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Escherichia coli Fails to Efficiently Maintain the Activity of an Important Flavin Monooxygenase in Recombinant Overexpression

This paper describes the measurement and analysis of in vivo activity and stability of cyclohexanone monooxygenase from Acinetobacter sp. NCIMB 9871 (CHMO), a model Baeyer–Villiger monooxygenase, in the recombinant host Escherichia coli. This enzyme was often described as poorly stable in vitro, and...

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Detalles Bibliográficos
Autores principales: Milker, Sofia, Goncalves, Leticia C. P., Fink, Michael J., Rudroff, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693912/
https://www.ncbi.nlm.nih.gov/pubmed/29180987
http://dx.doi.org/10.3389/fmicb.2017.02201

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