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Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep
Circular RNAs (circRNAs) are a class of animal non-coding RNAs and play an impor-tant role in animal growth and development. However, the expression and function of circRNAs in the pituitary gland of sheep are unclear. Transcriptome profiling of circRNAs in the pituitary gland of sheep may enable us...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700919/ https://www.ncbi.nlm.nih.gov/pubmed/29170496 http://dx.doi.org/10.1038/s41598-017-16344-y |
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author | Li, Cunyuan Li, Xiaoyue Ma, Qiman Zhang, Xiangyu Cao, Yang Yao, Yang You, Shuang Wang, Dawei Quan, Renzhe Hou, Xiaoxu Liu, Zhijin Zhan, Qianqian Liu, Li Zhang, Mengdan Yu, Shuting Ni, Wei Hu, Shengwei |
author_facet | Li, Cunyuan Li, Xiaoyue Ma, Qiman Zhang, Xiangyu Cao, Yang Yao, Yang You, Shuang Wang, Dawei Quan, Renzhe Hou, Xiaoxu Liu, Zhijin Zhan, Qianqian Liu, Li Zhang, Mengdan Yu, Shuting Ni, Wei Hu, Shengwei |
author_sort | Li, Cunyuan |
collection | PubMed |
description | Circular RNAs (circRNAs) are a class of animal non-coding RNAs and play an impor-tant role in animal growth and development. However, the expression and function of circRNAs in the pituitary gland of sheep are unclear. Transcriptome profiling of circRNAs in the pituitary gland of sheep may enable us to understand their biological functions. In the present study, we identified 10,226 circRNAs from RNA-seq data in the pituitary gland of prenatal and postnatal sheep. Reverse transcription PCR and DNA sequencing analysis confirmed the presence of several circRNAs. Real-time RT-PCR analysis showed that sheep circRNAs are resistant to RNase R digestion and are expressed in prenatal and postnatal pituitary glands. GO and KEGG enrichment analysis showed that host genes of differentially expressed circRNAs are involved in the regulation of hormone secretion as well as in several pathways related to these processes. We determined that numerous circRNAs interact with pituitary-specific miRNAs that are involved in the biologic functions of the pituitary gland. Moreover, several circRNAs contain at least one IRES element and open reading frame, indicating their potential to encode proteins. Our study provides comprehensive expression profiles of circRNAs in the pituitary gland, thereby offering a valuable resource for circRNA biology in sheep. |
format | Online Article Text |
id | pubmed-5700919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57009192017-11-30 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep Li, Cunyuan Li, Xiaoyue Ma, Qiman Zhang, Xiangyu Cao, Yang Yao, Yang You, Shuang Wang, Dawei Quan, Renzhe Hou, Xiaoxu Liu, Zhijin Zhan, Qianqian Liu, Li Zhang, Mengdan Yu, Shuting Ni, Wei Hu, Shengwei Sci Rep Article Circular RNAs (circRNAs) are a class of animal non-coding RNAs and play an impor-tant role in animal growth and development. However, the expression and function of circRNAs in the pituitary gland of sheep are unclear. Transcriptome profiling of circRNAs in the pituitary gland of sheep may enable us to understand their biological functions. In the present study, we identified 10,226 circRNAs from RNA-seq data in the pituitary gland of prenatal and postnatal sheep. Reverse transcription PCR and DNA sequencing analysis confirmed the presence of several circRNAs. Real-time RT-PCR analysis showed that sheep circRNAs are resistant to RNase R digestion and are expressed in prenatal and postnatal pituitary glands. GO and KEGG enrichment analysis showed that host genes of differentially expressed circRNAs are involved in the regulation of hormone secretion as well as in several pathways related to these processes. We determined that numerous circRNAs interact with pituitary-specific miRNAs that are involved in the biologic functions of the pituitary gland. Moreover, several circRNAs contain at least one IRES element and open reading frame, indicating their potential to encode proteins. Our study provides comprehensive expression profiles of circRNAs in the pituitary gland, thereby offering a valuable resource for circRNA biology in sheep. Nature Publishing Group UK 2017-11-23 /pmc/articles/PMC5700919/ /pubmed/29170496 http://dx.doi.org/10.1038/s41598-017-16344-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Li, Cunyuan Li, Xiaoyue Ma, Qiman Zhang, Xiangyu Cao, Yang Yao, Yang You, Shuang Wang, Dawei Quan, Renzhe Hou, Xiaoxu Liu, Zhijin Zhan, Qianqian Liu, Li Zhang, Mengdan Yu, Shuting Ni, Wei Hu, Shengwei Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep |
title | Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep |
title_full | Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep |
title_fullStr | Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep |
title_full_unstemmed | Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep |
title_short | Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep |
title_sort | genome-wide analysis of circular rnas in prenatal and postnatal pituitary glands of sheep |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700919/ https://www.ncbi.nlm.nih.gov/pubmed/29170496 http://dx.doi.org/10.1038/s41598-017-16344-y |
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