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Improvements to parallel plate flow chambers to reduce reagent and cellular requirements
BACKGROUND: The parallel plate flow chamber has become a mainstay for examination of leukocytes under physiologic flow conditions. Several design modifications have occurred over the years, yet a comparison of these different designs has not been performed. In addition, the reagent requirements of m...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC56996/ https://www.ncbi.nlm.nih.gov/pubmed/11580861 http://dx.doi.org/10.1186/1471-2172-2-9 |
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author | Brown, David C Larson, Richard S |
author_facet | Brown, David C Larson, Richard S |
author_sort | Brown, David C |
collection | PubMed |
description | BACKGROUND: The parallel plate flow chamber has become a mainstay for examination of leukocytes under physiologic flow conditions. Several design modifications have occurred over the years, yet a comparison of these different designs has not been performed. In addition, the reagent requirements of many designs prohibit the study of rare leukocyte populations and require large amounts of reagents. RESULTS: In this study, we evaluate modifications to a newer parallel plate flow chamber design in comparison to the original parallel plate flow chamber described by Lawrence et al. We show that modifications in the chamber size, internal tubing diameters, injection valves, and a recirculation design may dramatically reduce the cellular and reagent requirements without altering measurements. CONCLUSIONS: These modifications are simple and easily implemented so that study of rare leukocyte subsets using scarce or expensive reagents can occur. |
format | Text |
id | pubmed-56996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-569962001-10-02 Improvements to parallel plate flow chambers to reduce reagent and cellular requirements Brown, David C Larson, Richard S BMC Immunol Methodology Article BACKGROUND: The parallel plate flow chamber has become a mainstay for examination of leukocytes under physiologic flow conditions. Several design modifications have occurred over the years, yet a comparison of these different designs has not been performed. In addition, the reagent requirements of many designs prohibit the study of rare leukocyte populations and require large amounts of reagents. RESULTS: In this study, we evaluate modifications to a newer parallel plate flow chamber design in comparison to the original parallel plate flow chamber described by Lawrence et al. We show that modifications in the chamber size, internal tubing diameters, injection valves, and a recirculation design may dramatically reduce the cellular and reagent requirements without altering measurements. CONCLUSIONS: These modifications are simple and easily implemented so that study of rare leukocyte subsets using scarce or expensive reagents can occur. BioMed Central 2001-09-11 /pmc/articles/PMC56996/ /pubmed/11580861 http://dx.doi.org/10.1186/1471-2172-2-9 Text en Copyright © 2001 Brown and Larson; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
spellingShingle | Methodology Article Brown, David C Larson, Richard S Improvements to parallel plate flow chambers to reduce reagent and cellular requirements |
title | Improvements to parallel plate flow chambers to reduce reagent and cellular requirements |
title_full | Improvements to parallel plate flow chambers to reduce reagent and cellular requirements |
title_fullStr | Improvements to parallel plate flow chambers to reduce reagent and cellular requirements |
title_full_unstemmed | Improvements to parallel plate flow chambers to reduce reagent and cellular requirements |
title_short | Improvements to parallel plate flow chambers to reduce reagent and cellular requirements |
title_sort | improvements to parallel plate flow chambers to reduce reagent and cellular requirements |
topic | Methodology Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC56996/ https://www.ncbi.nlm.nih.gov/pubmed/11580861 http://dx.doi.org/10.1186/1471-2172-2-9 |
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