Cargando…

A Laser-Activated Biocompatible Theranostic Nanoagent for Targeted Multimodal Imaging and Photothermal Therapy

Multifunctional nanoparticles have been reported for cancer detection and treatment currently. However, the accurate diagnosis and efficient treatment for tumors are still not satisfied. Here we report on the development of targeted phase change multimodal polymeric nanoparticles for the imaging and...

Descripción completa

Detalles Bibliográficos
Autores principales: Deng, Liming, Cai, Xiaojun, Sheng, Danli, Yang, Yang, Strohm, Eric M., Wang, Zhigang, Ran, Haitao, Wang, Dong, Zheng, Yuanyi, Li, Pan, Shang, Tingting, Ling, Yi, Wang, Fengjuan, Sun, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695140/
https://www.ncbi.nlm.nih.gov/pubmed/29158836
http://dx.doi.org/10.7150/thno.21283
_version_ 1783280257915158528
author Deng, Liming
Cai, Xiaojun
Sheng, Danli
Yang, Yang
Strohm, Eric M.
Wang, Zhigang
Ran, Haitao
Wang, Dong
Zheng, Yuanyi
Li, Pan
Shang, Tingting
Ling, Yi
Wang, Fengjuan
Sun, Yang
author_facet Deng, Liming
Cai, Xiaojun
Sheng, Danli
Yang, Yang
Strohm, Eric M.
Wang, Zhigang
Ran, Haitao
Wang, Dong
Zheng, Yuanyi
Li, Pan
Shang, Tingting
Ling, Yi
Wang, Fengjuan
Sun, Yang
author_sort Deng, Liming
collection PubMed
description Multifunctional nanoparticles have been reported for cancer detection and treatment currently. However, the accurate diagnosis and efficient treatment for tumors are still not satisfied. Here we report on the development of targeted phase change multimodal polymeric nanoparticles for the imaging and treatment of HER2-positive breast cancer. Methods: We evaluated the multimodal imaging capabilities of the prepared nanoparticles in vitro using agar-based phantoms. The targeting performance and cytotoxicity of the nanoparticles were examined in cell culture using SKBR3 (over-expressing HER2) and MDA-MB-231 (HER2 negative) cells. We then tested the magnetic resonance (MR)/ photoacoustic (PA)/ ultrasound (US)/ near-infrared fluorescence (NIRF) multimodal imaging properties and photothermal effect of the nanoparticles in vivo using a SKBR3 breast xenograft model in nude mice. Tissue histopathology and immunofluorescence were also conducted. Results: Both in vitro and in vivo systematical studies validated that the hybrid nanoparticles can be used as a superb MR/US/PA/NIRF contrast agent to simultaneously diagnose and guide tumor photothermal therapy (PTT). When irradiated by a near infrared laser, the liquid PFP vaporizes to a gas, rapidly expelling the contents and damaging surrounding tissues. The resulting micro-sized bubbles provide treatment validation through ultrasound imaging. Localization of DIR and SPIO in the tumor region facilitate photothermal therapy for targeted tumor destruction. The mice treated with HER2 targeted nanoparticles had a nearly complete response to treatment, while the controls showed continued tumor growth. Conclusion: This novel theranostic agent may provide better diagnostic imaging and therapeutic potential than current methods for treating HER2-positive breast cancer.
format Online
Article
Text
id pubmed-5695140
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Ivyspring International Publisher
record_format MEDLINE/PubMed
spelling pubmed-56951402017-11-20 A Laser-Activated Biocompatible Theranostic Nanoagent for Targeted Multimodal Imaging and Photothermal Therapy Deng, Liming Cai, Xiaojun Sheng, Danli Yang, Yang Strohm, Eric M. Wang, Zhigang Ran, Haitao Wang, Dong Zheng, Yuanyi Li, Pan Shang, Tingting Ling, Yi Wang, Fengjuan Sun, Yang Theranostics Research Paper Multifunctional nanoparticles have been reported for cancer detection and treatment currently. However, the accurate diagnosis and efficient treatment for tumors are still not satisfied. Here we report on the development of targeted phase change multimodal polymeric nanoparticles for the imaging and treatment of HER2-positive breast cancer. Methods: We evaluated the multimodal imaging capabilities of the prepared nanoparticles in vitro using agar-based phantoms. The targeting performance and cytotoxicity of the nanoparticles were examined in cell culture using SKBR3 (over-expressing HER2) and MDA-MB-231 (HER2 negative) cells. We then tested the magnetic resonance (MR)/ photoacoustic (PA)/ ultrasound (US)/ near-infrared fluorescence (NIRF) multimodal