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Apelin Gene Therapy Increases Autophagy via Activation of Sirtuin 3 in Diabetic Heart
Heart failure is the leading cause of death in diabetic patients. Recently we showed that apelin gene therapy attenuates heart failure following myocardial infarction. This study further explored the potential mechanisms by which apelin may reduce cardiac injury in Postmyocardial infarction (MI)) mo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695036/ https://www.ncbi.nlm.nih.gov/pubmed/29167823 http://dx.doi.org/10.17140/DROJ-1-115 |
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author | Hou, Xuwei Zeng, Heng Tuo, Qin-Hui Liao, Daun-Fang Chen, Jian-Xiong |
author_facet | Hou, Xuwei Zeng, Heng Tuo, Qin-Hui Liao, Daun-Fang Chen, Jian-Xiong |
author_sort | Hou, Xuwei |
collection | PubMed |
description | Heart failure is the leading cause of death in diabetic patients. Recently we showed that apelin gene therapy attenuates heart failure following myocardial infarction. This study further explored the potential mechanisms by which apelin may reduce cardiac injury in Postmyocardial infarction (MI)) model of diabetes. Wild type and Sirt3 knockout (Sirt3 KO) mice were induced into diabetes by intra-peritoneal (i.p.) Streptozotocin (STZ). STZ mice were then subjected to MI followed by immediate intramyocardial injection with Adenovirus-apelin (Ad-apelin). Ad-apelin treatment resulted in over expression of apelin in the ischemic hearts of STZ mice. Apelin over expression led to a significant increase in Sirt3 expression. Apelin over expression significantly reduced gp91(phox) expression. This was accompanied by a significant reduction of reactive oxygen species formation. Ad-apelin treatment also dramatically reduced NF-κb-p65 expression in WT-STZ mice. Over expression of apelin further enhanced autophagy markers (LC3-II and beclin-1) expression in post-MI heart. Most intriguingly, knockout of Sirt3 in STZ mice abolished these beneficial effects of apelin treatment. In vitro, knockout of Sirt3 in EPCs significantly enhanced high glucose-induced ROS formation. Conversely, treatment of Sirt3 KO-EPCs with NADPH oxidase inhibitor led to two fold increase in LC3-II levels. Our studies demonstrate that apelin increases autophagy via up regulation of Sirt3 and suppression of ROS-NF-κb pathway in diabetic heart. |
format | Online Article Text |
id | pubmed-5695036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-56950362017-11-20 Apelin Gene Therapy Increases Autophagy via Activation of Sirtuin 3 in Diabetic Heart Hou, Xuwei Zeng, Heng Tuo, Qin-Hui Liao, Daun-Fang Chen, Jian-Xiong Diabetes Res (Fairfax) Article Heart failure is the leading cause of death in diabetic patients. Recently we showed that apelin gene therapy attenuates heart failure following myocardial infarction. This study further explored the potential mechanisms by which apelin may reduce cardiac injury in Postmyocardial infarction (MI)) model of diabetes. Wild type and Sirt3 knockout (Sirt3 KO) mice were induced into diabetes by intra-peritoneal (i.p.) Streptozotocin (STZ). STZ mice were then subjected to MI followed by immediate intramyocardial injection with Adenovirus-apelin (Ad-apelin). Ad-apelin treatment resulted in over expression of apelin in the ischemic hearts of STZ mice. Apelin over expression led to a significant increase in Sirt3 expression. Apelin over expression significantly reduced gp91(phox) expression. This was accompanied by a significant reduction of reactive oxygen species formation. Ad-apelin treatment also dramatically reduced NF-κb-p65 expression in WT-STZ mice. Over expression of apelin further enhanced autophagy markers (LC3-II and beclin-1) expression in post-MI heart. Most intriguingly, knockout of Sirt3 in STZ mice abolished these beneficial effects of apelin treatment. In vitro, knockout of Sirt3 in EPCs significantly enhanced high glucose-induced ROS formation. Conversely, treatment of Sirt3 KO-EPCs with NADPH oxidase inhibitor led to two fold increase in LC3-II levels. Our studies demonstrate that apelin increases autophagy via up regulation of Sirt3 and suppression of ROS-NF-κb pathway in diabetic heart. 2015-08-21 2015-10 /pmc/articles/PMC5695036/ /pubmed/29167823 http://dx.doi.org/10.17140/DROJ-1-115 Text en http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Hou, Xuwei Zeng, Heng Tuo, Qin-Hui Liao, Daun-Fang Chen, Jian-Xiong Apelin Gene Therapy Increases Autophagy via Activation of Sirtuin 3 in Diabetic Heart |
title | Apelin Gene Therapy Increases Autophagy via Activation of Sirtuin 3 in Diabetic Heart |
title_full | Apelin Gene Therapy Increases Autophagy via Activation of Sirtuin 3 in Diabetic Heart |
title_fullStr | Apelin Gene Therapy Increases Autophagy via Activation of Sirtuin 3 in Diabetic Heart |
title_full_unstemmed | Apelin Gene Therapy Increases Autophagy via Activation of Sirtuin 3 in Diabetic Heart |
title_short | Apelin Gene Therapy Increases Autophagy via Activation of Sirtuin 3 in Diabetic Heart |
title_sort | apelin gene therapy increases autophagy via activation of sirtuin 3 in diabetic heart |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695036/ https://www.ncbi.nlm.nih.gov/pubmed/29167823 http://dx.doi.org/10.17140/DROJ-1-115 |
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