Photoacoustic stimulation promotes the osteogenic differentiation of bone mesenchymal stem cells to enhance the repair of bone defect

The aim of this study was to evaluate the direct photoacoustic (PA) effect on bone marrow mesenchymal stem cells (BMSCs) which is a key cell source for osteogenesis. As scaffold is also an indispensable element for tissue regeneration, here we firstly fabricated a composited sheet using polylactic-c...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Zebin, Xu, Jiankun, Chen, Jiebin, Chen, Hongjiang, Wang, Hailong, Huang, Zhonglian, Chen, Youbin, Lu, Xiaolin, Lu, Fushen, Hu, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5696557/
https://www.ncbi.nlm.nih.gov/pubmed/29158525
http://dx.doi.org/10.1038/s41598-017-15879-4
_version_ 1783280475110899712
author Huang, Zebin
Xu, Jiankun
Chen, Jiebin
Chen, Hongjiang
Wang, Hailong
Huang, Zhonglian
Chen, Youbin
Lu, Xiaolin
Lu, Fushen
Hu, Jun
author_facet Huang, Zebin
Xu, Jiankun
Chen, Jiebin
Chen, Hongjiang
Wang, Hailong
Huang, Zhonglian
Chen, Youbin
Lu, Xiaolin
Lu, Fushen
Hu, Jun
author_sort Huang, Zebin
collection PubMed
description The aim of this study was to evaluate the direct photoacoustic (PA) effect on bone marrow mesenchymal stem cells (BMSCs) which is a key cell source for osteogenesis. As scaffold is also an indispensable element for tissue regeneration, here we firstly fabricated a composited sheet using polylactic-co-glycolic acid (PLGA) mixing with graphene oxide (GO). BMSCs were seeded on the PLGA-GO sheets and received PA treatment in vitro for 3, 9 and 15 days, respectively. Then the BMSCs were harvested and subjected to assess alkaline phosphatase (ALP) activity, calcium content and osteopontin (OPN) on 3, 9 and 15 days. For in vivo study, PLGA-GO sheet seeded with BMSCs after in vitro PA stimulation for 9 days were implanted to repair the bone defect established in the femoral mid-shaft of Sprague-Dawley rat. PLGA-GO group with PA pretreatment showed promising outcomes in terms of the expression of ALP, OPN, and calcium content, thus enhanced the repair of bone defect. In conclusion, we have developed an alternative approach to enhance the repair of bone defect by making good use of the beneficial effect of PA.
format Online
Article
Text
id pubmed-5696557
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-56965572017-11-29 Photoacoustic stimulation promotes the osteogenic differentiation of bone mesenchymal stem cells to enhance the repair of bone defect Huang, Zebin Xu, Jiankun Chen, Jiebin Chen, Hongjiang Wang, Hailong Huang, Zhonglian Chen, Youbin Lu, Xiaolin Lu, Fushen Hu, Jun Sci Rep Article The aim of this study was to evaluate the direct photoacoustic (PA) effect on bone marrow mesenchymal stem cells (BMSCs) which is a key cell source for osteogenesis. As scaffold is also an indispensable element for tissue regeneration, here we firstly fabricated a composited sheet using polylactic-co-glycolic acid (PLGA) mixing with graphene oxide (GO). BMSCs were seeded on the PLGA-GO sheets and received PA treatment in vitro for 3, 9 and 15 days, respectively. Then the BMSCs were harvested and subjected to assess alkaline phosphatase (ALP) activity, calcium content and osteopontin (OPN) on 3, 9 and 15 days. For in vivo study, PLGA-GO sheet seeded with BMSCs after in vitro PA stimulation for 9 days were implanted to repair the bone defect established in the femoral mid-shaft of Sprague-Dawley rat. PLGA-GO group with PA pretreatment showed promising outcomes in terms of the expression of ALP, OPN, and calcium content, thus enhanced the repair of bone defect. In conclusion, we have developed an alternative approach to enhance the repair of bone defect by making good use of the beneficial effect of PA. Nature Publishing Group UK 2017-11-20 /pmc/articles/PMC5696557/ /pubmed/29158525 http://dx.doi.org/10.1038/s41598-017-15879-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Huang, Zebin
Xu, Jiankun
Chen, Jiebin
Chen, Hongjiang
Wang, Hailong
Huang, Zhonglian
Chen, Youbin
Lu, Xiaolin
Lu, Fushen
Hu, Jun
Photoacoustic stimulation promotes the osteogenic differentiation of bone mesenchymal stem cells to enhance the repair of bone defect
title Photoacoustic stimulation promotes the osteogenic differentiation of bone mesenchymal stem cells to enhance the repair of bone defect
title_full Photoacoustic stimulation promotes the osteogenic differentiation of bone mesenchymal stem cells to enhance the repair of bone defect
title_fullStr Photoacoustic stimulation promotes the osteogenic differentiation of bone mesenchymal stem cells to enhance the repair of bone defect
title_full_unstemmed Photoacoustic stimulation promotes the osteogenic differentiation of bone mesenchymal stem cells to enhance the repair of bone defect
title_short Photoacoustic stimulation promotes the osteogenic differentiation of bone mesenchymal stem cells to enhance the repair of bone defect
title_sort photoacoustic stimulation promotes the osteogenic differentiation of bone mesenchymal stem cells to enhance the repair of bone defect
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5696557/
https://www.ncbi.nlm.nih.gov/pubmed/29158525
http://dx.doi.org/10.1038/s41598-017-15879-4
work_keys_str_mv AT huangzebin photoacousticstimulationpromotestheosteogenicdifferentiationofbonemesenchymalstemcellstoenhancetherepairofbonedefect
AT xujiankun photoacousticstimulationpromotestheosteogenicdifferentiationofbonemesenchymalstemcellstoenhancetherepairofbonedefect
AT chenjiebin photoacousticstimulationpromotestheosteogenicdifferentiationofbonemesenchymalstemcellstoenhancetherepairofbonedefect
AT chenhongjiang photoacousticstimulationpromotestheosteogenicdifferentiationofbonemesenchymalstemcellstoenhancetherepairofbonedefect
AT wanghailong photoacousticstimulationpromotestheosteogenicdifferentiationofbonemesenchymalstemcellstoenhancetherepairofbonedefect
AT huangzhonglian photoacousticstimulationpromotestheosteogenicdifferentiationofbonemesenchymalstemcellstoenhancetherepairofbonedefect
AT chenyoubin photoacousticstimulationpromotestheosteogenicdifferentiationofbonemesenchymalstemcellstoenhancetherepairofbonedefect
AT luxiaolin photoacousticstimulationpromotestheosteogenicdifferentiationofbonemesenchymalstemcellstoenhancetherepairofbonedefect
AT lufushen photoacousticstimulationpromotestheosteogenicdifferentiationofbonemesenchymalstemcellstoenhancetherepairofbonedefect
AT hujun photoacousticstimulationpromotestheosteogenicdifferentiationofbonemesenchymalstemcellstoenhancetherepairofbonedefect

Ejemplares similares