Cargando…
Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening
This study demonstrates the quantitative capabilities of coated blade spray (CBS) mass spectrometry (MS) for the concomitant analysis of multiple target substances in biofluid spots. In CBS-MS the analytes present in a given sample are first isolated and enriched in the thin coating of the CBS devic...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701014/ https://www.ncbi.nlm.nih.gov/pubmed/29170449 http://dx.doi.org/10.1038/s41598-017-16494-z |
_version_ | 1783281243267268608 |
---|---|
author | Gómez-Ríos, Germán Augusto Tascon, Marcos Reyes-Garcés, Nathaly Boyacı, Ezel Poole, Justen Pawliszyn, Janusz |
author_facet | Gómez-Ríos, Germán Augusto Tascon, Marcos Reyes-Garcés, Nathaly Boyacı, Ezel Poole, Justen Pawliszyn, Janusz |
author_sort | Gómez-Ríos, Germán Augusto |
collection | PubMed |
description | This study demonstrates the quantitative capabilities of coated blade spray (CBS) mass spectrometry (MS) for the concomitant analysis of multiple target substances in biofluid spots. In CBS-MS the analytes present in a given sample are first isolated and enriched in the thin coating of the CBS device. After a quick rinsing of the blade surface, as to remove remaining matrix, the analytes are quickly desorbed with the help of a solvent and then directly electrosprayed into the MS analyzer. Diverse pain management drugs, controlled substances, and therapeutic medications were successfully determined using only 10 µL of biofluid, with limits of quantitation in the low/sub ng·mL(−1) level attained within 7 minutes. |
format | Online Article Text |
id | pubmed-5701014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57010142017-11-30 Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening Gómez-Ríos, Germán Augusto Tascon, Marcos Reyes-Garcés, Nathaly Boyacı, Ezel Poole, Justen Pawliszyn, Janusz Sci Rep Article This study demonstrates the quantitative capabilities of coated blade spray (CBS) mass spectrometry (MS) for the concomitant analysis of multiple target substances in biofluid spots. In CBS-MS the analytes present in a given sample are first isolated and enriched in the thin coating of the CBS device. After a quick rinsing of the blade surface, as to remove remaining matrix, the analytes are quickly desorbed with the help of a solvent and then directly electrosprayed into the MS analyzer. Diverse pain management drugs, controlled substances, and therapeutic medications were successfully determined using only 10 µL of biofluid, with limits of quantitation in the low/sub ng·mL(−1) level attained within 7 minutes. Nature Publishing Group UK 2017-11-23 /pmc/articles/PMC5701014/ /pubmed/29170449 http://dx.doi.org/10.1038/s41598-017-16494-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gómez-Ríos, Germán Augusto Tascon, Marcos Reyes-Garcés, Nathaly Boyacı, Ezel Poole, Justen Pawliszyn, Janusz Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening |
title | Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening |
title_full | Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening |
title_fullStr | Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening |
title_full_unstemmed | Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening |
title_short | Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening |
title_sort | quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701014/ https://www.ncbi.nlm.nih.gov/pubmed/29170449 http://dx.doi.org/10.1038/s41598-017-16494-z |
work_keys_str_mv | AT gomezriosgermanaugusto quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening AT tasconmarcos quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening AT reyesgarcesnathaly quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening AT boyacıezel quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening AT poolejusten quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening AT pawliszynjanusz quantitativeanalysisofbiofluidspotsbycoatedbladespraymassspectrometryanewapproachtorapidscreening |