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The inhibitory effect of different concentrations of KH902 eye drops on corneal neovascularization induced by alkali burn

PURPOSE: The aim of this study was to evaluate the inhibitory effect of different concentrations of KH902 eye drops on rabbit corneal neovascularization (CNV) induced by alkali burn. METHODS: Forty-eight adult rabbits were randomized into four groups after alkali burning: Group A (2.5 mg/ml), Group...

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Autores principales: Wu, Yan, Xue, Chunyan, Lu, Yan, Huang, Zhenping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700579/
https://www.ncbi.nlm.nih.gov/pubmed/29133637
http://dx.doi.org/10.4103/ijo.IJO_339_17
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author Wu, Yan
Xue, Chunyan
Lu, Yan
Huang, Zhenping
author_facet Wu, Yan
Xue, Chunyan
Lu, Yan
Huang, Zhenping
author_sort Wu, Yan
collection PubMed
description PURPOSE: The aim of this study was to evaluate the inhibitory effect of different concentrations of KH902 eye drops on rabbit corneal neovascularization (CNV) induced by alkali burn. METHODS: Forty-eight adult rabbits were randomized into four groups after alkali burning: Group A (2.5 mg/ml), Group B (5 mg/ml), and Group C (10 mg/ml) by different concentrations of KH902 eye drops and Group D by saline solution as control with three times a day for 2 weeks. At days 7, 14, and 28, the anterior segment photographs, confocal microscopy, and histopathology were performed to evaluate corneal opacity, neovascularization, inflammatory cell density, vessel size, and edema. Immunohistochemistry was applied to analyze the vascular endothelial growth factor (VEGF) level. RESULTS: (1) The CNV in the medicine-treated groups showed a reduction without obvious corneal side effects histologically. (2) Compared to the control group, the three medicine-treated groups showed a reduction in the VEGF levels and CNV areas on days 7, 14, and 28 and in the inflammatory cell density on days 14 and 28 (P < 0.01). The difference of inflammatory cell density between the three medicine-treated groups existed on day 14 (P < 0.01). There were differences in the VEGF levels between Groups A, B, and C on days 7, 14, and 28 (P < 0.01), not for Groups B and C on day 28 (P > 0.05). CONCLUSION: KH902 eye drops in lower concentration showed an obvious reduction of the CNV growing for rabbit corneal alkali burn without side effects.
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spelling pubmed-57005792017-12-01 The inhibitory effect of different concentrations of KH902 eye drops on corneal neovascularization induced by alkali burn Wu, Yan Xue, Chunyan Lu, Yan Huang, Zhenping Indian J Ophthalmol Original Article PURPOSE: The aim of this study was to evaluate the inhibitory effect of different concentrations of KH902 eye drops on rabbit corneal neovascularization (CNV) induced by alkali burn. METHODS: Forty-eight adult rabbits were randomized into four groups after alkali burning: Group A (2.5 mg/ml), Group B (5 mg/ml), and Group C (10 mg/ml) by different concentrations of KH902 eye drops and Group D by saline solution as control with three times a day for 2 weeks. At days 7, 14, and 28, the anterior segment photographs, confocal microscopy, and histopathology were performed to evaluate corneal opacity, neovascularization, inflammatory cell density, vessel size, and edema. Immunohistochemistry was applied to analyze the vascular endothelial growth factor (VEGF) level. RESULTS: (1) The CNV in the medicine-treated groups showed a reduction without obvious corneal side effects histologically. (2) Compared to the control group, the three medicine-treated groups showed a reduction in the VEGF levels and CNV areas on days 7, 14, and 28 and in the inflammatory cell density on days 14 and 28 (P < 0.01). The difference of inflammatory cell density between the three medicine-treated groups existed on day 14 (P < 0.01). There were differences in the VEGF levels between Groups A, B, and C on days 7, 14, and 28 (P < 0.01), not for Groups B and C on day 28 (P > 0.05). CONCLUSION: KH902 eye drops in lower concentration showed an obvious reduction of the CNV growing for rabbit corneal alkali burn without side effects. Medknow Publications & Media Pvt Ltd 2017-11 /pmc/articles/PMC5700579/ /pubmed/29133637 http://dx.doi.org/10.4103/ijo.IJO_339_17 Text en Copyright: © 2017 Indian Journal of Ophthalmology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Wu, Yan
Xue, Chunyan
Lu, Yan
Huang, Zhenping
The inhibitory effect of different concentrations of KH902 eye drops on corneal neovascularization induced by alkali burn
title The inhibitory effect of different concentrations of KH902 eye drops on corneal neovascularization induced by alkali burn
title_full The inhibitory effect of different concentrations of KH902 eye drops on corneal neovascularization induced by alkali burn
title_fullStr The inhibitory effect of different concentrations of KH902 eye drops on corneal neovascularization induced by alkali burn
title_full_unstemmed The inhibitory effect of different concentrations of KH902 eye drops on corneal neovascularization induced by alkali burn
title_short The inhibitory effect of different concentrations of KH902 eye drops on corneal neovascularization induced by alkali burn
title_sort inhibitory effect of different concentrations of kh902 eye drops on corneal neovascularization induced by alkali burn
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700579/
https://www.ncbi.nlm.nih.gov/pubmed/29133637
http://dx.doi.org/10.4103/ijo.IJO_339_17
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