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Asy2/Mer2: an evolutionarily conserved mediator of meiotic recombination, pairing, and global chromosome compaction

Meiosis is the cellular program by which a diploid cell gives rise to haploid gametes for sexual reproduction. Meiotic progression depends on tight physical and functional coupling of recombination steps at the DNA level with specific organizational features of meiotic-prophase chromosomes. The pres...

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Autores principales: Tessé, Sophie, Bourbon, Henri-Marc, Debuchy, Robert, Budin, Karine, Dubois, Emeline, Liangran, Zhang, Antoine, Romain, Piolot, Tristan, Kleckner, Nancy, Zickler, Denise, Espagne, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695089/
https://www.ncbi.nlm.nih.gov/pubmed/29021238
http://dx.doi.org/10.1101/gad.304543.117
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author Tessé, Sophie
Bourbon, Henri-Marc
Debuchy, Robert
Budin, Karine
Dubois, Emeline
Liangran, Zhang
Antoine, Romain
Piolot, Tristan
Kleckner, Nancy
Zickler, Denise
Espagne, Eric
author_facet Tessé, Sophie
Bourbon, Henri-Marc
Debuchy, Robert
Budin, Karine
Dubois, Emeline
Liangran, Zhang
Antoine, Romain
Piolot, Tristan
Kleckner, Nancy
Zickler, Denise
Espagne, Eric
author_sort Tessé, Sophie
collection PubMed
description Meiosis is the cellular program by which a diploid cell gives rise to haploid gametes for sexual reproduction. Meiotic progression depends on tight physical and functional coupling of recombination steps at the DNA level with specific organizational features of meiotic-prophase chromosomes. The present study reveals that every step of this coupling is mediated by a single molecule: Asy2/Mer2. We show that Mer2, identified so far only in budding and fission yeasts, is in fact evolutionarily conserved from fungi (Mer2/Rec15/Asy2/Bad42) to plants (PRD3/PAIR1) and mammals (IHO1). In yeasts, Mer2 mediates assembly of recombination–initiation complexes and double-strand breaks (DSBs). This role is conserved in the fungus Sordaria. However, functional analysis of 13 mer2 mutants and successive localization of Mer2 to axis, synaptonemal complex (SC), and chromatin revealed, in addition, three further important functions. First, after DSB formation, Mer2 is required for pairing by mediating homolog spatial juxtaposition, with implications for crossover (CO) patterning/interference. Second, Mer2 participates in the transfer/maintenance and release of recombination complexes to/from the SC central region. Third, after completion of recombination, potentially dependent on SUMOylation, Mer2 mediates global chromosome compaction and post-recombination chiasma development. Thus, beyond its role as a recombinosome–axis/SC linker molecule, Mer2 has important functions in relation to basic chromosome structure.
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spelling pubmed-56950892018-03-15 Asy2/Mer2: an evolutionarily conserved mediator of meiotic recombination, pairing, and global chromosome compaction Tessé, Sophie Bourbon, Henri-Marc Debuchy, Robert Budin, Karine Dubois, Emeline Liangran, Zhang Antoine, Romain Piolot, Tristan Kleckner, Nancy Zickler, Denise Espagne, Eric Genes Dev Research Paper Meiosis is the cellular program by which a diploid cell gives rise to haploid gametes for sexual reproduction. Meiotic progression depends on tight physical and functional coupling of recombination steps at the DNA level with specific organizational features of meiotic-prophase chromosomes. The present study reveals that every step of this coupling is mediated by a single molecule: Asy2/Mer2. We show that Mer2, identified so far only in budding and fission yeasts, is in fact evolutionarily conserved from fungi (Mer2/Rec15/Asy2/Bad42) to plants (PRD3/PAIR1) and mammals (IHO1). In yeasts, Mer2 mediates assembly of recombination–initiation complexes and double-strand breaks (DSBs). This role is conserved in the fungus Sordaria. However, functional analysis of 13 mer2 mutants and successive localization of Mer2 to axis, synaptonemal complex (SC), and chromatin revealed, in addition, three further important functions. First, after DSB formation, Mer2 is required for pairing by mediating homolog spatial juxtaposition, with implications for crossover (CO) patterning/interference. Second, Mer2 participates in the transfer/maintenance and release of recombination complexes to/from the SC central region. Third, after completion of recombination, potentially dependent on SUMOylation, Mer2 mediates global chromosome compaction and post-recombination chiasma development. Thus, beyond its role as a recombinosome–axis/SC linker molecule, Mer2 has important functions in relation to basic chromosome structure. Cold Spring Harbor Laboratory Press 2017-09-15 /pmc/articles/PMC5695089/ /pubmed/29021238 http://dx.doi.org/10.1101/gad.304543.117 Text en © 2017 Tessé et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Paper
Tessé, Sophie
Bourbon, Henri-Marc
Debuchy, Robert
Budin, Karine
Dubois, Emeline
Liangran, Zhang
Antoine, Romain
Piolot, Tristan
Kleckner, Nancy
Zickler, Denise
Espagne, Eric
Asy2/Mer2: an evolutionarily conserved mediator of meiotic recombination, pairing, and global chromosome compaction
title Asy2/Mer2: an evolutionarily conserved mediator of meiotic recombination, pairing, and global chromosome compaction
title_full Asy2/Mer2: an evolutionarily conserved mediator of meiotic recombination, pairing, and global chromosome compaction
title_fullStr Asy2/Mer2: an evolutionarily conserved mediator of meiotic recombination, pairing, and global chromosome compaction
title_full_unstemmed Asy2/Mer2: an evolutionarily conserved mediator of meiotic recombination, pairing, and global chromosome compaction
title_short Asy2/Mer2: an evolutionarily conserved mediator of meiotic recombination, pairing, and global chromosome compaction
title_sort asy2/mer2: an evolutionarily conserved mediator of meiotic recombination, pairing, and global chromosome compaction
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695089/
https://www.ncbi.nlm.nih.gov/pubmed/29021238
http://dx.doi.org/10.1101/gad.304543.117
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