
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