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Insights into the species-specific metabolic engineering of glucosinolates in radish (Raphanus sativus L.) based on comparative genomic analysis

Glucosinolates (GSLs) and their hydrolysis products present in Brassicales play important roles in plants against herbivores and pathogens as well as in the protection of human health. To elucidate the molecular mechanisms underlying the formation of species-specific GSLs and their hydrolysed produc...

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Autores principales: Wang, Jinglei, Qiu, Yang, Wang, Xiaowu, Yue, Zhen, Yang, Xinhua, Chen, Xiaohua, Zhang, Xiaohui, Shen, Di, Wang, Haiping, Song, Jiangping, He, Hongju, Li, Xixiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700054/
https://www.ncbi.nlm.nih.gov/pubmed/29167500
http://dx.doi.org/10.1038/s41598-017-16306-4
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author Wang, Jinglei
Qiu, Yang
Wang, Xiaowu
Yue, Zhen
Yang, Xinhua
Chen, Xiaohua
Zhang, Xiaohui
Shen, Di
Wang, Haiping
Song, Jiangping
He, Hongju
Li, Xixiang
author_facet Wang, Jinglei
Qiu, Yang
Wang, Xiaowu
Yue, Zhen
Yang, Xinhua
Chen, Xiaohua
Zhang, Xiaohui
Shen, Di
Wang, Haiping
Song, Jiangping
He, Hongju
Li, Xixiang
author_sort Wang, Jinglei
collection PubMed
description Glucosinolates (GSLs) and their hydrolysis products present in Brassicales play important roles in plants against herbivores and pathogens as well as in the protection of human health. To elucidate the molecular mechanisms underlying the formation of species-specific GSLs and their hydrolysed products in Raphanus sativus L., we performed a comparative genomics analysis between R. sativus and Arabidopsis thaliana. In total, 144 GSL metabolism genes were identified, and most of these GSL genes have expanded through whole-genome and tandem duplication in R. sativus. Crucially, the differential expression of FMOGS-OX2 in the root and silique correlates with the differential distribution of major aliphatic GSL components in these organs. Moreover, MYB118 expression specifically in the silique suggests that aliphatic GSL accumulation occurs predominantly in seeds. Furthermore, the absence of the expression of a putative non-functional epithiospecifier (ESP) gene in any tissue and the nitrile-specifier (NSP) gene in roots facilitates the accumulation of distinctive beneficial isothiocyanates in R. sativus. Elucidating the evolution of the GSL metabolic pathway in R. sativus is important for fully understanding GSL metabolic engineering and the precise genetic improvement of GSL components and their catabolites in R. sativus and other Brassicaceae crops.
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spelling pubmed-57000542017-11-30 Insights into the species-specific metabolic engineering of glucosinolates in radish (Raphanus sativus L.) based on comparative genomic analysis Wang, Jinglei Qiu, Yang Wang, Xiaowu Yue, Zhen Yang, Xinhua Chen, Xiaohua Zhang, Xiaohui Shen, Di Wang, Haiping Song, Jiangping He, Hongju Li, Xixiang Sci Rep Article Glucosinolates (GSLs) and their hydrolysis products present in Brassicales play important roles in plants against herbivores and pathogens as well as in the protection of human health. To elucidate the molecular mechanisms underlying the formation of species-specific GSLs and their hydrolysed products in Raphanus sativus L., we performed a comparative genomics analysis between R. sativus and Arabidopsis thaliana. In total, 144 GSL metabolism genes were identified, and most of these GSL genes have expanded through whole-genome and tandem duplication in R. sativus. Crucially, the differential expression of FMOGS-OX2 in the root and silique correlates with the differential distribution of major aliphatic GSL components in these organs. Moreover, MYB118 expression specifically in the silique suggests that aliphatic GSL accumulation occurs predominantly in seeds. Furthermore, the absence of the expression of a putative non-functional epithiospecifier (ESP) gene in any tissue and the nitrile-specifier (NSP) gene in roots facilitates the accumulation of distinctive beneficial isothiocyanates in R. sativus. Elucidating the evolution of the GSL metabolic pathway in R. sativus is important for fully understanding GSL metabolic engineering and the precise genetic improvement of GSL components and their catabolites in R. sativus and other Brassicaceae crops. Nature Publishing Group UK 2017-11-22 /pmc/articles/PMC5700054/ /pubmed/29167500 http://dx.doi.org/10.1038/s41598-017-16306-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Jinglei
Qiu, Yang
Wang, Xiaowu
Yue, Zhen
Yang, Xinhua
Chen, Xiaohua
Zhang, Xiaohui
Shen, Di
Wang, Haiping
Song, Jiangping
He, Hongju
Li, Xixiang
Insights into the species-specific metabolic engineering of glucosinolates in radish (Raphanus sativus L.) based on comparative genomic analysis
title Insights into the species-specific metabolic engineering of glucosinolates in radish (Raphanus sativus L.) based on comparative genomic analysis
title_full Insights into the species-specific metabolic engineering of glucosinolates in radish (Raphanus sativus L.) based on comparative genomic analysis
title_fullStr Insights into the species-specific metabolic engineering of glucosinolates in radish (Raphanus sativus L.) based on comparative genomic analysis
title_full_unstemmed Insights into the species-specific metabolic engineering of glucosinolates in radish (Raphanus sativus L.) based on comparative genomic analysis
title_short Insights into the species-specific metabolic engineering of glucosinolates in radish (Raphanus sativus L.) based on comparative genomic analysis
title_sort insights into the species-specific metabolic engineering of glucosinolates in radish (raphanus sativus l.) based on comparative genomic analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700054/
https://www.ncbi.nlm.nih.gov/pubmed/29167500
http://dx.doi.org/10.1038/s41598-017-16306-4
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