Cargando…
Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome
We report data associated with the identification of three polyhydroxyalkanoate synthase genes (phaC) isolated from the marine bacteria metagenome of Aaptos aaptos marine sponge in the waters of Bidong Island, Terengganu, Malaysia. Our data describe the extraction of bacterial metagenome from sponge...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5694960/ https://www.ncbi.nlm.nih.gov/pubmed/29188224 http://dx.doi.org/10.1016/j.dib.2017.11.011 |
_version_ | 1783280226507161600 |
---|---|
author | Amelia, Tan Suet May Amirul, Al-Ashraf Abdullah Bhubalan, Kesaven |
author_facet | Amelia, Tan Suet May Amirul, Al-Ashraf Abdullah Bhubalan, Kesaven |
author_sort | Amelia, Tan Suet May |
collection | PubMed |
description | We report data associated with the identification of three polyhydroxyalkanoate synthase genes (phaC) isolated from the marine bacteria metagenome of Aaptos aaptos marine sponge in the waters of Bidong Island, Terengganu, Malaysia. Our data describe the extraction of bacterial metagenome from sponge tissue, measurement of purity and concentration of extracted metagenome, polymerase chain reaction (PCR)-mediated amplification using degenerate primers targeting Class I and II phaC genes, sequencing at First BASE Laboratories Sdn Bhd, and phylogenetic analysis of identified and known phaC genes. The partial nucleotide sequences were aligned, refined, compared with the Basic Local Alignment Search Tool (BLAST) databases, and released online in GenBank. The data include the identified partial putative phaC and their GenBank accession numbers, which are Rhodocista sp. phaC (MF457754), Pseudomonas sp. phaC (MF437016), and an uncultured bacterium AR5-9d_16 phaC (MF457753). |
format | Online Article Text |
id | pubmed-5694960 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-56949602017-11-29 Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome Amelia, Tan Suet May Amirul, Al-Ashraf Abdullah Bhubalan, Kesaven Data Brief Genetics, Genomics and Molecular Biology We report data associated with the identification of three polyhydroxyalkanoate synthase genes (phaC) isolated from the marine bacteria metagenome of Aaptos aaptos marine sponge in the waters of Bidong Island, Terengganu, Malaysia. Our data describe the extraction of bacterial metagenome from sponge tissue, measurement of purity and concentration of extracted metagenome, polymerase chain reaction (PCR)-mediated amplification using degenerate primers targeting Class I and II phaC genes, sequencing at First BASE Laboratories Sdn Bhd, and phylogenetic analysis of identified and known phaC genes. The partial nucleotide sequences were aligned, refined, compared with the Basic Local Alignment Search Tool (BLAST) databases, and released online in GenBank. The data include the identified partial putative phaC and their GenBank accession numbers, which are Rhodocista sp. phaC (MF457754), Pseudomonas sp. phaC (MF437016), and an uncultured bacterium AR5-9d_16 phaC (MF457753). Elsevier 2017-11-08 /pmc/articles/PMC5694960/ /pubmed/29188224 http://dx.doi.org/10.1016/j.dib.2017.11.011 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Genetics, Genomics and Molecular Biology Amelia, Tan Suet May Amirul, Al-Ashraf Abdullah Bhubalan, Kesaven Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome |
title | Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome |
title_full | Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome |
title_fullStr | Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome |
title_full_unstemmed | Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome |
title_short | Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome |
title_sort | data on partial polyhydroxyalkanoate synthase genes (phac) mined from aaptos aaptos marine sponge-associated bacteria metagenome |
topic | Genetics, Genomics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5694960/ https://www.ncbi.nlm.nih.gov/pubmed/29188224 http://dx.doi.org/10.1016/j.dib.2017.11.011 |
work_keys_str_mv | AT ameliatansuetmay dataonpartialpolyhydroxyalkanoatesynthasegenesphacminedfromaaptosaaptosmarinespongeassociatedbacteriametagenome AT amirulalashrafabdullah dataonpartialpolyhydroxyalkanoatesynthasegenesphacminedfromaaptosaaptosmarinespongeassociatedbacteriametagenome AT bhubalankesaven dataonpartialpolyhydroxyalkanoatesynthasegenesphacminedfromaaptosaaptosmarinespongeassociatedbacteriametagenome |