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Programmable DNA looping using engineered bivalent dCas9 complexes

DNA looping is a ubiquitous and critical feature of gene regulation. Although DNA looping can be efficiently detected, tools to readily manipulate DNA looping are limited. Here we develop CRISPR-based DNA looping reagents for creation of programmable DNA loops. Cleavage-defective Cas9 proteins of di...

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Detalles Bibliográficos
Autores principales:	Hao, Nan, Shearwin, Keith E., Dodd, Ian B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5696343/ https://www.ncbi.nlm.nih.gov/pubmed/29158476 http://dx.doi.org/10.1038/s41467-017-01873-x

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