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Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment
BACKGROUND: The identification of microorganisms with excellent flocculant-producing capability and optimization of the fermentation process are necessary for the wide-scale application of bioflocculants. Thus, we evaluated the flocculant-producing ability of a novel strain identified by the screeni...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693566/ https://www.ncbi.nlm.nih.gov/pubmed/29149843 http://dx.doi.org/10.1186/s12896-017-0404-z |
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author | Chen, Zhen Li, Zhipeng Liu, Peize Liu, Yu Wang, Yuanpeng Li, Qingbiao He, Ning |
author_facet | Chen, Zhen Li, Zhipeng Liu, Peize Liu, Yu Wang, Yuanpeng Li, Qingbiao He, Ning |
author_sort | Chen, Zhen |
collection | PubMed |
description | BACKGROUND: The identification of microorganisms with excellent flocculant-producing capability and optimization of the fermentation process are necessary for the wide-scale application of bioflocculants. Thus, we evaluated the flocculant-producing ability of a novel strain identified by the screening of marine bacteria, and we report for the first time the properties of the bioflocculant produced by Alteromonas sp. in the treatment of dye wastewater. RESULTS: A bioflocculant-producing bacterium was isolated from seawater and identified as Alteromonas sp. CGMCC 10612. The optimal carbon and nitrogen sources for the strain were 30 g/L glucose and 1.5 g/L wheat flour. In a 2-L fermenter, the flocculating activity and bioflocculant yield reached maximum values of 2575.4 U/mL and 11.18 g/L, respectively. The bioflocculant was separated and showed good heat and pH stability. The purified bioflocculant was a proteoglycan consisting of 69.61% carbohydrate and 21.56% protein (wt/wt). Infrared spectrometry further indicated the presence of hydroxyl, carboxyl and amino groups preferred for flocculation. The bioflocculant was a nanoparticle polymer with an average mass of 394,000 Da. The purified bioflocculant was able to remove Congo Red, Direct Black and Methylene Blue at efficiencies of 98.5%, 97.9% and 72.3% respectively. CONCLUSIONS: The results of this study indicated that the marine strain Alteromonas sp. is a good candidate for the production of a novel bioflocculant and suggested its potential industrial utility for biotechnological processes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12896-017-0404-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5693566 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-56935662017-11-24 Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment Chen, Zhen Li, Zhipeng Liu, Peize Liu, Yu Wang, Yuanpeng Li, Qingbiao He, Ning BMC Biotechnol Research Article BACKGROUND: The identification of microorganisms with excellent flocculant-producing capability and optimization of the fermentation process are necessary for the wide-scale application of bioflocculants. Thus, we evaluated the flocculant-producing ability of a novel strain identified by the screening of marine bacteria, and we report for the first time the properties of the bioflocculant produced by Alteromonas sp. in the treatment of dye wastewater. RESULTS: A bioflocculant-producing bacterium was isolated from seawater and identified as Alteromonas sp. CGMCC 10612. The optimal carbon and nitrogen sources for the strain were 30 g/L glucose and 1.5 g/L wheat flour. In a 2-L fermenter, the flocculating activity and bioflocculant yield reached maximum values of 2575.4 U/mL and 11.18 g/L, respectively. The bioflocculant was separated and showed good heat and pH stability. The purified bioflocculant was a proteoglycan consisting of 69.61% carbohydrate and 21.56% protein (wt/wt). Infrared spectrometry further indicated the presence of hydroxyl, carboxyl and amino groups preferred for flocculation. The bioflocculant was a nanoparticle polymer with an average mass of 394,000 Da. The purified bioflocculant was able to remove Congo Red, Direct Black and Methylene Blue at efficiencies of 98.5%, 97.9% and 72.3% respectively. CONCLUSIONS: The results of this study indicated that the marine strain Alteromonas sp. is a good candidate for the production of a novel bioflocculant and suggested its potential industrial utility for biotechnological processes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12896-017-0404-z) contains supplementary material, which is available to authorized users. BioMed Central 2017-11-17 /pmc/articles/PMC5693566/ /pubmed/29149843 http://dx.doi.org/10.1186/s12896-017-0404-z Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Chen, Zhen Li, Zhipeng Liu, Peize Liu, Yu Wang, Yuanpeng Li, Qingbiao He, Ning Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment |
title | Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment |
title_full | Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment |
title_fullStr | Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment |
title_full_unstemmed | Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment |
title_short | Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment |
title_sort | characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693566/ https://www.ncbi.nlm.nih.gov/pubmed/29149843 http://dx.doi.org/10.1186/s12896-017-0404-z |
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