Cargando…
Transcriptome Analysis on Single Small Yellow Follicles Reveals That Wnt4 Is Involved in Chicken Follicle Selection
Ovarian follicle selection is an important process impacting the laying performance and fecundity of hens, and is regulated by follicle-stimulating hormone (FSH) through binding to its receptor [follicle-stimulating hormone receptor (FSHR)]. In laying hens, the small yellow follicle (6–8 mm in diame...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5694752/ https://www.ncbi.nlm.nih.gov/pubmed/29187833 http://dx.doi.org/10.3389/fendo.2017.00317 |
_version_ | 1783280190858723328 |
---|---|
author | Wang, Yiya Chen, Qiuyue Liu, Zemin Guo, Xiaoli Du, Yanzhi Yuan, Zhenjie Guo, Miao Kang, Li Sun, Yi Jiang, Yunliang |
author_facet | Wang, Yiya Chen, Qiuyue Liu, Zemin Guo, Xiaoli Du, Yanzhi Yuan, Zhenjie Guo, Miao Kang, Li Sun, Yi Jiang, Yunliang |
author_sort | Wang, Yiya |
collection | PubMed |
description | Ovarian follicle selection is an important process impacting the laying performance and fecundity of hens, and is regulated by follicle-stimulating hormone (FSH) through binding to its receptor [follicle-stimulating hormone receptor (FSHR)]. In laying hens, the small yellow follicle (6–8 mm in diameter) with the highest expression of FSHR will be recruited into the preovulatory hierarchy during ovarian follicle development. The study of molecular mechanism of chicken follicle selection is helpful for the identification of genes underlying egg-laying traits in chicken and other poultry species. Herein, the transcriptomes of chicken small yellow follicles differing in the mRNA expression of FSHR were compared, and a total of 17,993 genes were identified in 3 pairs of small yellow follicles. The Wnt signaling pathway was significantly enriched in the follicles with the greatest fold change in FSHR expression. In this pathway, the expression level of Wnt4 mRNA was significantly upregulated with a log(2)(fold change) of 2.12. We further investigated the expression, function, and regulation of Wnt4 during chicken follicle selection and found that Wnt4 mRNA reached its peak in small yellow follicles; Wnt4 stimulated the proliferation of follicular granulosa cells (GCs), increased the expression of StAR and CYP11A1 mRNA in prehierarchical and hierarchical follicles, increased the expression of FSHR mRNA, and decreased the expression of anti-Müllerian hormone and OCLN mRNA. Treatment with FSH significantly increased Wnt4 expression in GCs. Moreover, Wnt4 facilitated the effects of FSH on the production of progesterone (P4) and the mRNA expression of steroidogenic enzyme genes in the GCs of hierarchical follicles, but inhibited the effects of FSH in the GCs of prehierarchical follicles. Collectively, these data suggest that Wnt4 plays an important role in chicken follicle selection by stimulating GC proliferation and steroidogenesis. This study provides a theoretical basis for improving the egg-laying performance of chicken and a reference for the elucidation of the molecular mechanism of follicular selection in mammals. |
format | Online Article Text |
id | pubmed-5694752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56947522017-11-29 Transcriptome Analysis on Single Small Yellow Follicles Reveals That Wnt4 Is Involved in Chicken Follicle Selection Wang, Yiya Chen, Qiuyue Liu, Zemin Guo, Xiaoli Du, Yanzhi Yuan, Zhenjie Guo, Miao Kang, Li Sun, Yi Jiang, Yunliang Front Endocrinol (Lausanne) Endocrinology Ovarian follicle selection is an important process impacting the laying performance and fecundity of hens, and is regulated by follicle-stimulating hormone (FSH) through binding to its receptor [follicle-stimulating hormone receptor (FSHR)]. In laying hens, the small yellow follicle (6–8 mm in diameter) with the highest expression of FSHR will be recruited into the preovulatory hierarchy during ovarian follicle development. The study of molecular mechanism of chicken follicle selection is helpful for the identification of genes underlying egg-laying traits in chicken and other poultry species. Herein, the transcriptomes