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Parathyroid Hormone-Related Protein Promotes Rat Stem Leydig Cell Differentiation

The regulatory factors for stem Leydig cell development are largely unknown. Herein, we reported that parathyroid hormone-related protein (PTHrP) may be a factor to regulate this process. The effects of PTHrP on rat stem Leydig cell proliferation and differentiation were investigated using a stem Le...

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Autores principales: Song, Tiantian, Wang, Yiyan, Li, Huitao, Chen, Lanlan, Liu, Jianpeng, Chen, Xianwu, Li, Xiaojun, Li, Xiaoheng, Li, Linxi, Lian, Qingquan, Ge, Ren-Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693895/
https://www.ncbi.nlm.nih.gov/pubmed/29180966
http://dx.doi.org/10.3389/fphys.2017.00911
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author Song, Tiantian
Wang, Yiyan
Li, Huitao
Chen, Lanlan
Liu, Jianpeng
Chen, Xianwu
Li, Xiaojun
Li, Xiaoheng
Li, Linxi
Lian, Qingquan
Ge, Ren-Shan
author_facet Song, Tiantian
Wang, Yiyan
Li, Huitao
Chen, Lanlan
Liu, Jianpeng
Chen, Xianwu
Li, Xiaojun
Li, Xiaoheng
Li, Linxi
Lian, Qingquan
Ge, Ren-Shan
author_sort Song, Tiantian
collection PubMed
description The regulatory factors for stem Leydig cell development are largely unknown. Herein, we reported that parathyroid hormone-related protein (PTHrP) may be a factor to regulate this process. The effects of PTHrP on rat stem Leydig cell proliferation and differentiation were investigated using a stem Leydig cell culture system and an ethane dimethane sulfonate (EDS)-treated in vivo Leydig cell regeneration model. PTHrP (1,000 pg/ml) significantly increased medium testosterone level and up-regulated STAR, CYP17A1, and 17β-HSD3 expressions. Co-treatment with PKA inhibitor H-89 or PKC inhibitor U73122 reversed PTHrP-mediated increase of testosterone production in vitro. Intratesticular injection of PTHrP (100 ng/testis) into the Leydig cell-depleted testis from post-EDS day 7 to 21 significantly increased serum testosterone level, up-regulated LHCGR, SCARB1, CYP11A1, 11β-HSD1, and CYP17A1 expressions. It also enlarged Leydig cell size without affecting PCNA-labeled Leydig cell number. This indicates that PTHrP promotes stem Leydig cell differentiation. PTHrP in vivo increased CREB and p-CREB levels, suggesting that PTHrP acts via a PKA-CREB signaling pathway. In conclusion, PTHrP stimulates stem Leydig cell differentiation without affecting its proliferation, showing its novel action and mechanism on rat stem Leydig cell development.
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spelling pubmed-56938952017-11-27 Parathyroid Hormone-Related Protein Promotes Rat Stem Leydig Cell Differentiation Song, Tiantian Wang, Yiyan Li, Huitao Chen, Lanlan Liu, Jianpeng Chen, Xianwu Li, Xiaojun Li, Xiaoheng Li, Linxi Lian, Qingquan Ge, Ren-Shan Front Physiol Physiology The regulatory factors for stem Leydig cell development are largely unknown. Herein, we reported that parathyroid hormone-related protein (PTHrP) may be a factor to regulate this process. The effects of PTHrP on rat stem Leydig cell proliferation and differentiation were investigated using a stem Leydig cell culture system and an ethane dimethane sulfonate (EDS)-treated in vivo Leydig cell regeneration model. PTHrP (1,000 pg/ml) significantly increased medium testosterone level and up-regulated STAR, CYP17A1, and 17β-HSD3 expressions. Co-treatment with PKA inhibitor H-89 or PKC inhibitor U73122 reversed PTHrP-mediated increase of testosterone production in vitro. Intratesticular injection of PTHrP (100 ng/testis) into the Leydig cell-depleted testis from post-EDS day 7 to 21 significantly increased serum testosterone level, up-regulated LHCGR, SCARB1, CYP11A1, 11β-HSD1, and CYP17A1 expressions. It also enlarged Leydig cell size without affecting PCNA-labeled Leydig cell number. This indicates that PTHrP promotes stem Leydig cell differentiation. PTHrP in vivo increased CREB and p-CREB levels, suggesting that PTHrP acts via a PKA-CREB signaling pathway. In conclusion, PTHrP stimulates stem Leydig cell differentiation without affecting its proliferation, showing its novel action and mechanism on rat stem Leydig cell development. Frontiers Media S.A. 2017-11-13 /pmc/articles/PMC5693895/ /pubmed/29180966 http://dx.doi.org/10.3389/fphys.2017.00911 Text en Copyright © 2017 Song, Wang, Li, Chen, Liu, Chen, Li, Li, Li, Lian and Ge. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Song, Tiantian
Wang, Yiyan
Li, Huitao
Chen, Lanlan
Liu, Jianpeng
Chen, Xianwu
Li, Xiaojun
Li, Xiaoheng
Li, Linxi
Lian, Qingquan
Ge, Ren-Shan
Parathyroid Hormone-Related Protein Promotes Rat Stem Leydig Cell Differentiation
title Parathyroid Hormone-Related Protein Promotes Rat Stem Leydig Cell Differentiation
title_full Parathyroid Hormone-Related Protein Promotes Rat Stem Leydig Cell Differentiation
title_fullStr Parathyroid Hormone-Related Protein Promotes Rat Stem Leydig Cell Differentiation
title_full_unstemmed Parathyroid Hormone-Related Protein Promotes Rat Stem Leydig Cell Differentiation
title_short Parathyroid Hormone-Related Protein Promotes Rat Stem Leydig Cell Differentiation
title_sort parathyroid hormone-related protein promotes rat stem leydig cell differentiation
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693895/
https://www.ncbi.nlm.nih.gov/pubmed/29180966
http://dx.doi.org/10.3389/fphys.2017.00911
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