Cargando…
Recombination rate plasticity: revealing mechanisms by design
For over a century, scientists have known that meiotic recombination rates can vary considerably among individuals, and that environmental conditions can modify recombination rates relative to the background. A variety of external and intrinsic factors such as temperature, age, sex and starvation ca...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5698621/ https://www.ncbi.nlm.nih.gov/pubmed/29109222 http://dx.doi.org/10.1098/rstb.2016.0459 |
_version_ | 1783280796396683264 |
---|---|
author | Stevison, Laurie S. Sefick, Stephen Rushton, Chase Graze, Rita M. |
author_facet | Stevison, Laurie S. Sefick, Stephen Rushton, Chase Graze, Rita M. |
author_sort | Stevison, Laurie S. |
collection | PubMed |
description | For over a century, scientists have known that meiotic recombination rates can vary considerably among individuals, and that environmental conditions can modify recombination rates relative to the background. A variety of external and intrinsic factors such as temperature, age, sex and starvation can elicit ‘plastic’ responses in recombination rate. The influence of recombination rate plasticity on genetic diversity of the next generation has interesting and important implications for how populations evolve. Further, many questions remain regarding the mechanisms and molecular processes that contribute to recombination rate plasticity. Here, we review 100 years of experimental work on recombination rate plasticity conducted in Drosophila melanogaster. We categorize this work into four major classes of experimental designs, which we describe via classic studies in D. melanogaster. Based on these studies, we highlight molecular mechanisms that are supported by experimental results and relate these findings to studies in other systems. We synthesize lessons learned from this model system into experimental guidelines for using recent advances in genotyping technologies, to study recombination rate plasticity in non-model organisms. Specifically, we recommend (1) using fine-scale genome-wide markers, (2) collecting time-course data, (3) including crossover distribution measurements, and (4) using mixed effects models to analyse results. To illustrate this approach, we present an application adhering to these guidelines from empirical work we conducted in Drosophila pseudoobscura. This article is part of the themed issue ‘Evolutionary causes and consequences of recombination rate variation in sexual organisms’. |
format | Online Article Text |
id | pubmed-5698621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-56986212017-11-29 Recombination rate plasticity: revealing mechanisms by design Stevison, Laurie S. Sefick, Stephen Rushton, Chase Graze, Rita M. Philos Trans R Soc Lond B Biol Sci Articles For over a century, scientists have known that meiotic recombination rates can vary considerably among individuals, and that environmental conditions can modify recombination rates relative to the background. A variety of external and intrinsic factors such as temperature, age, sex and starvation can elicit ‘plastic’ responses in recombination rate. The influence of recombination rate plasticity on genetic diversity of the next generation has interesting and important implications for how populations evolve. Further, many questions remain regarding the mechanisms and molecular processes that contribute to recombination rate plasticity. Here, we review 100 years of experimental work on recombination rate plasticity conducted in Drosophila melanogaster. We categorize this work into four major classes of experimental designs, which we describe via classic studies in D. melanogaster. Based on these studies, we highlight molecular mechanisms that are supported by experimental results and relate these findings to studies in other systems. We synthesize lessons learned from this model system into experimental guidelines for using recent advances in genotyping technologies, to study recombination rate plasticity in non-model organisms. Specifically, we recommend (1) using fine-scale genome-wide markers, (2) collecting time-course data, (3) including crossover distribution measurements, and (4) using mixed effects models to analyse results. To illustrate this approach, we present an application adhering to these guidelines from empirical work we conducted in Drosophila pseudoobscura. This article is part of the themed issue ‘Evolutionary causes and consequences of recombination rate variation in sexual organisms’. The Royal Society 2017-12-19 2017-11-06 /pmc/articles/PMC5698621/ /pubmed/29109222 http://dx.doi.org/10.1098/rstb.2016.0459 Text en © 2017 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Articles Stevison, Laurie S. Sefick, Stephen Rushton, Chase Graze, Rita M. Recombination rate plasticity: revealing mechanisms by design |
title | Recombination rate plasticity: revealing mechanisms by design |
title_full | Recombination rate plasticity: revealing mechanisms by design |
title_fullStr | Recombination rate plasticity: revealing mechanisms by design |
title_full_unstemmed | Recombination rate plasticity: revealing mechanisms by design |
title_short | Recombination rate plasticity: revealing mechanisms by design |
title_sort | recombination rate plasticity: revealing mechanisms by design |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5698621/ https://www.ncbi.nlm.nih.gov/pubmed/29109222 http://dx.doi.org/10.1098/rstb.2016.0459 |
work_keys_str_mv | AT stevisonlauries recombinationrateplasticityrevealingmechanismsbydesign AT sefickstephen recombinationrateplasticityrevealingmechanismsbydesign AT rushtonchase recombinationrateplasticityrevealingmechanismsbydesign AT grazeritam recombinationrateplasticityrevealingmechanismsbydesign |