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Epidemiology and clinical profile of pathogens responsible for the hospitalization of children in Sousse area, Tunisia
This study aimed to identify a broad spectrum of respiratory pathogens from hospitalized and not-preselected children with acute respiratory tract infections in the Farhat Hached University-hospital of Sousse, Tunisia. Between September 2013 and December 2014, samples from 372 children aged between...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693464/ https://www.ncbi.nlm.nih.gov/pubmed/29149199 http://dx.doi.org/10.1371/journal.pone.0188325 |
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author | Brini, Ines Guerrero, Aida Hannachi, Naila Bouguila, Jihene Orth-Höller, Dorothea Bouhlel, Amira Boughamoura, Lamia Hetzer, Benjamin Borena, Wegene Schiela, Britta Von Laer, Dorothee Boukadida, Jalel Stoiber, Heribert |
author_facet | Brini, Ines Guerrero, Aida Hannachi, Naila Bouguila, Jihene Orth-Höller, Dorothea Bouhlel, Amira Boughamoura, Lamia Hetzer, Benjamin Borena, Wegene Schiela, Britta Von Laer, Dorothee Boukadida, Jalel Stoiber, Heribert |
author_sort | Brini, Ines |
collection | PubMed |
description | This study aimed to identify a broad spectrum of respiratory pathogens from hospitalized and not-preselected children with acute respiratory tract infections in the Farhat Hached University-hospital of Sousse, Tunisia. Between September 2013 and December 2014, samples from 372 children aged between 1 month and 5 years were collected, and tested using multiplex real-time RT-PCR by a commercial assay for 21 respiratory pathogens. In addition, samples were screened for the presence of Streptococcus pneumoniae 16S rDNA using real-time PCR. The viral distribution and its association with clinical symptoms were statistically analyzed. Viral pathogens were detected in 342 (91.93%) of the samples of which 28.76% were single positive and 63.17% had multiple infections. The most frequent detected viruses were rhinovirus (55.64%), respiratory syncytial virus A/B (33.06%), adenovirus (25.00%), coronavirus NL63, HKU1, OC43, and 229E (21.50%), and metapneumovirus A/B (16.12%). Children in the youngest age group (1–3 months) exhibited the highest frequencies of infection. Related to their frequency of detection, RSV A/B was the most associated pathogen with patient’s demographic situation and clinical manifestations (p<0.05). Parainfluenza virus 1–4 and parechovirus were found to increase the risk of death (p<0.05). Adenovirus was statistically associated to the manifestation of gastroenteritis (p = 0.004). Rhinovirus infection increases the duration of oxygen support (p = 0.042). Coronavirus group was statistically associated with the manifestation of bronchiolitis (p = 0.009) and laryngitis (p = 0.017). Streptococcus pneumoniae DNA was detected in 143 (38.44%) of tested samples. However, only 53 samples had a concentration of C-reactive protein from equal to higher than 20 milligrams per liter, and 6 of them were single positive for Streptocuccus pneumoniae. This study confirms the high incidence of respiratory viruses in children hospitalized for acute respiratory tract infections in the Sousse area, Tunisia. |
format | Online Article Text |
id | pubmed-5693464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56934642017-11-30 Epidemiology and clinical profile of pathogens responsible for the hospitalization of children in Sousse area, Tunisia Brini, Ines Guerrero, Aida Hannachi, Naila Bouguila, Jihene Orth-Höller, Dorothea Bouhlel, Amira Boughamoura, Lamia Hetzer, Benjamin Borena, Wegene Schiela, Britta Von Laer, Dorothee Boukadida, Jalel Stoiber, Heribert PLoS One Research Article This study aimed to identify a broad spectrum of respiratory pathogens from hospitalized and not-preselected children with acute respiratory tract infections in the Farhat Hached University-hospital of Sousse, Tunisia. Between September 2013 and December 2014, samples from 372 children aged between 1 month and 5 years were collected, and tested using multiplex real-time RT-PCR by a commercial assay for 21 respiratory pathogens. In addition, samples were screened for the presence of Streptococcus pneumoniae 16S rDNA using real-time PCR. The viral distribution and its association with clinical symptoms were statistically analyzed. Viral pathogens were detected in 342 (91.93%) of the samples of which 28.76% were single positive and 63.17% had multiple infections. The most frequent detected viruses were rhinovirus (55.64%), respiratory syncytial virus A/B (33.06%), adenovirus (25.00%), coronavirus NL63, HKU1, OC43, and 229E (21.50%), and metapneumovirus A/B (16.12%). Children in the youngest age group (1–3 months) exhibited the highest frequencies of infection. Related to their frequency of detection, RSV A/B was the most associated pathogen with patient’s demographic situation and clinical manifestations (p<0.05). Parainfluenza virus 1–4 and parechovirus were found to increase the risk of death (p<0.05). Adenovirus was statistically associated to the manifestation of gastroenteritis (p = 0.004). Rhinovirus infection increases the duration of oxygen support (p = 0.042). Coronavirus group was statistically associated with the manifestation of bronchiolitis (p = 0.009) and laryngitis (p = 0.017). Streptococcus pneumoniae DNA was detected in 143 (38.44%) of tested samples. However, only 53 samples had a concentration of C-reactive protein from equal to higher than 20 milligrams per liter, and 6 of them were single positive for Streptocuccus pneumoniae. This study confirms the high incidence of respiratory viruses in children hospitalized for acute respiratory tract infections in the Sousse area, Tunisia. Public Library of Science 2017-11-17 /pmc/articles/PMC5693464/ /pubmed/29149199 http://dx.doi.org/10.1371/journal.pone.0188325 Text en © 2017 Brini et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Brini, Ines Guerrero, Aida Hannachi, Naila Bouguila, Jihene Orth-Höller, Dorothea Bouhlel, Amira Boughamoura, Lamia Hetzer, Benjamin Borena, Wegene Schiela, Britta Von Laer, Dorothee Boukadida, Jalel Stoiber, Heribert Epidemiology and clinical profile of pathogens responsible for the hospitalization of children in Sousse area, Tunisia |
title | Epidemiology and clinical profile of pathogens responsible for the hospitalization of children in Sousse area, Tunisia |
title_full | Epidemiology and clinical profile of pathogens responsible for the hospitalization of children in Sousse area, Tunisia |
title_fullStr | Epidemiology and clinical profile of pathogens responsible for the hospitalization of children in Sousse area, Tunisia |
title_full_unstemmed | Epidemiology and clinical profile of pathogens responsible for the hospitalization of children in Sousse area, Tunisia |
title_short | Epidemiology and clinical profile of pathogens responsible for the hospitalization of children in Sousse area, Tunisia |
title_sort | epidemiology and clinical profile of pathogens responsible for the hospitalization of children in sousse area, tunisia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693464/ https://www.ncbi.nlm.nih.gov/pubmed/29149199 http://dx.doi.org/10.1371/journal.pone.0188325 |
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