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Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women
Women with dense breast tissue on mammography are at higher risk of developing breast cancer but the underlying mechanisms are not well understood. De-regulation of microRNAs (miRNAs) has been associated with the onset of breast cancer. miRNAs in the extracellular space participate in the regulation...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5696169/ https://www.ncbi.nlm.nih.gov/pubmed/29190903 http://dx.doi.org/10.18632/oncotarget.20906 |
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author | Abrahamsson, Annelie Capodanno, Alessandra Rzepecka, Anna Dabrosin, Charlotta |
author_facet | Abrahamsson, Annelie Capodanno, Alessandra Rzepecka, Anna Dabrosin, Charlotta |
author_sort | Abrahamsson, Annelie |
collection | PubMed |
description | Women with dense breast tissue on mammography are at higher risk of developing breast cancer but the underlying mechanisms are not well understood. De-regulation of microRNAs (miRNAs) has been associated with the onset of breast cancer. miRNAs in the extracellular space participate in the regulation of the local tissue microenvironment. Here, we recruited 39 healthy postmenopausal women attending their mammography-screen that were assessed having extreme dense or entirely fatty breasts (nondense). Microdialysis was performed in breast tissue and a reference catheter was inserted in abdominal subcutaneous fat for local sampling of extracellular compounds. Three miRNAs, associated with tumor suppression, miR-193b, miR-365a, and miR-452 were significantly down-regulated in dense breast tissue compared with nondense breast tissue. In addition, miR-452 exhibited significant negative correlations with several pro-inflammatory cytokines in vivo, which was confirmed in vitro by overexpression of miR-452 in breast cancer cells. No differences were found of miR-21, -29a, -30c, 146a, -148a, -203, or -451 in breast tissue and no miRs were different in plasma. Extracellular miRNAs may be among factors that should be included in studies of novel prevention strategies for breast cancer. |
format | Online Article Text |
id | pubmed-5696169 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56961692017-11-29 Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women Abrahamsson, Annelie Capodanno, Alessandra Rzepecka, Anna Dabrosin, Charlotta Oncotarget Research Paper Women with dense breast tissue on mammography are at higher risk of developing breast cancer but the underlying mechanisms are not well understood. De-regulation of microRNAs (miRNAs) has been associated with the onset of breast cancer. miRNAs in the extracellular space participate in the regulation of the local tissue microenvironment. Here, we recruited 39 healthy postmenopausal women attending their mammography-screen that were assessed having extreme dense or entirely fatty breasts (nondense). Microdialysis was performed in breast tissue and a reference catheter was inserted in abdominal subcutaneous fat for local sampling of extracellular compounds. Three miRNAs, associated with tumor suppression, miR-193b, miR-365a, and miR-452 were significantly down-regulated in dense breast tissue compared with nondense breast tissue. In addition, miR-452 exhibited significant negative correlations with several pro-inflammatory cytokines in vivo, which was confirmed in vitro by overexpression of miR-452 in breast cancer cells. No differences were found of miR-21, -29a, -30c, 146a, -148a, -203, or -451 in breast tissue and no miRs were different in plasma. Extracellular miRNAs may be among factors that should be included in studies of novel prevention strategies for breast cancer. Impact Journals LLC 2017-09-15 /pmc/articles/PMC5696169/ /pubmed/29190903 http://dx.doi.org/10.18632/oncotarget.20906 Text en Copyright: © 2017 Abrahamsson et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Abrahamsson, Annelie Capodanno, Alessandra Rzepecka, Anna Dabrosin, Charlotta Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women |
title | Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women |
title_full | Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women |
title_fullStr | Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women |
title_full_unstemmed | Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women |
title_short | Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women |
title_sort | downregulation of tumor suppressive micrornas in vivo in dense breast tissue of postmenopausal women |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5696169/ https://www.ncbi.nlm.nih.gov/pubmed/29190903 http://dx.doi.org/10.18632/oncotarget.20906 |
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