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Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation
The transmission of malaria parasites to mosquitoes relies on the rapid induction of sexual reproduction upon their ingestion into a blood meal. Haploid female and male gametocytes become activated and emerge from their host cells, and the males enter the cell cycle to produce eight microgametes. Th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700370/ https://www.ncbi.nlm.nih.gov/pubmed/29141230 http://dx.doi.org/10.1016/j.celrep.2017.10.071 |
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author | Invergo, Brandon M. Brochet, Mathieu Yu, Lu Choudhary, Jyoti Beltrao, Pedro Billker, Oliver |
author_facet | Invergo, Brandon M. Brochet, Mathieu Yu, Lu Choudhary, Jyoti Beltrao, Pedro Billker, Oliver |
author_sort | Invergo, Brandon M. |
collection | PubMed |
description | The transmission of malaria parasites to mosquitoes relies on the rapid induction of sexual reproduction upon their ingestion into a blood meal. Haploid female and male gametocytes become activated and emerge from their host cells, and the males enter the cell cycle to produce eight microgametes. The synchronized nature of gametogenesis allowed us to investigate phosphorylation signaling during its first minute in Plasmodium berghei via a high-resolution time course of the phosphoproteome. This revealed an unexpectedly broad response, with proteins related to distinct cell cycle events undergoing simultaneous phosphoregulation. We implicate several protein kinases in the process, and we validate our analyses on the plant-like calcium-dependent protein kinase 4 (CDPK4) and a homolog of serine/arginine-rich protein kinases (SRPK1). Mutants in these kinases displayed distinct phosphoproteomic disruptions, consistent with differences in their phenotypes. The results reveal the central role of protein phosphorylation in the atypical cell cycle regulation of a divergent eukaryote. |
format | Online Article Text |
id | pubmed-5700370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-57003702017-12-01 Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation Invergo, Brandon M. Brochet, Mathieu Yu, Lu Choudhary, Jyoti Beltrao, Pedro Billker, Oliver Cell Rep Article The transmission of malaria parasites to mosquitoes relies on the rapid induction of sexual reproduction upon their ingestion into a blood meal. Haploid female and male gametocytes become activated and emerge from their host cells, and the males enter the cell cycle to produce eight microgametes. The synchronized nature of gametogenesis allowed us to investigate phosphorylation signaling during its first minute in Plasmodium berghei via a high-resolution time course of the phosphoproteome. This revealed an unexpectedly broad response, with proteins related to distinct cell cycle events undergoing simultaneous phosphoregulation. We implicate several protein kinases in the process, and we validate our analyses on the plant-like calcium-dependent protein kinase 4 (CDPK4) and a homolog of serine/arginine-rich protein kinases (SRPK1). Mutants in these kinases displayed distinct phosphoproteomic disruptions, consistent with differences in their phenotypes. The results reveal the central role of protein phosphorylation in the atypical cell cycle regulation of a divergent eukaryote. Cell Press 2017-11-14 /pmc/articles/PMC5700370/ /pubmed/29141230 http://dx.doi.org/10.1016/j.celrep.2017.10.071 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Invergo, Brandon M. Brochet, Mathieu Yu, Lu Choudhary, Jyoti Beltrao, Pedro Billker, Oliver Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation |
title | Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation |
title_full | Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation |
title_fullStr | Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation |
title_full_unstemmed | Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation |
title_short | Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation |
title_sort | sub-minute phosphoregulation of cell cycle systems during plasmodium gamete formation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700370/ https://www.ncbi.nlm.nih.gov/pubmed/29141230 http://dx.doi.org/10.1016/j.celrep.2017.10.071 |
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