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Quantitative analysis of motor evoked potentials in the neonatal lamb

Evoking motor potentials are an objective assessment method for neuromotor function, yet this was to our knowledge never done in neonatal lambs. There is neither a method for standardized quantification of motor evoked potentials (MEPs). We first aimed to evaluate the feasibility of MEP recording in...

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Autores principales: Joyeux, Luc, Deprez, Marjolijn, Khatoun, Ahmad, Van Kuyck, Kris, Pelsmaekers, Kelly, Engels, Alexander C., Wang, Hongmei, Monteiro Carvalho Mori da Cunha, Marina Gabriela, De Vleeschauwer, Stephanie, Mc Laughlin, Myles, Deprest, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701025/
https://www.ncbi.nlm.nih.gov/pubmed/29170524
http://dx.doi.org/10.1038/s41598-017-16453-8
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author Joyeux, Luc
Deprez, Marjolijn
Khatoun, Ahmad
Van Kuyck, Kris
Pelsmaekers, Kelly
Engels, Alexander C.
Wang, Hongmei
Monteiro Carvalho Mori da Cunha, Marina Gabriela
De Vleeschauwer, Stephanie
Mc Laughlin, Myles
Deprest, Jan
author_facet Joyeux, Luc
Deprez, Marjolijn
Khatoun, Ahmad
Van Kuyck, Kris
Pelsmaekers, Kelly
Engels, Alexander C.
Wang, Hongmei
Monteiro Carvalho Mori da Cunha, Marina Gabriela
De Vleeschauwer, Stephanie
Mc Laughlin, Myles
Deprest, Jan
author_sort Joyeux, Luc
collection PubMed
description Evoking motor potentials are an objective assessment method for neuromotor function, yet this was to our knowledge never done in neonatal lambs. There is neither a method for standardized quantification of motor evoked potentials (MEPs). We first aimed to evaluate the feasibility of MEP recording in neonatal lambs and test its validity. Second we aimed to develop an algorithm for its quantification and test its reliability since manual input is required. We recorded myogenic MEPs after transcranial motor cortex stimulation in 6 lambs aged 1–2 days. MEPs were also measured in one lamb undergoing Neuro-Muscular Blockade (NMB) and another undergoing lumbar spinal cord (SC) transection, both serving as controls. We computed 5 parameters using a custom-made algorithm: motor threshold, latency, area-under-the-curve, peak-to-peak amplitude and duration. Intra- and inter-observer reliability was analyzed. MEPs could be easily recorded, disappearing after NMB and SC transection. The algorithm allowed for analysis, hence physiologic readings of the parameters in all 4 limbs of all lambs were obtained. Our method was shown to have high intra- and inter-observer ( ≥70%) reliability for latency, area-under-the-curve and peak-to-peak amplitude. These results suggest that standardized MEP recording and analysis in neonatal lambs is feasible, and can reliably assess neuromotor function.
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spelling pubmed-57010252017-11-30 Quantitative analysis of motor evoked potentials in the neonatal lamb Joyeux, Luc Deprez, Marjolijn Khatoun, Ahmad Van Kuyck, Kris Pelsmaekers, Kelly Engels, Alexander C. Wang, Hongmei Monteiro Carvalho Mori da Cunha, Marina Gabriela De Vleeschauwer, Stephanie Mc Laughlin, Myles Deprest, Jan Sci Rep Article Evoking motor potentials are an objective assessment method for neuromotor function, yet this was to our knowledge never done in neonatal lambs. There is neither a method for standardized quantification of motor evoked potentials (MEPs). We first aimed to evaluate the feasibility of MEP recording in neonatal lambs and test its validity. Second we aimed to develop an algorithm for its quantification and test its reliability since manual input is required. We recorded myogenic MEPs after transcranial motor cortex stimulation in 6 lambs aged 1–2 days. MEPs were also measured in one lamb undergoing Neuro-Muscular Blockade (NMB) and another undergoing lumbar spinal cord (SC) transection, both serving as controls. We computed 5 parameters using a custom-made algorithm: motor threshold, latency, area-under-the-curve, peak-to-peak amplitude and duration. Intra- and inter-observer reliability was analyzed. MEPs could be easily recorded, disappearing after NMB and SC transection. The algorithm allowed for analysis, hence physiologic readings of the parameters in all 4 limbs of all lambs were obtained. Our method was shown to have high intra- and inter-observer ( ≥70%) reliability for latency, area-under-the-curve and peak-to-peak amplitude. These results suggest that standardized MEP recording and analysis in neonatal lambs is feasible, and can reliably assess neuromotor function. Nature Publishing Group UK 2017-11-23 /pmc/articles/PMC5701025/ /pubmed/29170524 http://dx.doi.org/10.1038/s41598-017-16453-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Joyeux, Luc
Deprez, Marjolijn
Khatoun, Ahmad
Van Kuyck, Kris
Pelsmaekers, Kelly
Engels, Alexander C.
Wang, Hongmei
Monteiro Carvalho Mori da Cunha, Marina Gabriela
De Vleeschauwer, Stephanie
Mc Laughlin, Myles
Deprest, Jan
Quantitative analysis of motor evoked potentials in the neonatal lamb
title Quantitative analysis of motor evoked potentials in the neonatal lamb
title_full Quantitative analysis of motor evoked potentials in the neonatal lamb
title_fullStr Quantitative analysis of motor evoked potentials in the neonatal lamb
title_full_unstemmed Quantitative analysis of motor evoked potentials in the neonatal lamb
title_short Quantitative analysis of motor evoked potentials in the neonatal lamb
title_sort quantitative analysis of motor evoked potentials in the neonatal lamb
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701025/
https://www.ncbi.nlm.nih.gov/pubmed/29170524
http://dx.doi.org/10.1038/s41598-017-16453-8
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