ChrA(SO), the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction

The chromate efflux pump encoding gene chrA(SO) was identified on the chromosome of Shewanella oneidensis MR1. Although chrA(SO) is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrA(SO) exhibits a chromate sensitive phenotype co...

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Detalles Bibliográficos
Autores principales: Baaziz, Hiba, Gambari, Cyril, Boyeldieu, Anne, Ali Chaouche, Amine, Alatou, Radia, Méjean, Vincent, Jourlin-Castelli, Cécile, Fons, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5699817/
https://www.ncbi.nlm.nih.gov/pubmed/29166414
http://dx.doi.org/10.1371/journal.pone.0188516
Descripción
Sumario:The chromate efflux pump encoding gene chrA(SO) was identified on the chromosome of Shewanella oneidensis MR1. Although chrA(SO) is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrA(SO) exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrA(SO) in E. coli confers resistance to high chromate concentration. Moreover, expression of chrA(SO) in S. oneidensis and E. coli significantly improves Cr(VI) reduction. This effect could result either from extracytoplasmic chromate reduction or from a better cell survival leading to enhanced Cr(VI) reduction.

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