Precise mass determination of single cell with cantilever-based microbiosensor system

Having determined the mass of a single cell of brewer yeast Saccharomyces cerevisiae by means of a microcantilever-based biosensor Cantisens CSR-801 (Concentris, Basel, Switzerland), it was found that its dry mass is 47,65 ± 1,05 pg. Found to be crucial in this mass determination was the cell positi...

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Detalles Bibliográficos
Autores principales: Łabędź, Bogdan, Wańczyk, Aleksandra, Rajfur, Zenon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5697875/
https://www.ncbi.nlm.nih.gov/pubmed/29161333
http://dx.doi.org/10.1371/journal.pone.0188388
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author Łabędź, Bogdan
Wańczyk, Aleksandra
Rajfur, Zenon
author_facet Łabędź, Bogdan
Wańczyk, Aleksandra
Rajfur, Zenon
author_sort Łabędź, Bogdan
collection PubMed
description Having determined the mass of a single cell of brewer yeast Saccharomyces cerevisiae by means of a microcantilever-based biosensor Cantisens CSR-801 (Concentris, Basel, Switzerland), it was found that its dry mass is 47,65 ± 1,05 pg. Found to be crucial in this mass determination was the cell position along the length of the cantilever. Moreover, calculations including cells positions on the cantilever provide a threefold better degree of accuracy than those which assume uniform mass distribution. We have also examined the influence of storage time on the single cell mass. Our results show that after 6 months there is an increase in the average mass of a single yeast cell.
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spelling pubmed-56978752017-11-30 Precise mass determination of single cell with cantilever-based microbiosensor system Łabędź, Bogdan Wańczyk, Aleksandra Rajfur, Zenon PLoS One Research Article Having determined the mass of a single cell of brewer yeast Saccharomyces cerevisiae by means of a microcantilever-based biosensor Cantisens CSR-801 (Concentris, Basel, Switzerland), it was found that its dry mass is 47,65 ± 1,05 pg. Found to be crucial in this mass determination was the cell position along the length of the cantilever. Moreover, calculations including cells positions on the cantilever provide a threefold better degree of accuracy than those which assume uniform mass distribution. We have also examined the influence of storage time on the single cell mass. Our results show that after 6 months there is an increase in the average mass of a single yeast cell. Public Library of Science 2017-11-21 /pmc/articles/PMC5697875/ /pubmed/29161333 http://dx.doi.org/10.1371/journal.pone.0188388 Text en © 2017 Łabędź et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Łabędź, Bogdan
Wańczyk, Aleksandra
Rajfur, Zenon
Precise mass determination of single cell with cantilever-based microbiosensor system
title Precise mass determination of single cell with cantilever-based microbiosensor system
title_full Precise mass determination of single cell with cantilever-based microbiosensor system
title_fullStr Precise mass determination of single cell with cantilever-based microbiosensor system
title_full_unstemmed Precise mass determination of single cell with cantilever-based microbiosensor system
title_short Precise mass determination of single cell with cantilever-based microbiosensor system
title_sort precise mass determination of single cell with cantilever-based microbiosensor system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5697875/
https://www.ncbi.nlm.nih.gov/pubmed/29161333
http://dx.doi.org/10.1371/journal.pone.0188388
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