Cargando…
NF-κB pathways are involved in M1 polarization of RAW 264.7 macrophage by polyporus polysaccharide in the tumor microenvironment
Bladder cancer is one of the most malignant tumors closely associated with macrophages. Polyporus polysaccharide (PPS) has shown excellent efficacy in treating bladder cancer with minimal side effects. However, the molecular mechanisms underlying the effects of PPS in inhibiting bladder cancer remai...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695768/ https://www.ncbi.nlm.nih.gov/pubmed/29155869 http://dx.doi.org/10.1371/journal.pone.0188317 |
_version_ | 1783280355915071488 |
---|---|
author | Liu, Chun-Ping Zhang, Xian Tan, Qing-Long Xu, Wen-Xing Zhou, Chang-Yuan Luo, Min Li, Xiong Huang, Run-Yue Zeng, Xing |
author_facet | Liu, Chun-Ping Zhang, Xian Tan, Qing-Long Xu, Wen-Xing Zhou, Chang-Yuan Luo, Min Li, Xiong Huang, Run-Yue Zeng, Xing |
author_sort | Liu, Chun-Ping |
collection | PubMed |
description | Bladder cancer is one of the most malignant tumors closely associated with macrophages. Polyporus polysaccharide (PPS) has shown excellent efficacy in treating bladder cancer with minimal side effects. However, the molecular mechanisms underlying the effects of PPS in inhibiting bladder cancer remain unclear. In this study, we used macrophages cultured alone or with T24 human bladder cancer cell culture supernatant as study models. We found that PPS enhanced the activities of IFN-γ-stimulated RAW 264.7 macrophages, as shown by the release of inducible nitric oxide synthase (INOS), secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-6, phagocytosis activity, as well as expression of M1 phenotype indicators, such as CD40, CD284 and CD86. PPS acted upstream in activation cascade of nuclear factor (NF)-κB signaling pathways by interfering with IκB phosphorylation. In addition, PPS regulated NF-κB (P65) signaling by interfering with Toll-like receptor (TLR)-4, INOS and cyclooxygenase (COX)-2. Our results indicate that PPS activates macrophages through TLR4/NF-κB signaling pathways. |
format | Online Article Text |
id | pubmed-5695768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56957682017-11-30 NF-κB pathways are involved in M1 polarization of RAW 264.7 macrophage by polyporus polysaccharide in the tumor microenvironment Liu, Chun-Ping Zhang, Xian Tan, Qing-Long Xu, Wen-Xing Zhou, Chang-Yuan Luo, Min Li, Xiong Huang, Run-Yue Zeng, Xing PLoS One Research Article Bladder cancer is one of the most malignant tumors closely associated with macrophages. Polyporus polysaccharide (PPS) has shown excellent efficacy in treating bladder cancer with minimal side effects. However, the molecular mechanisms underlying the effects of PPS in inhibiting bladder cancer remain unclear. In this study, we used macrophages cultured alone or with T24 human bladder cancer cell culture supernatant as study models. We found that PPS enhanced the activities of IFN-γ-stimulated RAW 264.7 macrophages, as shown by the release of inducible nitric oxide synthase (INOS), secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-6, phagocytosis activity, as well as expression of M1 phenotype indicators, such as CD40, CD284 and CD86. PPS acted upstream in activation cascade of nuclear factor (NF)-κB signaling pathways by interfering with IκB phosphorylation. In addition, PPS regulated NF-κB (P65) signaling by interfering with Toll-like receptor (TLR)-4, INOS and cyclooxygenase (COX)-2. Our results indicate that PPS activates macrophages through TLR4/NF-κB signaling pathways. Public Library of Science 2017-11-20 /pmc/articles/PMC5695768/ /pubmed/29155869 http://dx.doi.org/10.1371/journal.pone.0188317 Text en © 2017 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Liu, Chun-Ping Zhang, Xian Tan, Qing-Long Xu, Wen-Xing Zhou, Chang-Yuan Luo, Min Li, Xiong Huang, Run-Yue Zeng, Xing NF-κB pathways are involved in M1 polarization of RAW 264.7 macrophage by polyporus polysaccharide in the tumor microenvironment |
title | NF-κB pathways are involved in M1 polarization of RAW 264.7 macrophage by polyporus polysaccharide in the tumor microenvironment |
title_full | NF-κB pathways are involved in M1 polarization of RAW 264.7 macrophage by polyporus polysaccharide in the tumor microenvironment |
title_fullStr | NF-κB pathways are involved in M1 polarization of RAW 264.7 macrophage by polyporus polysaccharide in the tumor microenvironment |
title_full_unstemmed | NF-κB pathways are involved in M1 polarization of RAW 264.7 macrophage by polyporus polysaccharide in the tumor microenvironment |
title_short | NF-κB pathways are involved in M1 polarization of RAW 264.7 macrophage by polyporus polysaccharide in the tumor microenvironment |
title_sort | nf-κb pathways are involved in m1 polarization of raw 264.7 macrophage by polyporus polysaccharide in the tumor microenvironment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695768/ https://www.ncbi.nlm.nih.gov/pubmed/29155869 http://dx.doi.org/10.1371/journal.pone.0188317 |
work_keys_str_mv | AT liuchunping nfkbpathwaysareinvolvedinm1polarizationofraw2647macrophagebypolyporuspolysaccharideinthetumormicroenvironment AT zhangxian nfkbpathwaysareinvolvedinm1polarizationofraw2647macrophagebypolyporuspolysaccharideinthetumormicroenvironment AT tanqinglong nfkbpathwaysareinvolvedinm1polarizationofraw2647macrophagebypolyporuspolysaccharideinthetumormicroenvironment AT xuwenxing nfkbpathwaysareinvolvedinm1polarizationofraw2647macrophagebypolyporuspolysaccharideinthetumormicroenvironment AT zhouchangyuan nfkbpathwaysareinvolvedinm1polarizationofraw2647macrophagebypolyporuspolysaccharideinthetumormicroenvironment AT luomin nfkbpathwaysareinvolvedinm1polarizationofraw2647macrophagebypolyporuspolysaccharideinthetumormicroenvironment AT lixiong nfkbpathwaysareinvolvedinm1polarizationofraw2647macrophagebypolyporuspolysaccharideinthetumormicroenvironment AT huangrunyue nfkbpathwaysareinvolvedinm1polarizationofraw2647macrophagebypolyporuspolysaccharideinthetumormicroenvironment AT zengxing nfkbpathwaysareinvolvedinm1polarizationofraw2647macrophagebypolyporuspolysaccharideinthetumormicroenvironment |