Cargando…

Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening

This study demonstrates the quantitative capabilities of coated blade spray (CBS) mass spectrometry (MS) for the concomitant analysis of multiple target substances in biofluid spots. In CBS-MS the analytes present in a given sample are first isolated and enriched in the thin coating of the CBS devic...

Descripción completa

Detalles Bibliográficos
Autores principales: Gómez-Ríos, Germán Augusto, Tascon, Marcos, Reyes-Garcés, Nathaly, Boyacı, Ezel, Poole, Justen, Pawliszyn, Janusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701014/
https://www.ncbi.nlm.nih.gov/pubmed/29170449
http://dx.doi.org/10.1038/s41598-017-16494-z
_version_ 1783281243267268608
author Gómez-Ríos, Germán Augusto
Tascon, Marcos
Reyes-Garcés, Nathaly
Boyacı, Ezel
Poole, Justen
Pawliszyn, Janusz
author_facet Gómez-Ríos, Germán Augusto
Tascon, Marcos
Reyes-Garcés, Nathaly
Boyacı, Ezel
Poole, Justen
Pawliszyn, Janusz
author_sort Gómez-Ríos, Germán Augusto
collection PubMed
description This study demonstrates the quantitative capabilities of coated blade spray (CBS) mass spectrometry (MS) for the concomitant analysis of multiple target substances in biofluid spots. In CBS-MS the analytes present in a given sample are first isolated and enriched in the thin coating of the CBS device. After a quick rinsing of the blade surface, as to remove remaining matrix, the analytes are quickly desorbed with the help of a solvent and then directly electrosprayed into the MS analyzer. Diverse pain management drugs, controlled substances, and therapeutic medications were successfully determined using only 10 µL of biofluid, with limits of quantitation in the low/sub ng·mL(−1) level attained within 7 minutes.
format Online
Article
Text
id pubmed-5701014
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-57010142017-11-30 Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening Gómez-Ríos, Germán Augusto Tascon, Marcos Reyes-Garcés, Nathaly Boyacı, Ezel Poole, Justen Pawliszyn, Janusz Sci Rep Article This study demonstrates the quantitative capabilities of coated blade spray (CBS) mass spectrometry (MS) for the concomitant analysis of multiple target substances in biofluid spots. In CBS-MS the analytes present in a given sample are first isolated and enriched in the thin coating of the CBS device. After a quick rinsing of the blade surface, as to remove remaining matrix, the analytes are quickly desorbed with the help of a solvent and then directly electrosprayed into the MS analyzer. Diverse pain management drugs, controlled substances, and therapeutic medications were successfully determined using only 10 µL of biofluid, with limits of quantitation in the low/sub ng·mL(−1) level attained within 7 minutes. Nature Publishing Group UK 2017-11-23 /pmc/articles/PMC5701014/ /pubmed/29170449 http://dx.doi.org/10.1038/s41598-017-16494-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gómez-Ríos, Germán Augusto
Tascon, Marcos
Reyes-Garcés, Nathaly
Boyacı, Ezel
Poole, Justen
Pawliszyn, Janusz
Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening
title Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening
title_full Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening
title_fullStr Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening
title_full_unstemmed Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening
title_short Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening
title_sort quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701014/
https://www.ncbi.nlm.nih.gov/pubmed/29170449
http://dx.doi.org/10.1038/s41598-017-16494-z
work_keys_str_mv AT gomezriosgermanaugusto quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening
AT tasconmarcos quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening
AT reyesgarcesnathaly quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening
AT boyacıezel quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening
AT poolejusten quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening
AT pawliszynjanusz quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening