Absence of nuclease activity in commonly used oxygen-scavenging systems

OBJECTIVE: Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in the...

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Detalles Bibliográficos
Autores principales: Gahlon, Hailey L., Poudevigne-Durance, Paul, Rueda, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5697020/
https://www.ncbi.nlm.nih.gov/pubmed/29162131
http://dx.doi.org/10.1186/s13104-017-2929-6
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author Gahlon, Hailey L.
Poudevigne-Durance, Paul
Rueda, David
author_facet Gahlon, Hailey L.
Poudevigne-Durance, Paul
Rueda, David
author_sort Gahlon, Hailey L.
collection PubMed
description OBJECTIVE: Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in these oxygen scavenging systems. RESULTS: Linear and plasmid DNA was incubated with two different oxygen scavenging systems (1) protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD) and (2) glucose-coupled glucose oxidase/catalase (GODCAT). No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems.
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spelling pubmed-56970202017-12-01 Absence of nuclease activity in commonly used oxygen-scavenging systems Gahlon, Hailey L. Poudevigne-Durance, Paul Rueda, David BMC Res Notes Research Note OBJECTIVE: Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in these oxygen scavenging systems. RESULTS: Linear and plasmid DNA was incubated with two different oxygen scavenging systems (1) protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD) and (2) glucose-coupled glucose oxidase/catalase (GODCAT). No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems. BioMed Central 2017-11-21 /pmc/articles/PMC5697020/ /pubmed/29162131 http://dx.doi.org/10.1186/s13104-017-2929-6 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Gahlon, Hailey L.
Poudevigne-Durance, Paul
Rueda, David
Absence of nuclease activity in commonly used oxygen-scavenging systems
title Absence of nuclease activity in commonly used oxygen-scavenging systems
title_full Absence of nuclease activity in commonly used oxygen-scavenging systems
title_fullStr Absence of nuclease activity in commonly used oxygen-scavenging systems
title_full_unstemmed Absence of nuclease activity in commonly used oxygen-scavenging systems
title_short Absence of nuclease activity in commonly used oxygen-scavenging systems
title_sort absence of nuclease activity in commonly used oxygen-scavenging systems
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5697020/
https://www.ncbi.nlm.nih.gov/pubmed/29162131
http://dx.doi.org/10.1186/s13104-017-2929-6
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