Metabolic engineering of Schizosaccharomyces pombe via CRISPR-Cas9 genome editing for lactic acid production from glucose and cellobiose

Modification of the Schizosaccharomyces pombe genome is often laborious, time consuming due to the lower efficiency of homologous recombination. Here, we constructed metabolically engineered S. pombe strains using a CRISPR-Cas9 system and also demonstrated D-lactic acid (D-LA) production from glucos...

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Detalles Bibliográficos
Autores principales: Ozaki, Aiko, Konishi, Rie, Otomo, Chisako, Kishida, Mayumi, Takayama, Seiya, Matsumoto, Takuya, Tanaka, Tsutomu, Kondo, Akihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5699526/
https://www.ncbi.nlm.nih.gov/pubmed/29188185
http://dx.doi.org/10.1016/j.meteno.2017.08.002

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