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Computational Lipidomics of the Neuronal Plasma Membrane
Membrane lipid composition varies greatly within submembrane compartments, different organelle membranes, and also between cells of different cell stage, cell and tissue types, and organisms. Environmental factors (such as diet) also influence membrane composition. The membrane lipid composition is...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700369/ https://www.ncbi.nlm.nih.gov/pubmed/29113676 http://dx.doi.org/10.1016/j.bpj.2017.10.017 |
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author | Ingólfsson, Helgi I. Carpenter, Timothy S. Bhatia, Harsh Bremer, Peer-Timo Marrink, Siewert J. Lightstone, Felice C. |
author_facet | Ingólfsson, Helgi I. Carpenter, Timothy S. Bhatia, Harsh Bremer, Peer-Timo Marrink, Siewert J. Lightstone, Felice C. |
author_sort | Ingólfsson, Helgi I. |
collection | PubMed |
description | Membrane lipid composition varies greatly within submembrane compartments, different organelle membranes, and also between cells of different cell stage, cell and tissue types, and organisms. Environmental factors (such as diet) also influence membrane composition. The membrane lipid composition is tightly regulated by the cell, maintaining a homeostasis that, if disrupted, can impair cell function and lead to disease. This is especially pronounced in the brain, where defects in lipid regulation are linked to various neurological diseases. The tightly regulated diversity raises questions on how complex changes in composition affect overall bilayer properties, dynamics, and lipid organization of cellular membranes. Here, we utilize recent advances in computational power and molecular dynamics force fields to develop and test a realistically complex human brain plasma membrane (PM) lipid model and extend previous work on an idealized, “average” mammalian PM. The PMs showed both striking similarities, despite significantly different lipid composition, and interesting differences. The main differences in composition (higher cholesterol concentration and increased tail unsaturation in brain PM) appear to have opposite, yet complementary, influences on many bilayer properties. Both mixtures exhibit a range of dynamic lipid lateral inhomogeneities (“domains”). The domains can be small and transient or larger and more persistent and can correlate between the leaflets depending on lipid mixture, Brain or Average, as well as on the extent of bilayer undulations. |
format | Online Article Text |
id | pubmed-5700369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Biophysical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-57003692018-11-21 Computational Lipidomics of the Neuronal Plasma Membrane Ingólfsson, Helgi I. Carpenter, Timothy S. Bhatia, Harsh Bremer, Peer-Timo Marrink, Siewert J. Lightstone, Felice C. Biophys J Articles Membrane lipid composition varies greatly within submembrane compartments, different organelle membranes, and also between cells of different cell stage, cell and tissue types, and organisms. Environmental factors (such as diet) also influence membrane composition. The membrane lipid composition is tightly regulated by the cell, maintaining a homeostasis that, if disrupted, can impair cell function and lead to disease. This is especially pronounced in the brain, where defects in lipid regulation are linked to various neurological diseases. The tightly regulated diversity raises questions on how complex changes in composition affect overall bilayer properties, dynamics, and lipid organization of cellular membranes. Here, we utilize recent advances in computational power and molecular dynamics force fields to develop and test a realistically complex human brain plasma membrane (PM) lipid model and extend previous work on an idealized, “average” mammalian PM. The PMs showed both striking similarities, despite significantly different lipid composition, and interesting differences. The main differences in composition (higher cholesterol concentration and increased tail unsaturation in brain PM) appear to have opposite, yet complementary, influences on many bilayer properties. Both mixtures exhibit a range of dynamic lipid lateral inhomogeneities (“domains”). The domains can be small and transient or larger and more persistent and can correlate between the leaflets depending on lipid mixture, Brain or Average, as well as on the extent of bilayer undulations. The Biophysical Society 2017-11-21 2017-11-04 /pmc/articles/PMC5700369/ /pubmed/29113676 http://dx.doi.org/10.1016/j.bpj.2017.10.017 Text en © 2017 Biophysical Society. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Articles Ingólfsson, Helgi I. Carpenter, Timothy S. Bhatia, Harsh Bremer, Peer-Timo Marrink, Siewert J. Lightstone, Felice C. Computational Lipidomics of the Neuronal Plasma Membrane |
title | Computational Lipidomics of the Neuronal Plasma Membrane |
title_full | Computational Lipidomics of the Neuronal Plasma Membrane |
title_fullStr | Computational Lipidomics of the Neuronal Plasma Membrane |
title_full_unstemmed | Computational Lipidomics of the Neuronal Plasma Membrane |
title_short | Computational Lipidomics of the Neuronal Plasma Membrane |
title_sort | computational lipidomics of the neuronal plasma membrane |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700369/ https://www.ncbi.nlm.nih.gov/pubmed/29113676 http://dx.doi.org/10.1016/j.bpj.2017.10.017 |
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