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Improvements to parallel plate flow chambers to reduce reagent and cellular requirements

BACKGROUND: The parallel plate flow chamber has become a mainstay for examination of leukocytes under physiologic flow conditions. Several design modifications have occurred over the years, yet a comparison of these different designs has not been performed. In addition, the reagent requirements of m...

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Detalles Bibliográficos
Autores principales: Brown, David C, Larson, Richard S
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC56996/
https://www.ncbi.nlm.nih.gov/pubmed/11580861
http://dx.doi.org/10.1186/1471-2172-2-9
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author Brown, David C
Larson, Richard S
author_facet Brown, David C
Larson, Richard S
author_sort Brown, David C
collection PubMed
description BACKGROUND: The parallel plate flow chamber has become a mainstay for examination of leukocytes under physiologic flow conditions. Several design modifications have occurred over the years, yet a comparison of these different designs has not been performed. In addition, the reagent requirements of many designs prohibit the study of rare leukocyte populations and require large amounts of reagents. RESULTS: In this study, we evaluate modifications to a newer parallel plate flow chamber design in comparison to the original parallel plate flow chamber described by Lawrence et al. We show that modifications in the chamber size, internal tubing diameters, injection valves, and a recirculation design may dramatically reduce the cellular and reagent requirements without altering measurements. CONCLUSIONS: These modifications are simple and easily implemented so that study of rare leukocyte subsets using scarce or expensive reagents can occur.
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spelling pubmed-569962001-10-02 Improvements to parallel plate flow chambers to reduce reagent and cellular requirements Brown, David C Larson, Richard S BMC Immunol Methodology Article BACKGROUND: The parallel plate flow chamber has become a mainstay for examination of leukocytes under physiologic flow conditions. Several design modifications have occurred over the years, yet a comparison of these different designs has not been performed. In addition, the reagent requirements of many designs prohibit the study of rare leukocyte populations and require large amounts of reagents. RESULTS: In this study, we evaluate modifications to a newer parallel plate flow chamber design in comparison to the original parallel plate flow chamber described by Lawrence et al. We show that modifications in the chamber size, internal tubing diameters, injection valves, and a recirculation design may dramatically reduce the cellular and reagent requirements without altering measurements. CONCLUSIONS: These modifications are simple and easily implemented so that study of rare leukocyte subsets using scarce or expensive reagents can occur. BioMed Central 2001-09-11 /pmc/articles/PMC56996/ /pubmed/11580861 http://dx.doi.org/10.1186/1471-2172-2-9 Text en Copyright © 2001 Brown and Larson; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Methodology Article
Brown, David C
Larson, Richard S
Improvements to parallel plate flow chambers to reduce reagent and cellular requirements
title Improvements to parallel plate flow chambers to reduce reagent and cellular requirements
title_full Improvements to parallel plate flow chambers to reduce reagent and cellular requirements
title_fullStr Improvements to parallel plate flow chambers to reduce reagent and cellular requirements
title_full_unstemmed Improvements to parallel plate flow chambers to reduce reagent and cellular requirements
title_short Improvements to parallel plate flow chambers to reduce reagent and cellular requirements
title_sort improvements to parallel plate flow chambers to reduce reagent and cellular requirements
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC56996/
https://www.ncbi.nlm.nih.gov/pubmed/11580861
http://dx.doi.org/10.1186/1471-2172-2-9
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