imaging properties and photothermal effect of the nanoparticles in vivo using a SKBR3 breast xenograft model in nude mice. Tissue histopathology and immunofluorescence were also conducted. Results: Both in vitro and in vivo systematical studies validated that the hybrid nanoparticles can be used as a superb MR/US/PA/NIRF contrast agent to simultaneously diagnose and guide tumor photothermal therapy (PTT). When irradiated by a near infrared laser, the liquid PFP vaporizes to a gas, rapidly expelling the contents and damaging surrounding tissues. The resulting micro-sized bubbles provide treatment validation through ultrasound imaging. Localization of DIR and SPIO in the tumor region facilitate photothermal therapy for targeted tumor destruction. The mice treated with HER2 targeted nanoparticles had a nearly complete response to treatment, while the controls showed continued tumor growth. Conclusion: This novel theranostic agent may provide better diagnostic imaging and therapeutic potential than current methods for treating HER2-positive breast cancer. Ivyspring International Publisher 2017-10-06 /pmc/articles/PMC5695140/ /pubmed/29158836 http://dx.doi.org/10.7150/thno.21283 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Deng, Liming
Cai, Xiaojun
Sheng, Danli
Yang, Yang
Strohm, Eric M.
Wang, Zhigang
Ran, Haitao
Wang, Dong
Zheng, Yuanyi
Li, Pan
Shang, Tingting
Ling, Yi
Wang, Fengjuan
Sun, Yang
A Laser-Activated Biocompatible Theranostic Nanoagent for Targeted Multimodal Imaging and Photothermal Therapy
title A Laser-Activated Biocompatible Theranostic Nanoagent for Targeted Multimodal Imaging and Photothermal Therapy
title_full A Laser-Activated Biocompatible Theranostic Nanoagent for Targeted Multimodal Imaging and Photothermal Therapy
title_fullStr A Laser-Activated Biocompatible Theranostic Nanoagent for Targeted Multimodal Imaging and Photothermal Therapy
title_full_unstemmed A Laser-Activated Biocompatible Theranostic Nanoagent for Targeted Multimodal Imaging and Photothermal Therapy
title_short A Laser-Activated Biocompatible Theranostic Nanoagent for Targeted Multimodal Imaging and Photothermal Therapy
title_sort laser-activated biocompatible theranostic nanoagent for targeted multimodal imaging and photothermal therapy
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695140/
https://www.ncbi.nlm.nih.gov/pubmed/29158836
http://dx.doi.org/10.7150/thno.21283
work_keys_str_mv AT dengliming alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT caixiaojun alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT shengdanli alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT yangyang alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT strohmericm alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT wangzhigang alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT ranhaitao alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT wangdong alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT zhengyuanyi alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT lipan alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT shangtingting alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT lingyi alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT wangfengjuan alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT sunyang alaseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT dengliming laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT caixiaojun laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT shengdanli laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT yangyang laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT strohmericm laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT wangzhigang laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT ranhaitao laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT wangdong laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT zhengyuanyi laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT lipan laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT shangtingting laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT lingyi laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT wangfengjuan laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy
AT sunyang laseractivatedbiocompatibletheranosticnanoagentfortargetedmultimodalimagingandphotothermaltherapy