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Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome

We report data associated with the identification of three polyhydroxyalkanoate synthase genes (phaC) isolated from the marine bacteria metagenome of Aaptos aaptos marine sponge in the waters of Bidong Island, Terengganu, Malaysia. Our data describe the extraction of bacterial metagenome from sponge...

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Autores principales: Amelia, Tan Suet May, Amirul, Al-Ashraf Abdullah, Bhubalan, Kesaven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5694960/
https://www.ncbi.nlm.nih.gov/pubmed/29188224
http://dx.doi.org/10.1016/j.dib.2017.11.011
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author Amelia, Tan Suet May
Amirul, Al-Ashraf Abdullah
Bhubalan, Kesaven
author_facet Amelia, Tan Suet May
Amirul, Al-Ashraf Abdullah
Bhubalan, Kesaven
author_sort Amelia, Tan Suet May
collection PubMed
description We report data associated with the identification of three polyhydroxyalkanoate synthase genes (phaC) isolated from the marine bacteria metagenome of Aaptos aaptos marine sponge in the waters of Bidong Island, Terengganu, Malaysia. Our data describe the extraction of bacterial metagenome from sponge tissue, measurement of purity and concentration of extracted metagenome, polymerase chain reaction (PCR)-mediated amplification using degenerate primers targeting Class I and II phaC genes, sequencing at First BASE Laboratories Sdn Bhd, and phylogenetic analysis of identified and known phaC genes. The partial nucleotide sequences were aligned, refined, compared with the Basic Local Alignment Search Tool (BLAST) databases, and released online in GenBank. The data include the identified partial putative phaC and their GenBank accession numbers, which are Rhodocista sp. phaC (MF457754), Pseudomonas sp. phaC (MF437016), and an uncultured bacterium AR5-9d_16 phaC (MF457753).
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spelling pubmed-56949602017-11-29 Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome Amelia, Tan Suet May Amirul, Al-Ashraf Abdullah Bhubalan, Kesaven Data Brief Genetics, Genomics and Molecular Biology We report data associated with the identification of three polyhydroxyalkanoate synthase genes (phaC) isolated from the marine bacteria metagenome of Aaptos aaptos marine sponge in the waters of Bidong Island, Terengganu, Malaysia. Our data describe the extraction of bacterial metagenome from sponge tissue, measurement of purity and concentration of extracted metagenome, polymerase chain reaction (PCR)-mediated amplification using degenerate primers targeting Class I and II phaC genes, sequencing at First BASE Laboratories Sdn Bhd, and phylogenetic analysis of identified and known phaC genes. The partial nucleotide sequences were aligned, refined, compared with the Basic Local Alignment Search Tool (BLAST) databases, and released online in GenBank. The data include the identified partial putative phaC and their GenBank accession numbers, which are Rhodocista sp. phaC (MF457754), Pseudomonas sp. phaC (MF437016), and an uncultured bacterium AR5-9d_16 phaC (MF457753). Elsevier 2017-11-08 /pmc/articles/PMC5694960/ /pubmed/29188224 http://dx.doi.org/10.1016/j.dib.2017.11.011 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Genetics, Genomics and Molecular Biology
Amelia, Tan Suet May
Amirul, Al-Ashraf Abdullah
Bhubalan, Kesaven
Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome
title Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome
title_full Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome
title_fullStr Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome
title_full_unstemmed Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome
title_short Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome
title_sort data on partial polyhydroxyalkanoate synthase genes (phac) mined from aaptos aaptos marine sponge-associated bacteria metagenome
topic Genetics, Genomics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5694960/
https://www.ncbi.nlm.nih.gov/pubmed/29188224
http://dx.doi.org/10.1016/j.dib.2017.11.011
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