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Virome analysis of two sympatric bat species (Desmodus rotundus and Molossus molossus) in French Guiana
Environmental disturbances in the Neotropics (e.g., deforestation, agriculture intensification, urbanization) contribute to an increasing risk of cross-species transmission of microorganisms and to disease outbreaks due to changing ecosystems of reservoir hosts. Although Amazonia encompasses the gre...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695591/ https://www.ncbi.nlm.nih.gov/pubmed/29117243 http://dx.doi.org/10.1371/journal.pone.0186943 |
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author | Salmier, Arielle Tirera, Sourakhata de Thoisy, Benoit Franc, Alain Darcissac, Edith Donato, Damien Bouchier, Christiane Lacoste, Vincent Lavergne, Anne |
author_facet | Salmier, Arielle Tirera, Sourakhata de Thoisy, Benoit Franc, Alain Darcissac, Edith Donato, Damien Bouchier, Christiane Lacoste, Vincent Lavergne, Anne |
author_sort | Salmier, Arielle |
collection | PubMed |
description | Environmental disturbances in the Neotropics (e.g., deforestation, agriculture intensification, urbanization) contribute to an increasing risk of cross-species transmission of microorganisms and to disease outbreaks due to changing ecosystems of reservoir hosts. Although Amazonia encompasses the greatest diversity of reservoir species, the outsized viral population diversity (virome) has yet to be investigated. Here, through a metagenomic approach, we identified 10,991 viral sequences in the saliva and feces of two bat species, Desmodus rotundus (hematophagous), trapped in two different caves surrounded by primary lowland forest, and Molossus molossus (insectivorous), trapped in forest and urban habitats. These sequences are related to 51 viral families known to infect a wide range of hosts (i.e., bacteria, plants, insects and vertebrates). Most viruses detected reflected the diet of bat species, with a high proportion of plant and insect-related viral families for M. molossus and a high proportion of vertebrate-related viral families for D. rotundus, highlighting its influence in shaping the viral diversity of bats. Lastly, we reconstructed the phylogenetic relationships for five vertebrate-related viral families (Nairoviridae, Circoviridae, Retroviridae, Herpesviridae, Papillomaviridae). The results showed highly supported clustering with other viral sequences of the same viral family hosted by other bat species, highlighting the potential association of viral diversity with the host’s diet. These findings provide significant insight into viral bat diversity in French Guiana belonging to the Amazonian biome and emphasize that habitats and the host’s dietary ecology may drive the viral diversity in the bat communities investigated. |
format | Online Article Text |
id | pubmed-5695591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56955912017-11-30 Virome analysis of two sympatric bat species (Desmodus rotundus and Molossus molossus) in French Guiana Salmier, Arielle Tirera, Sourakhata de Thoisy, Benoit Franc, Alain Darcissac, Edith Donato, Damien Bouchier, Christiane Lacoste, Vincent Lavergne, Anne PLoS One Research Article Environmental disturbances in the Neotropics (e.g., deforestation, agriculture intensification, urbanization) contribute to an increasing risk of cross-species transmission of microorganisms and to disease outbreaks due to changing ecosystems of reservoir hosts. Although Amazonia encompasses the greatest diversity of reservoir species, the outsized viral population diversity (virome) has yet to be investigated. Here, through a metagenomic approach, we identified 10,991 viral sequences in the saliva and feces of two bat species, Desmodus rotundus (hematophagous), trapped in two different caves surrounded by primary lowland forest, and Molossus molossus (insectivorous), trapped in forest and urban habitats. These sequences are related to 51 viral families known to infect a wide range of hosts (i.e., bacteria, plants, insects and vertebrates). Most viruses detected reflected the diet of bat species, with a high proportion of plant and insect-related viral families for M. molossus and a high proportion of vertebrate-related viral families for D. rotundus, highlighting its influence in shaping the viral diversity of bats. Lastly, we reconstructed the phylogenetic relationships for five vertebrate-related viral families (Nairoviridae, Circoviridae, Retroviridae, Herpesviridae, Papillomaviridae). The results showed highly supported clustering with other viral sequences of the same viral family hosted by other bat species, highlighting the potential association of viral diversity with the host’s diet. These findings provide significant insight into viral bat diversity in French Guiana belonging to the Amazonian biome and emphasize that habitats and the host’s dietary ecology may drive the viral diversity in the bat communities investigated. Public Library of Science 2017-11-08 /pmc/articles/PMC5695591/ /pubmed/29117243 http://dx.doi.org/10.1371/journal.pone.0186943 Text en © 2017 Salmier et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Salmier, Arielle Tirera, Sourakhata de Thoisy, Benoit Franc, Alain Darcissac, Edith Donato, Damien Bouchier, Christiane Lacoste, Vincent Lavergne, Anne Virome analysis of two sympatric bat species (Desmodus rotundus and Molossus molossus) in French Guiana |
title | Virome analysis of two sympatric bat species (Desmodus rotundus and Molossus molossus) in French Guiana |
title_full | Virome analysis of two sympatric bat species (Desmodus rotundus and Molossus molossus) in French Guiana |
title_fullStr | Virome analysis of two sympatric bat species (Desmodus rotundus and Molossus molossus) in French Guiana |
title_full_unstemmed | Virome analysis of two sympatric bat species (Desmodus rotundus and Molossus molossus) in French Guiana |
title_short | Virome analysis of two sympatric bat species (Desmodus rotundus and Molossus molossus) in French Guiana |
title_sort | virome analysis of two sympatric bat species (desmodus rotundus and molossus molossus) in french guiana |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695591/ https://www.ncbi.nlm.nih.gov/pubmed/29117243 http://dx.doi.org/10.1371/journal.pone.0186943 |
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