Cargando…

Direct PCR Offers a Fast and Reliable Alternative to Conventional DNA Isolation Methods for Gut Microbiomes

The gut microbiome of animals is emerging as an important factor influencing ecological and evolutionary processes. A major bottleneck in obtaining microbiome data from large numbers of samples is the time-consuming laboratory procedures required, specifically the isolation of DNA and generation of...

Descripción completa

Detalles Bibliográficos
Autores principales:	Videvall, Elin, Strandh, Maria, Engelbrecht, Anel, Cloete, Schalk, Cornwallis, Charlie K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2017
Materias:	Methods and Protocols
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5698494/ https://www.ncbi.nlm.nih.gov/pubmed/29181448 http://dx.doi.org/10.1128/mSystems.00132-17

Ejemplares similares

Coprophagy rapidly matures juvenile gut microbiota in a precocial bird
por: Videvall, Elin, et al.
Publicado: (2023)

Early-life gut dysbiosis linked to juvenile mortality in ostriches
por: Videvall, Elin, et al.
Publicado: (2020)

Evolutionary trade-offs between heat and cold tolerance limit responses to fluctuating climates
por: Schou, Mads F., et al.
Publicado: (2022)

Experimental evidence that group size generates divergent benefits of cooperative breeding for male and female ostriches
por: Melgar, Julian, et al.
Publicado: (2022)

Extreme temperatures compromise male and female fertility in a large desert bird
por: Schou, Mads F., et al.
Publicado: (2021)

The Avian Transcriptome Response to Malaria Infection
por: Videvall, Elin, et al.
Publicado: (2015)

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
por: Whale, Alexandra S., et al.
Publicado: (2012)

The evolutionary maintenance of ancient recombining sex chromosomes in the ostrich
por: Yazdi, Homa Papoli, et al.
Publicado: (2023)

Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
por: Marullo, Manuela, et al.
Publicado: (2010)

Detection of alternative lengthening of telomeres by telomere quantitative PCR
por: Lau, Loretta M. S., et al.
Publicado: (2013)

The Effect of Extensive Human Presence at an Early Age on Stress Responses and Reactivity of Juvenile Ostriches towards Humans
por: Muvhali, Pfunzo T., et al.
Publicado: (2018)

Evaluation of two new highly multiplexed PCR assays as an alternative to next‐generation sequencing for IDH1/2 mutation detection
por: Favre, Loetitia, et al.
Publicado: (2022)

Quantifying the benefit offered by transcript assembly with Scallop-LR on single-molecule long reads
por: Tung, Laura H., et al.
Publicado: (2019)

Processing Method Affects Oral and Gut Microbiome Composition
por: Superdock, Dorothy, et al.
Publicado: (2022)

Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes
por: Neuzil, Pavel, et al.
Publicado: (2006)

Leveraging explainable AI for gut microbiome-based colorectal cancer classification
por: Rynazal, Ryza, et al.
Publicado: (2023)

Plasmodium parasites of birds have the most AT-rich genes of eukaryotes
por: Videvall, Elin
Publicado: (2018)

CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity
por: Carrington, Blake, et al.
Publicado: (2015)

Is Phylotranscriptomics as Reliable as Phylogenomics?
por: Cheon, Seongmin, et al.
Publicado: (2020)

The Alteration of the Gut Microbiome during Ramadan Offers a Novel Perspective on Ramadan Fasting: A Pilot Study
por: Jo, YoungJae, et al.
Publicado: (2023)

Namco: a microbiome explorer
por: Dietrich, Alexander, et al.
Publicado: (2022)

Advantages of q-PCR as a method of screening for gene targeting in mammalian cells using conventional and whole BAC-based constructs
por: Gómez-Rodríguez, Julio, et al.
Publicado: (2008)

Fast, inexpensive, and reliable HPLC method to determine monomer fractions in poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
por: Duvigneau, Stefanie, et al.
Publicado: (2021)

An adaptive association test for microbiome data
por: Wu, Chong, et al.
Publicado: (2016)

Morphological analysis of the filamentous fungus Penicillium chrysogenum using flow cytometry—the fast alternative to microscopic image analysis
por: Ehgartner, Daniela, et al.
Publicado: (2017)

Phylogenomic Subsampling and the Search for Phylogenetically Reliable Loci
por: Mongiardino Koch, Nicolás
Publicado: (2021)

Detection of Klebsiella pneumoniae human gut carriage: a comparison of culture, qPCR, and whole metagenomic sequencing methods
por: Lindstedt, Kenneth, et al.
Publicado: (2022)

Development of SCAR markers for rapid and specific detection of Pseudomonas syringae pv. morsprunorum races 1 and 2, using conventional and real-time PCR
por: Kałużna, Monika, et al.
Publicado: (2016)

A rapid and simple quantitative method for specific detection of smaller coterminal RNA by PCR (DeSCo-PCR): application to the detection of viral subgenomic RNAs
por: Kanodia, Pulkit, et al.
Publicado: (2020)

Palidis: fast discovery of novel insertion sequences
por: Carr, Victoria R., et al.
Publicado: (2023)

Osseous Healing With Non-Rigid Suture Fixation Offers a Compelling Alternative to Conventional Latarjet Procedure
por: Kalia, Vivek, et al.
Publicado: (2019)

Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
por: Aird, Daniel, et al.
Publicado: (2011)

Sonification as a reliable alternative to conventional visual surgical navigation
por: Matinfar, Sasan, et al.
Publicado: (2023)

A simple, fast, and accurate method of phylogenomic inference
por: Wu, Martin, et al.
Publicado: (2008)

Fast and scalable inference of multi-sample cancer lineages
por: Popic, Victoria, et al.
Publicado: (2015)

Fast and flexible bacterial genomic epidemiology with PopPUNK
por: Lees, John A., et al.
Publicado: (2019)

Reliable measurements of brain atrophy in individual patients with multiple sclerosis
por: Smeets, Dirk, et al.
Publicado: (2016)

Efficient computation of Faith's phylogenetic diversity with applications in characterizing microbiomes
por: Armstrong, George, et al.
Publicado: (2021)

mbImpute: an accurate and robust imputation method for microbiome data
por: Jiang, Ruochen, et al.
Publicado: (2021)

Determining the impact of uncharacterized inversions in the human genome by droplet digital PCR
por: Puig, Marta, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_5e6sl0146ogt8673j888apan1i