Cargando…

Inhibition of myeloid differentiation primary response protein 88 provides neuroprotection in early brain injury following experimental subarachnoid hemorrhage

Accumulating of evidence suggests that activation of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) exacerbates early brain injury (EBI) following subarachnoid hemorrhage (SAH) by provoking pro-inflammatory and pro-apoptotic signaling. Myeloid differentiation primary re...

Descripción completa

Detalles Bibliográficos
Autores principales: Yan, Huiying, Zhang, Dingding, Wei, Yongxiang, Ni, Hongbin, Liang, Weibang, Zhang, Huasheng, Hao, Shuangying, Jin, Wei, Li, Kuanyu, Hang, Chun-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693947/
https://www.ncbi.nlm.nih.gov/pubmed/29150630
http://dx.doi.org/10.1038/s41598-017-16124-8
_version_ 1783280022479437824
author Yan, Huiying
Zhang, Dingding
Wei, Yongxiang
Ni, Hongbin
Liang, Weibang
Zhang, Huasheng
Hao, Shuangying
Jin, Wei
Li, Kuanyu
Hang, Chun-Hua
author_facet Yan, Huiying
Zhang, Dingding
Wei, Yongxiang
Ni, Hongbin
Liang, Weibang
Zhang, Huasheng
Hao, Shuangying
Jin, Wei
Li, Kuanyu
Hang, Chun-Hua
author_sort Yan, Huiying
collection PubMed
description Accumulating of evidence suggests that activation of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) exacerbates early brain injury (EBI) following subarachnoid hemorrhage (SAH) by provoking pro-inflammatory and pro-apoptotic signaling. Myeloid differentiation primary response protein 88 (MyD88) is an endogenous adaptor protein in the toll-like receptors (TLRs) and interleukin (IL) -1β family signaling pathways and acts as a bottle neck in the NF-κB and MAPK pathways. Here, we used ST2825, a selective inhibitor of MyD88, to clarify whether inhibiting MyD88 could provide neuroprotection in EBI following SAH. Our results showed that the expression of MyD88 was markedly increased at 24 h post SAH. Intracerebroventricular injection of ST2825 significantly reduced the expression of MyD88 at 24 h post SAH. Involvement of MAPKs and NF-κB signaling pathways was revealed that ST2825 inhibited SAH-induced phosphorylation of TAK1, p38 and JNK, the nuclear translocation of NF-κB p65, and degradation of IκBα. Further, ST2825 administration diminished the SAH-induced inflammatory response and apoptosis. As a result, SAH-induced EBI was alleviated and neurological deficits caused by SAH were reversed. Our findings suggest that MyD88 inhibition confers marked neuroprotection against EBI following SAH. Therefore, MyD88 might be a promising new molecular target for the treatment of SAH.
format Online
Article
Text
id pubmed-5693947
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-56939472017-11-27 Inhibition of myeloid differentiation primary response protein 88 provides neuroprotection in early brain injury following experimental subarachnoid hemorrhage Yan, Huiying Zhang, Dingding Wei, Yongxiang Ni, Hongbin Liang, Weibang Zhang, Huasheng Hao, Shuangying Jin, Wei Li, Kuanyu Hang, Chun-Hua Sci Rep Article Accumulating of evidence suggests that activation of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) exacerbates early brain injury (EBI) following subarachnoid hemorrhage (SAH) by provoking pro-inflammatory and pro-apoptotic signaling. Myeloid differentiation primary response protein 88 (MyD88) is an endogenous adaptor protein in the toll-like receptors (TLRs) and interleukin (IL) -1β family signaling pathways and acts as a bottle neck in the NF-κB and MAPK pathways. Here, we used ST2825, a selective inhibitor of MyD88, to clarify whether inhibiting MyD88 could provide neuroprotection in EBI following SAH. Our results showed that the expression of MyD88 was markedly increased at 24 h post SAH. Intracerebroventricular injection of ST2825 significantly reduced the expression of MyD88 at 24 h post