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Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein

Human fibroblast growth factor 21 (hFGF21) has been characterized as an important regulator of glucose and lipid metabolism homeostasis. Here, to produce hFGF21 efficiently in Escherichia coli, the expression and solubility of hFGF21 were tested and optimised by fusing the protein with one of eight...

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Detalles Bibliográficos
Autores principales:	Nguyen, Anh Ngoc, Song, Jung-A, Nguyen, Minh Tan, Do, Bich Hang, Kwon, Grace G., Park, Sang Su, Yoo, Jiwon, Jang, Jaepyeong, Jin, Jonghwa, Osborn, Mark J., Jang, Yeon Jin, Thi Vu, Thu Trang, Oh, Heung-Bum, Choe, Han
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700921/ https://www.ncbi.nlm.nih.gov/pubmed/29170489 http://dx.doi.org/10.1038/s41598-017-16167-x

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