Cargando…

Adjuvant Therapy with Silibinin Improves the Efficacy of Paclitaxel and Cisplatin in MCF-7 Breast Cancer Cells

Herbal-derived medicines have introduced as sources of novel drugs due to minimum systemic side effects. Silibinin as a flavonoid compound has showed with effective chemotherapeutic effects on different cancers. Here, we investigated the impact of combination therapy of silibinin, with paclitaxel an...

Descripción completa

Detalles Bibliográficos
Autores principales: Chavoshi, Hadi, Vahedian, Vahid, Saghaei, Somaiyeh, Pirouzpanah, Mohammad Bagher, Raeisi, Mortaza, Samadi, Nasser
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5697488/
https://www.ncbi.nlm.nih.gov/pubmed/28843263
http://dx.doi.org/10.22034/APJCP.2017.18.8.2243
_version_ 1783280634130595840
author Chavoshi, Hadi
Vahedian, Vahid
Saghaei, Somaiyeh
Pirouzpanah, Mohammad Bagher
Raeisi, Mortaza
Samadi, Nasser
author_facet Chavoshi, Hadi
Vahedian, Vahid
Saghaei, Somaiyeh
Pirouzpanah, Mohammad Bagher
Raeisi, Mortaza
Samadi, Nasser
author_sort Chavoshi, Hadi
collection PubMed
description Herbal-derived medicines have introduced as sources of novel drugs due to minimum systemic side effects. Silibinin as a flavonoid compound has showed with effective chemotherapeutic effects on different cancers. Here, we investigated the impact of combination therapy of silibinin, with paclitaxel and cisplatin in inhibition of proliferation and induction of apoptosis in MCF-7 cells. Cell proliferation was assessed by MTT assay and the percentage of apoptotic cells was measured using flowcytometric assay. Understand of molecular mechanism of this combination related to apoptotic pathway were evaluated by Real Time RT-PCR assays. The IC(50) values for silibinin, paclitaxel and cisplatin were 160 ± 22.2 µM, 33.7 ± 4.2 nM and 3.2 ± 0.5 µM, respectively. Paclitaxel and cisplatin induced higher percentage of apoptosis in MCF-7 (P < 0.05). Treatment of cell line with combination of silibinin and paclitaxel or cisplatin showed enhanced early apoptosis 56% and 61%, respectively (P < 0.05). Gene expression patterns demonstrated a significant decrease in anti-apoptotic Bcl-2 with increase in pro-apoptotic Bax, P53, BRCA1 and ATM mRNA levels. Taken together combination therapy of breast cancer cells by applying paclitaxel or cisplatin with silibinin synergistically increases the anti-proliferative effect of single agents.
format Online
Article
Text
id pubmed-5697488
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher West Asia Organization for Cancer Prevention
record_format MEDLINE/PubMed
spelling pubmed-56974882017-12-01 Adjuvant Therapy with Silibinin Improves the Efficacy of Paclitaxel and Cisplatin in MCF-7 Breast Cancer Cells Chavoshi, Hadi Vahedian, Vahid Saghaei, Somaiyeh Pirouzpanah, Mohammad Bagher Raeisi, Mortaza Samadi, Nasser Asian Pac J Cancer Prev Research Article Herbal-derived medicines have introduced as sources of novel drugs due to minimum systemic side effects. Silibinin as a flavonoid compound has showed with effective chemotherapeutic effects on different cancers. Here, we investigated the impact of combination therapy of silibinin, with paclitaxel and cisplatin in inhibition of proliferation and induction of apoptosis in MCF-7 cells. Cell proliferation was assessed by MTT assay and the percentage of apoptotic cells was measured using flowcytometric assay. Understand of molecular mechanism of this combination related to apoptotic pathway were evaluated by Real Time RT-PCR assays. The IC(50) values for silibinin, paclitaxel and cisplatin were 160 ± 22.2 µM, 33.7 ± 4.2 nM and 3.2 ± 0.5 µM, respectively. Paclitaxel and cisplatin induced higher percentage of apoptosis in MCF-7 (P < 0.05). Treatment of cell line with combination of silibinin and paclitaxel or cisplatin showed enhanced early apoptosis 56% and 61%, respectively (P < 0.05). Gene expression patterns demonstrated a significant decrease in anti-apoptotic Bcl-2 with increase in pro-apoptotic Bax, P53, BRCA1 and ATM mRNA levels. Taken together combination therapy of breast cancer cells by applying paclitaxel or cisplatin with silibinin synergistically increases the anti-proliferative effect of single agents. West Asia Organization for Cancer Prevention 2017 /pmc/articles/PMC5697488/ /pubmed/28843263 http://dx.doi.org/10.22034/APJCP.2017.18.8.2243 Text en Copyright: © Asian Pacific Journal of Cancer Prevention http://creativecommons.org/licenses/BY-SA/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Research Article
Chavoshi, Hadi
Vahedian, Vahid
Saghaei, Somaiyeh
Pirouzpanah, Mohammad Bagher
Raeisi, Mortaza
Samadi, Nasser
Adjuvant Therapy with Silibinin Improves the Efficacy of Paclitaxel and Cisplatin in MCF-7 Breast Cancer Cells
title Adjuvant Therapy with Silibinin Improves the Efficacy of Paclitaxel and Cisplatin in MCF-7 Breast Cancer Cells
title_full Adjuvant Therapy with Silibinin Improves the Efficacy of Paclitaxel and Cisplatin in MCF-7 Breast Cancer Cells
title_fullStr Adjuvant Therapy with Silibinin Improves the Efficacy of Paclitaxel and Cisplatin in MCF-7 Breast Cancer Cells
title_full_unstemmed Adjuvant Therapy with Silibinin Improves the Efficacy of Paclitaxel and Cisplatin in MCF-7 Breast Cancer Cells
title_short Adjuvant Therapy with Silibinin Improves the Efficacy of Paclitaxel and Cisplatin in MCF-7 Breast Cancer Cells
title_sort adjuvant therapy with silibinin improves the efficacy of paclitaxel and cisplatin in mcf-7 breast cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5697488/
https://www.ncbi.nlm.nih.gov/pubmed/28843263
http://dx.doi.org/10.22034/APJCP.2017.18.8.2243
work_keys_str_mv AT chavoshihadi adjuvanttherapywithsilibininimprovestheefficacyofpaclitaxelandcisplatininmcf7breastcancercells
AT vahedianvahid adjuvanttherapywithsilibininimprovestheefficacyofpaclitaxelandcisplatininmcf7breastcancercells
AT saghaeisomaiyeh adjuvanttherapywithsilibininimprovestheefficacyofpaclitaxelandcisplatininmcf7breastcancercells
AT pirouzpanahmohammadbagher adjuvanttherapywithsilibininimprovestheefficacyofpaclitaxelandcisplatininmcf7breastcancercells
AT raeisimortaza adjuvanttherapywithsilibininimprovestheefficacyofpaclitaxelandcisplatininmcf7breastcancercells
AT samadinasser adjuvanttherapywithsilibininimprovestheefficacyofpaclitaxelandcisplatininmcf7breastcancercells