SAH. Involvement of MAPKs and NF-κB signaling pathways was revealed that ST2825 inhibited SAH-induced phosphorylation of TAK1, p38 and JNK, the nuclear translocation of NF-κB p65, and degradation of IκBα. Further, ST2825 administration diminished the SAH-induced inflammatory response and apoptosis. As a result, SAH-induced EBI was alleviated and neurological deficits caused by SAH were reversed. Our findings suggest that MyD88 inhibition confers marked neuroprotection against EBI following SAH. Therefore, MyD88 might be a promising new molecular target for the treatment of SAH. Nature Publishing Group UK 2017-11-17 /pmc/articles/PMC5693947/ /pubmed/29150630 http://dx.doi.org/10.1038/s41598-017-16124-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yan, Huiying
Zhang, Dingding
Wei, Yongxiang
Ni, Hongbin
Liang, Weibang
Zhang, Huasheng
Hao, Shuangying
Jin, Wei
Li, Kuanyu
Hang, Chun-Hua
Inhibition of myeloid differentiation primary response protein 88 provides neuroprotection in early brain injury following experimental subarachnoid hemorrhage
title Inhibition of myeloid differentiation primary response protein 88 provides neuroprotection in early brain injury following experimental subarachnoid hemorrhage
title_full Inhibition of myeloid differentiation primary response protein 88 provides neuroprotection in early brain injury following experimental subarachnoid hemorrhage
title_fullStr Inhibition of myeloid differentiation primary response protein 88 provides neuroprotection in early brain injury following experimental subarachnoid hemorrhage
title_full_unstemmed Inhibition of myeloid differentiation primary response protein 88 provides neuroprotection in early brain injury following experimental subarachnoid hemorrhage
title_short Inhibition of myeloid differentiation primary response protein 88 provides neuroprotection in early brain injury following experimental subarachnoid hemorrhage
title_sort inhibition of myeloid differentiation primary response protein 88 provides neuroprotection in early brain injury following experimental subarachnoid hemorrhage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693947/
https://www.ncbi.nlm.nih.gov/pubmed/29150630
http://dx.doi.org/10.1038/s41598-017-16124-8
work_keys_str_mv AT yanhuiying inhibitionofmyeloiddifferentiationprimaryresponseprotein88providesneuroprotectioninearlybraininjuryfollowingexperimentalsubarachnoidhemorrhage
AT zhangdingding inhibitionofmyeloiddifferentiationprimaryresponseprotein88providesneuroprotectioninearlybraininjuryfollowingexperimentalsubarachnoidhemorrhage
AT weiyongxiang inhibitionofmyeloiddifferentiationprimaryresponseprotein88providesneuroprotectioninearlybraininjuryfollowingexperimentalsubarachnoidhemorrhage
AT nihongbin inhibitionofmyeloiddifferentiationprimaryresponseprotein88providesneuroprotectioninearlybraininjuryfollowingexperimentalsubarachnoidhemorrhage
AT liangweibang inhibitionofmyeloiddifferentiationprimaryresponseprotein88providesneuroprotectioninearlybraininjuryfollowingexperimentalsubarachnoidhemorrhage
AT zhanghuasheng inhibitionofmyeloiddifferentiationprimaryresponseprotein88providesneuroprotectioninearlybraininjuryfollowingexperimentalsubarachnoidhemorrhage
AT haoshuangying inhibitionofmyeloiddifferentiationprimaryresponseprotein88providesneuroprotectioninearlybraininjuryfollowingexperimentalsubarachnoidhemorrhage
AT jinwei inhibitionofmyeloiddifferentiationprimaryresponseprotein88providesneuroprotectioninearlybraininjuryfollowingexperimentalsubarachnoidhemorrhage
AT likuanyu inhibitionofmyeloiddifferentiationprimaryresponseprotein88providesneuroprotectioninearlybraininjuryfollowingexperimentalsubarachnoidhemorrhage
AT hangchunhua inhibitionofmyeloiddifferentiationprimaryresponseprotein88providesneuroprotectioninearlybraininjuryfollowingexperimentalsubarachnoidhemorrhage