Cargando…
E3 ubiquitin ligase Bre1 couples sister chromatid cohesion establishment to DNA replication in Saccharomyces cerevisiae
Bre1, a conserved E3 ubiquitin ligase in Saccharomyces cerevisiae, together with its interacting partner Lge1, are responsible for histone H2B monoubiquitination, which regulates transcription, DNA replication, and DNA damage response and repair, ensuring the structural integrity of the genome. Dele...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5699866/ https://www.ncbi.nlm.nih.gov/pubmed/29058668 http://dx.doi.org/10.7554/eLife.28231 |
_version_ | 1783281033074966528 |
---|---|
author | Zhang, Wei Yeung, Clarence Hue Lok Wu, Liwen Yuen, Karen Wing Yee |
author_facet | Zhang, Wei Yeung, Clarence Hue Lok Wu, Liwen Yuen, Karen Wing Yee |
author_sort | Zhang, Wei |
collection | PubMed |
description | Bre1, a conserved E3 ubiquitin ligase in Saccharomyces cerevisiae, together with its interacting partner Lge1, are responsible for histone H2B monoubiquitination, which regulates transcription, DNA replication, and DNA damage response and repair, ensuring the structural integrity of the genome. Deletion of BRE1 or LGE1 also results in whole chromosome instability. We discovered a novel role for Bre1, Lge1 and H2Bub1 in chromosome segregation and sister chromatid cohesion. Bre1’s function in G1 and S phases contributes to cohesion establishment, but it is not required for cohesion maintenance in G2 phase. Bre1 is dispensable for the loading of cohesin complex to chromatin in G1, but regulates the localization of replication factor Mcm10 and cohesion establishment factors Ctf4, Ctf18 and Eco1 to early replication origins in G1 and S phases, and promotes cohesin subunit Smc3 acetylation for cohesion stabilization. H2Bub1 epigenetically marks the origins, potentially signaling the coupling of DNA replication and cohesion establishment. |
format | Online Article Text |
id | pubmed-5699866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-56998662017-11-24 E3 ubiquitin ligase Bre1 couples sister chromatid cohesion establishment to DNA replication in Saccharomyces cerevisiae Zhang, Wei Yeung, Clarence Hue Lok Wu, Liwen Yuen, Karen Wing Yee eLife Cell Biology Bre1, a conserved E3 ubiquitin ligase in Saccharomyces cerevisiae, together with its interacting partner Lge1, are responsible for histone H2B monoubiquitination, which regulates transcription, DNA replication, and DNA damage response and repair, ensuring the structural integrity of the genome. Deletion of BRE1 or LGE1 also results in whole chromosome instability. We discovered a novel role for Bre1, Lge1 and H2Bub1 in chromosome segregation and sister chromatid cohesion. Bre1’s function in G1 and S phases contributes to cohesion establishment, but it is not required for cohesion maintenance in G2 phase. Bre1 is dispensable for the loading of cohesin complex to chromatin in G1, but regulates the localization of replication factor Mcm10 and cohesion establishment factors Ctf4, Ctf18 and Eco1 to early replication origins in G1 and S phases, and promotes cohesin subunit Smc3 acetylation for cohesion stabilization. H2Bub1 epigenetically marks the origins, potentially signaling the coupling of DNA replication and cohesion establishment. eLife Sciences Publications, Ltd 2017-10-23 /pmc/articles/PMC5699866/ /pubmed/29058668 http://dx.doi.org/10.7554/eLife.28231 Text en © 2017, Zhang et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Zhang, Wei Yeung, Clarence Hue Lok Wu, Liwen Yuen, Karen Wing Yee E3 ubiquitin ligase Bre1 couples sister chromatid cohesion establishment to DNA replication in Saccharomyces cerevisiae |
title | E3 ubiquitin ligase Bre1 couples sister chromatid cohesion establishment to DNA replication in Saccharomyces cerevisiae |
title_full | E3 ubiquitin ligase Bre1 couples sister chromatid cohesion establishment to DNA replication in Saccharomyces cerevisiae |
title_fullStr | E3 ubiquitin ligase Bre1 couples sister chromatid cohesion establishment to DNA replication in Saccharomyces cerevisiae |
title_full_unstemmed | E3 ubiquitin ligase Bre1 couples sister chromatid cohesion establishment to DNA replication in Saccharomyces cerevisiae |
title_short | E3 ubiquitin ligase Bre1 couples sister chromatid cohesion establishment to DNA replication in Saccharomyces cerevisiae |
title_sort | e3 ubiquitin ligase bre1 couples sister chromatid cohesion establishment to dna replication in saccharomyces cerevisiae |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5699866/ https://www.ncbi.nlm.nih.gov/pubmed/29058668 http://dx.doi.org/10.7554/eLife.28231 |
work_keys_str_mv | AT zhangwei e3ubiquitinligasebre1couplessisterchromatidcohesionestablishmenttodnareplicationinsaccharomycescerevisiae AT yeungclarencehuelok e3ubiquitinligasebre1couplessisterchromatidcohesionestablishmenttodnareplicationinsaccharomycescerevisiae AT wuliwen e3ubiquitinligasebre1couplessisterchromatidcohesionestablishmenttodnareplicationinsaccharomycescerevisiae AT yuenkarenwingyee e3ubiquitinligasebre1couplessisterchromatidcohesionestablishmenttodnareplicationinsaccharomycescerevisiae |