of chicken small yellow follicles differing in the mRNA expression of FSHR were compared, and a total of 17,993 genes were identified in 3 pairs of small yellow follicles. The Wnt signaling pathway was significantly enriched in the follicles with the greatest fold change in FSHR expression. In this pathway, the expression level of Wnt4 mRNA was significantly upregulated with a log(2)(fold change) of 2.12. We further investigated the expression, function, and regulation of Wnt4 during chicken follicle selection and found that Wnt4 mRNA reached its peak in small yellow follicles; Wnt4 stimulated the proliferation of follicular granulosa cells (GCs), increased the expression of StAR and CYP11A1 mRNA in prehierarchical and hierarchical follicles, increased the expression of FSHR mRNA, and decreased the expression of anti-Müllerian hormone and OCLN mRNA. Treatment with FSH significantly increased Wnt4 expression in GCs. Moreover, Wnt4 facilitated the effects of FSH on the production of progesterone (P4) and the mRNA expression of steroidogenic enzyme genes in the GCs of hierarchical follicles, but inhibited the effects of FSH in the GCs of prehierarchical follicles. Collectively, these data suggest that Wnt4 plays an important role in chicken follicle selection by stimulating GC proliferation and steroidogenesis. This study provides a theoretical basis for improving the egg-laying performance of chicken and a reference for the elucidation of the molecular mechanism of follicular selection in mammals. Frontiers Media S.A. 2017-11-15 /pmc/articles/PMC5694752/ /pubmed/29187833 http://dx.doi.org/10.3389/fendo.2017.00317 Text en Copyright © 2017 Wang, Chen, Liu, Guo, Du, Yuan, Guo, Kang, Sun and Jiang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Wang, Yiya Chen, Qiuyue Liu, Zemin Guo, Xiaoli Du, Yanzhi Yuan, Zhenjie Guo, Miao Kang, Li Sun, Yi Jiang, Yunliang Transcriptome Analysis on Single Small Yellow Follicles Reveals That Wnt4 Is Involved in Chicken Follicle Selection |
title | Transcriptome Analysis on Single Small Yellow Follicles Reveals That Wnt4 Is Involved in Chicken Follicle Selection |
title_full | Transcriptome Analysis on Single Small Yellow Follicles Reveals That Wnt4 Is Involved in Chicken Follicle Selection |
title_fullStr | Transcriptome Analysis on Single Small Yellow Follicles Reveals That Wnt4 Is Involved in Chicken Follicle Selection |
title_full_unstemmed | Transcriptome Analysis on Single Small Yellow Follicles Reveals That Wnt4 Is Involved in Chicken Follicle Selection |
title_short | Transcriptome Analysis on Single Small Yellow Follicles Reveals That Wnt4 Is Involved in Chicken Follicle Selection |
title_sort | transcriptome analysis on single small yellow follicles reveals that wnt4 is involved in chicken follicle selection |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5694752/ https://www.ncbi.nlm.nih.gov/pubmed/29187833 http://dx.doi.org/10.3389/fendo.2017.00317 |
work_keys_str_mv | AT wangyiya transcriptomeanalysisonsinglesmallyellowfolliclesrevealsthatwnt4isinvolvedinchickenfollicleselection AT chenqiuyue transcriptomeanalysisonsinglesmallyellowfolliclesrevealsthatwnt4isinvolvedinchickenfollicleselection AT liuzemin transcriptomeanalysisonsinglesmallyellowfolliclesrevealsthatwnt4isinvolvedinchickenfollicleselection AT guoxiaoli transcriptomeanalysisonsinglesmallyellowfolliclesrevealsthatwnt4isinvolvedinchickenfollicleselection AT duyanzhi transcriptomeanalysisonsinglesmallyellowfolliclesrevealsthatwnt4isinvolvedinchickenfollicleselection AT yuanzhenjie transcriptomeanalysisonsinglesmallyellowfolliclesrevealsthatwnt4isinvolvedinchickenfollicleselection AT guomiao transcriptomeanalysisonsinglesmallyellowfolliclesrevealsthatwnt4isinvolvedinchickenfollicleselection AT kangli transcriptomeanalysisonsinglesmallyellowfolliclesrevealsthatwnt4isinvolvedinchickenfollicleselection AT sunyi transcriptomeanalysisonsinglesmallyellowfolliclesrevealsthatwnt4isinvolvedinchickenfollicleselection AT jiangyunliang transcriptomeanalysisonsinglesmallyellowfolliclesrevealsthatwnt4isinvolvedinchickenfollicleselection |