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Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration

Our previous study revealed that mesoporous Ca-Si-based materials exhibited excellent osteoconduction because dissolved ions could form a layer of hydroxycarbonate apatite on the surface of the materials. However, the biological mechanisms underlying bone regeneration were largely unknown. The main...

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Autores principales: Zeng, Deliang, Zhang, Xingdi, Wang, Xiao, Cao, Lingyan, Zheng, Ao, Du, Jiahui, Li, Yongsheng, Huang, Qingfeng, Jiang, Xinquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695511/
https://www.ncbi.nlm.nih.gov/pubmed/29180865
http://dx.doi.org/10.2147/IJN.S144528
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author Zeng, Deliang
Zhang, Xingdi
Wang, Xiao
Cao, Lingyan
Zheng, Ao
Du, Jiahui
Li, Yongsheng
Huang, Qingfeng
Jiang, Xinquan
author_facet Zeng, Deliang
Zhang, Xingdi
Wang, Xiao
Cao, Lingyan
Zheng, Ao
Du, Jiahui
Li, Yongsheng
Huang, Qingfeng
Jiang, Xinquan
author_sort Zeng, Deliang
collection PubMed
description Our previous study revealed that mesoporous Ca-Si-based materials exhibited excellent osteoconduction because dissolved ions could form a layer of hydroxycarbonate apatite on the surface of the materials. However, the biological mechanisms underlying bone regeneration were largely unknown. The main aim of this study was to evaluate the osteogenic ability of large-pore mesoporous Ca-Si-based bioceramics (LPMSCs) by alkaline phosphatase assay, real-time PCR analysis, von Kossa, and alizarin red assay. Compared with large-pore mesoporous silica (LPMS), LPMSCs had a better effect on the osteogenic differentiation of dental pulp cells. LPMSC-2 and LPMSC-3 with higher calcium possessed better osteogenic abilities than LPMSC-1, which may be related to the calcium-sensing receptor pathway. Furthermore, the loading capacity for recombinant human platelet-derived growth factor-BB was satisfactory in LPMSCs. In vivo, the areas of new bone formation in the calvarial defect repair were increased in the LPMSC-2 and LPMSC-3 groups compared with the LPMSC-1 and LPMS groups. We concluded that LPMSC-2 and LPMSC-3 possessed both excellent osteogenic abilities and satisfactory loading capacities, which may be attributed to their moderate Ca/Si molar ratio. Therefore, LPMSCs with moderate Ca/Si molar ratio might be potential alterative grafts for craniomaxillofacial bone regeneration.
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spelling pubmed-56955112017-11-27 Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration Zeng, Deliang Zhang, Xingdi Wang, Xiao Cao, Lingyan Zheng, Ao Du, Jiahui Li, Yongsheng Huang, Qingfeng Jiang, Xinquan Int J Nanomedicine Original Research Our previous study revealed that mesoporous Ca-Si-based materials exhibited excellent osteoconduction because dissolved ions could form a layer of hydroxycarbonate apatite on the surface of the materials. However, the biological mechanisms underlying bone regeneration were largely unknown. The main aim of this study was to evaluate the osteogenic ability of large-pore mesoporous Ca-Si-based bioceramics (LPMSCs) by alkaline phosphatase assay, real-time PCR analysis, von Kossa, and alizarin red assay. Compared with large-pore mesoporous silica (LPMS), LPMSCs had a better effect on the osteogenic differentiation of dental pulp cells. LPMSC-2 and LPMSC-3 with higher calcium possessed better osteogenic abilities than LPMSC-1, which may be related to the calcium-sensing receptor pathway. Furthermore, the loading capacity for recombinant human platelet-derived growth factor-BB was satisfactory in LPMSCs. In vivo, the areas of new bone formation in the calvarial defect repair were increased in the LPMSC-2 and LPMSC-3 groups compared with the LPMSC-1 and LPMS groups. We concluded that LPMSC-2 and LPMSC-3 possessed both excellent osteogenic abilities and satisfactory loading capacities, which may be attributed to their moderate Ca/Si molar ratio. Therefore, LPMSCs with moderate Ca/Si molar ratio might be potential alterative grafts for craniomaxillofacial bone regeneration. Dove Medical Press 2017-11-15 /pmc/articles/PMC5695511/ /pubmed/29180865 http://dx.doi.org/10.2147/IJN.S144528 Text en © 2017 Zeng et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Zeng, Deliang
Zhang, Xingdi
Wang, Xiao
Cao, Lingyan
Zheng, Ao
Du, Jiahui
Li, Yongsheng
Huang, Qingfeng
Jiang, Xinquan
Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
title Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
title_full Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
title_fullStr Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
title_full_unstemmed Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
title_short Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
title_sort fabrication of large-pore mesoporous ca-si-based bioceramics for bone regeneration
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695511/
https://www.ncbi.nlm.nih.gov/pubmed/29180865
http://dx.doi.org/10.2147/IJN.S144528
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