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Spatial regulation of contractility by Neuralized and Bearded during furrow invagination in Drosophila

Embryo-scale morphogenesis arises from patterned mechanical forces. During Drosophila gastrulation, actomyosin contractility drives apical constriction in ventral cells, leading to furrow formation and mesoderm invagination. It remains unclear whether and how mechanical properties of the ectoderm in...

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Autores principales: Perez-Mockus, Gantas, Mazouni, Khalil, Roca, Vanessa, Corradi, Giulia, Conte, Vito, Schweisguth, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693868/
https://www.ncbi.nlm.nih.gov/pubmed/29150614
http://dx.doi.org/10.1038/s41467-017-01482-8
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author Perez-Mockus, Gantas
Mazouni, Khalil
Roca, Vanessa
Corradi, Giulia
Conte, Vito
Schweisguth, François
author_facet Perez-Mockus, Gantas
Mazouni, Khalil
Roca, Vanessa
Corradi, Giulia
Conte, Vito
Schweisguth, François
author_sort Perez-Mockus, Gantas
collection PubMed
description Embryo-scale morphogenesis arises from patterned mechanical forces. During Drosophila gastrulation, actomyosin contractility drives apical constriction in ventral cells, leading to furrow formation and mesoderm invagination. It remains unclear whether and how mechanical properties of the ectoderm influence this process. Here, we show that Neuralized (Neur), an E3 ubiquitin ligase active in the mesoderm, regulates collective apical constriction and furrow formation. Conversely, the Bearded (Brd) proteins antagonize maternal Neur and lower medial–apical contractility in the ectoderm: in Brd-mutant embryos, the ventral furrow invaginates properly but rapidly unfolds as medial MyoII levels increase in the ectoderm. Increasing contractility in the ectoderm via activated Rho similarly triggers furrow unfolding whereas decreasing contractility restores furrow invagination in Brd-mutant embryos. Thus, the inhibition of Neur by Brd in the ectoderm differentiates the mechanics of the ectoderm from that of the mesoderm and patterns the activity of MyoII along the dorsal–ventral axis.
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spelling pubmed-56938682017-11-20 Spatial regulation of contractility by Neuralized and Bearded during furrow invagination in Drosophila Perez-Mockus, Gantas Mazouni, Khalil Roca, Vanessa Corradi, Giulia Conte, Vito Schweisguth, François Nat Commun Article Embryo-scale morphogenesis arises from patterned mechanical forces. During Drosophila gastrulation, actomyosin contractility drives apical constriction in ventral cells, leading to furrow formation and mesoderm invagination. It remains unclear whether and how mechanical properties of the ectoderm influence this process. Here, we show that Neuralized (Neur), an E3 ubiquitin ligase active in the mesoderm, regulates collective apical constriction and furrow formation. Conversely, the Bearded (Brd) proteins antagonize maternal Neur and lower medial–apical contractility in the ectoderm: in Brd-mutant embryos, the ventral furrow invaginates properly but rapidly unfolds as medial MyoII levels increase in the ectoderm. Increasing contractility in the ectoderm via activated Rho similarly triggers furrow unfolding whereas decreasing contractility restores furrow invagination in Brd-mutant embryos. Thus, the inhibition of Neur by Brd in the ectoderm differentiates the mechanics of the ectoderm from that of the mesoderm and patterns the activity of MyoII along the dorsal–ventral axis. Nature Publishing Group UK 2017-11-17 /pmc/articles/PMC5693868/ /pubmed/29150614 http://dx.doi.org/10.1038/s41467-017-01482-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Perez-Mockus, Gantas
Mazouni, Khalil
Roca, Vanessa
Corradi, Giulia
Conte, Vito
Schweisguth, François
Spatial regulation of contractility by Neuralized and Bearded during furrow invagination in Drosophila
title Spatial regulation of contractility by Neuralized and Bearded during furrow invagination in Drosophila
title_full Spatial regulation of contractility by Neuralized and Bearded during furrow invagination in Drosophila
title_fullStr Spatial regulation of contractility by Neuralized and Bearded during furrow invagination in Drosophila
title_full_unstemmed Spatial regulation of contractility by Neuralized and Bearded during furrow invagination in Drosophila
title_short Spatial regulation of contractility by Neuralized and Bearded during furrow invagination in Drosophila
title_sort spatial regulation of contractility by neuralized and bearded during furrow invagination in drosophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693868/
https://www.ncbi.nlm.nih.gov/pubmed/29150614
http://dx.doi.org/10.1038/s41467-017-01482-8
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