ChrA(SO), the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction

The chromate efflux pump encoding gene chrA(SO) was identified on the chromosome of Shewanella oneidensis MR1. Although chrA(SO) is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrA(SO) exhibits a chromate sensitive phenotype co...

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Autores principales: Baaziz, Hiba, Gambari, Cyril, Boyeldieu, Anne, Ali Chaouche, Amine, Alatou, Radia, Méjean, Vincent, Jourlin-Castelli, Cécile, Fons, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5699817/
https://www.ncbi.nlm.nih.gov/pubmed/29166414
http://dx.doi.org/10.1371/journal.pone.0188516
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author Baaziz, Hiba
Gambari, Cyril
Boyeldieu, Anne
Ali Chaouche, Amine
Alatou, Radia
Méjean, Vincent
Jourlin-Castelli, Cécile
Fons, Michel
author_facet Baaziz, Hiba
Gambari, Cyril
Boyeldieu, Anne
Ali Chaouche, Amine
Alatou, Radia
Méjean, Vincent
Jourlin-Castelli, Cécile
Fons, Michel
author_sort Baaziz, Hiba
collection PubMed
description The chromate efflux pump encoding gene chrA(SO) was identified on the chromosome of Shewanella oneidensis MR1. Although chrA(SO) is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrA(SO) exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrA(SO) in E. coli confers resistance to high chromate concentration. Moreover, expression of chrA(SO) in S. oneidensis and E. coli significantly improves Cr(VI) reduction. This effect could result either from extracytoplasmic chromate reduction or from a better cell survival leading to enhanced Cr(VI) reduction.
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spelling pubmed-56998172017-12-08 ChrA(SO), the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction Baaziz, Hiba Gambari, Cyril Boyeldieu, Anne Ali Chaouche, Amine Alatou, Radia Méjean, Vincent Jourlin-Castelli, Cécile Fons, Michel PLoS One Research Article The chromate efflux pump encoding gene chrA(SO) was identified on the chromosome of Shewanella oneidensis MR1. Although chrA(SO) is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrA(SO) exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrA(SO) in E. coli confers resistance to high chromate concentration. Moreover, expression of chrA(SO) in S. oneidensis and E. coli significantly improves Cr(VI) reduction. This effect could result either from extracytoplasmic chromate reduction or from a better cell survival leading to enhanced Cr(VI) reduction. Public Library of Science 2017-11-22 /pmc/articles/PMC5699817/ /pubmed/29166414 http://dx.doi.org/10.1371/journal.pone.0188516 Text en © 2017 Baaziz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Baaziz, Hiba
Gambari, Cyril
Boyeldieu, Anne
Ali Chaouche, Amine
Alatou, Radia
Méjean, Vincent
Jourlin-Castelli, Cécile
Fons, Michel
ChrA(SO), the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction
title ChrA(SO), the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction
title_full ChrA(SO), the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction
title_fullStr ChrA(SO), the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction
title_full_unstemmed ChrA(SO), the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction
title_short ChrA(SO), the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction
title_sort chra(so), the chromate efflux pump of shewanella oneidensis, improves chromate survival and reduction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5699817/
https://www.ncbi.nlm.nih.gov/pubmed/29166414
http://dx.doi.org/10.1371/journal.pone.0188516
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