Cargando…

Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy

BACKGROUND: Parthenocarpy is an excellent agronomic trait that enables crops to set fruit in the absence of pollination and fertilization, and therefore to produce seedless fruit. Although parthenocarpy is widely recognized as a hormone-dependent process, hormone-insensitive parthenocarpy can also b...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Ji, Xu, Jian, Guo, Qin-Wei, Wu, Zhe, Zhang, Ting, Zhang, Kai-Jing, Cheng, Chun-yan, Zhu, Pin-yu, Lou, Qun-Feng, Chen, Jin-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700656/
https://www.ncbi.nlm.nih.gov/pubmed/29166853
http://dx.doi.org/10.1186/s12864-017-4290-5
_version_ 1783281170172084224
author Li, Ji
Xu, Jian
Guo, Qin-Wei
Wu, Zhe
Zhang, Ting
Zhang, Kai-Jing
Cheng, Chun-yan
Zhu, Pin-yu
Lou, Qun-Feng
Chen, Jin-Feng
author_facet Li, Ji
Xu, Jian
Guo, Qin-Wei
Wu, Zhe
Zhang, Ting
Zhang, Kai-Jing
Cheng, Chun-yan
Zhu, Pin-yu
Lou, Qun-Feng
Chen, Jin-Feng
author_sort Li, Ji
collection PubMed
description BACKGROUND: Parthenocarpy is an excellent agronomic trait that enables crops to set fruit in the absence of pollination and fertilization, and therefore to produce seedless fruit. Although parthenocarpy is widely recognized as a hormone-dependent process, hormone-insensitive parthenocarpy can also be observed in cucumber; however, its mechanism is poorly understood. To improve the global understanding of parthenocarpy and address the hormone-insensitive parthenocarpy shown in cucumber, we conducted a physiological and proteomic analysis of differently developed fruits. RESULTS: Physiological analysis indicated that the natural hormone-insensitive parthenocarpy of ‘EC1’ has broad hormone-inhibitor resistance, and the endogenous hormones in the natural parthenocarpy (NP) fruits were stable and relatively lower than those of the non-parthenocarpic cultivar ‘8419 s-1.’ Based on the iTRAQ technique, 683 fruit developmental proteins were identified from NP, cytokinin-induced parthenocarpic (CP), pollinated and unpollinated fruits. Gene Ontology (GO) analysis showed that proteins detected from both set and aborted fruits were involved in similar biological processes, such as cell growth, the cell cycle, cell death and communication. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that ‘protein synthesis’ was the major biological process that differed between fruit set and fruit abortion. Clustering analysis revealed that different protein expression patterns were involved in CP and NP fruits. Forty-one parthenocarpy-specialized DEPs (differentially expressed proteins) were screened and divided into two distinctive groups: NP-specialized proteins and CP-specialized proteins. Furthermore, qRT-PCR and western blot analysis indicated that NP-specialized proteins showed hormone- or hormone-inhibitor insensitive expression patterns in both ovaries and seedlings. CONCLUSIONS: In this study, the global molecular regulation of fruit development in cucumber was revealed at the protein level. Physiological and proteomic comparisons indicated the presence of hormone-independent parthenocarpy and suppression of fruit abortion in cucumber. The proteomic analysis suggested that hormone-independent parthenocarpy is regulated by hormone-insensitive proteins such as the NP-specialized proteins. Moreover, the regulation of fruit abortion suppression may be closely related to protein synthesis pathways. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-017-4290-5) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5700656
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-57006562017-12-01 Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy Li, Ji Xu, Jian Guo, Qin-Wei Wu, Zhe Zhang, Ting Zhang, Kai-Jing Cheng, Chun-yan Zhu, Pin-yu Lou, Qun-Feng Chen, Jin-Feng BMC Genomics Research Article BACKGROUND: Parthenocarpy is an excellent agronomic trait that enables crops to set fruit in the absence of pollination and fertilization, and therefore to produce seedless fruit. Although parthenocarpy is widely recognized as a hormone-dependent process, hormone-insensitive parthenocarpy can also be observed in cucumber; however, its mechanism is poorly understood. To improve the global understanding of parthenocarpy and address the hormone-insensitive parthenocarpy shown in cucumber, we conducted a physiological and proteomic analysis of differently developed fruits. RESULTS: Physiological analysis indicated that the natural hormone-insensitive parthenocarpy of ‘EC1’ has broad hormone-inhibitor resistance, and the endogenous hormones in the natural parthenocarpy (NP) fruits were stable and relatively lower than those of the non-parthenocarpic cultivar ‘8419 s-1.’ Based on the iTRAQ technique, 683 fruit developmental proteins were identified from NP, cytokinin-induced parthenocarpic (CP), pollinated and unpollinated fruits. Gene Ontology (GO) analysis showed that proteins detected from both set and aborted fruits were involved in similar biological processes, such as cell growth, the cell cycle, cell death and communication. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that ‘protein synthesis’ was the major biological process that differed between fruit set and fruit abortion. Clustering analysis revealed that different protein expression patterns were involved in CP and NP fruits. Forty-one parthenocarpy-specialized DEPs (differentially expressed proteins) were screened and divided into two distinctive groups: NP-specialized proteins and CP-specialized proteins. Furthermore, qRT-PCR and western blot analysis indicated that NP-specialized proteins showed hormone- or hormone-inhibitor insensitive expression patterns in both ovaries and seedlings. CONCLUSIONS: In this study, the global molecular regulation of fruit development in cucumber was revealed at the protein level. Physiological and proteomic comparisons indicated the presence of hormone-independent parthenocarpy and suppression of fruit abortion in cucumber. The proteomic analysis suggested that hormone-independent parthenocarpy is regulated by hormone-insensitive proteins such as the NP-specialized proteins. Moreover, the regulation of fruit abortion suppression may be closely related to protein synthesis pathways. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-017-4290-5) contains supplementary material, which is available to authorized users. BioMed Central 2017-11-22 /pmc/articles/PMC5700656/ /pubmed/29166853 http://dx.doi.org/10.1186/s12864-017-4290-5 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Li, Ji
Xu, Jian
Guo, Qin-Wei
Wu, Zhe
Zhang, Ting
Zhang, Kai-Jing
Cheng, Chun-yan
Zhu, Pin-yu
Lou, Qun-Feng
Chen, Jin-Feng
Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy
title Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy
title_full Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy
title_fullStr Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy
title_full_unstemmed Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy
title_short Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy
title_sort proteomic insight into fruit set of cucumber (cucumis sativus l.) suggests the cues of hormone-independent parthenocarpy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700656/
https://www.ncbi.nlm.nih.gov/pubmed/29166853
http://dx.doi.org/10.1186/s12864-017-4290-5
work_keys_str_mv AT liji proteomicinsightintofruitsetofcucumbercucumissativuslsuggeststhecuesofhormoneindependentparthenocarpy
AT xujian proteomicinsightintofruitsetofcucumbercucumissativuslsuggeststhecuesofhormoneindependentparthenocarpy
AT guoqinwei proteomicinsightintofruitsetofcucumbercucumissativuslsuggeststhecuesofhormoneindependentparthenocarpy
AT wuzhe proteomicinsightintofruitsetofcucumbercucumissativuslsuggeststhecuesofhormoneindependentparthenocarpy
AT zhangting proteomicinsightintofruitsetofcucumbercucumissativuslsuggeststhecuesofhormoneindependentparthenocarpy
AT zhangkaijing proteomicinsightintofruitsetofcucumbercucumissativuslsuggeststhecuesofhormoneindependentparthenocarpy
AT chengchunyan proteomicinsightintofruitsetofcucumbercucumissativuslsuggeststhecuesofhormoneindependentparthenocarpy
AT zhupinyu proteomicinsightintofruitsetofcucumbercucumissativuslsuggeststhecuesofhormoneindependentparthenocarpy
AT louqunfeng proteomicinsightintofruitsetofcucumbercucumissativuslsuggeststhecuesofhormoneindependentparthenocarpy
AT chenjinfeng proteomicinsightintofruitsetofcucumbercucumissativuslsuggeststhecuesofhormoneindependentparthenocarpy