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Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes

BACKGROUND: The pathogenesis of age-related macular degeneration (AMD) is complex. It has been shown that vitreomacular traction (VMT) plays a role in the pathogenesis of AMD. We speculate that the continuous stretch induced by VMT might impair the function of retinal pigment epithelium (RPE) cells...

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Autores principales: Wu, Shen, Lu, Qingjun, Wang, Ningli, Zhang, Jingxue, Liu, Qian, Gao, Meng, Chen, Jinqiu, Liu, Wu, Xu, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700533/
https://www.ncbi.nlm.nih.gov/pubmed/29166888
http://dx.doi.org/10.1186/s12886-017-0606-0
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author Wu, Shen
Lu, Qingjun
Wang, Ningli
Zhang, Jingxue
Liu, Qian
Gao, Meng
Chen, Jinqiu
Liu, Wu
Xu, Liang
author_facet Wu, Shen
Lu, Qingjun
Wang, Ningli
Zhang, Jingxue
Liu, Qian
Gao, Meng
Chen, Jinqiu
Liu, Wu
Xu, Liang
author_sort Wu, Shen
collection PubMed
description BACKGROUND: The pathogenesis of age-related macular degeneration (AMD) is complex. It has been shown that vitreomacular traction (VMT) plays a role in the pathogenesis of AMD. We speculate that the continuous stretch induced by VMT might impair the function of retinal pigment epithelium (RPE) cells and it might also be involved in the progression of AMD. METHODS: Cultured ARPE-19 cells were subjected to cyclic stretch on the Flexcell Strain system at a level of 25% increment on the surface area for 8 h, 14 h, 20 h, 24 h. In another group, the stretch was withdrawn at 14 h and the cell cultured for another 6 h. Then, we observed the changes in morphology, apoptosis and expression of interleukin 6 (IL6) and vascular endothelial growth factor (VEGF) in RPE cells under stretch. RESULTS: We found that stretch induced the RPE cells to change from a spreading shape into a rounded shape, and that the morphological changes were positively correlated with the duration of the stretch. The expression of pFAK397 and pRac1/cdc42 were elevated in a time-dependent fashion. The stretch resulted in an increase in the apoptosis ratio, with Bcl2, Bax and p53 also showing time-dependent changes. In addition, up-regulation of IL6 and VEGF expression levels was also observed. After withdrawal of the stretch, all of these changes were significantly diminished. CONCLUSION: Stretch may induce morphological, cell apoptosis, and up-regulation of cytokines changes in RPE cells, indicating that cyclic stretching may participate in the progression of AMD by impeding the functions of the RPE.
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spelling pubmed-57005332017-12-01 Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes Wu, Shen Lu, Qingjun Wang, Ningli Zhang, Jingxue Liu, Qian Gao, Meng Chen, Jinqiu Liu, Wu Xu, Liang BMC Ophthalmol Research Article BACKGROUND: The pathogenesis of age-related macular degeneration (AMD) is complex. It has been shown that vitreomacular traction (VMT) plays a role in the pathogenesis of AMD. We speculate that the continuous stretch induced by VMT might impair the function of retinal pigment epithelium (RPE) cells and it might also be involved in the progression of AMD. METHODS: Cultured ARPE-19 cells were subjected to cyclic stretch on the Flexcell Strain system at a level of 25% increment on the surface area for 8 h, 14 h, 20 h, 24 h. In another group, the stretch was withdrawn at 14 h and the cell cultured for another 6 h. Then, we observed the changes in morphology, apoptosis and expression of interleukin 6 (IL6) and vascular endothelial growth factor (VEGF) in RPE cells under stretch. RESULTS: We found that stretch induced the RPE cells to change from a spreading shape into a rounded shape, and that the morphological changes were positively correlated with the duration of the stretch. The expression of pFAK397 and pRac1/cdc42 were elevated in a time-dependent fashion. The stretch resulted in an increase in the apoptosis ratio, with Bcl2, Bax and p53 also showing time-dependent changes. In addition, up-regulation of IL6 and VEGF expression levels was also observed. After withdrawal of the stretch, all of these changes were significantly diminished. CONCLUSION: Stretch may induce morphological, cell apoptosis, and up-regulation of cytokines changes in RPE cells, indicating that cyclic stretching may participate in the progression of AMD by impeding the functions of the RPE. BioMed Central 2017-11-22 /pmc/articles/PMC5700533/ /pubmed/29166888 http://dx.doi.org/10.1186/s12886-017-0606-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wu, Shen
Lu, Qingjun
Wang, Ningli
Zhang, Jingxue
Liu, Qian
Gao, Meng
Chen, Jinqiu
Liu, Wu
Xu, Liang
Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes
title Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes
title_full Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes
title_fullStr Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes
title_full_unstemmed Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes
title_short Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes
title_sort cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700533/
https://www.ncbi.nlm.nih.gov/pubmed/29166888
http://dx.doi.org/10.1186/s12886-017-0606-0
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