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An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells

Integration of chemical probes into proteomic workflows enables the interrogation of protein activity, rather than abundance. Current methods limit the biological contexts that can be addressed due to sample homogenization, signal-averaging, and bias toward abundant proteins. Here we report a platfo...

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Detalles Bibliográficos
Autores principales:	Li, Gang, Montgomery, Jeffrey E., Eckert, Mark A., Chang, Jae Won, Tienda, Samantha M., Lengyel, Ernst, Moellering, Raymond E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701173/ https://www.ncbi.nlm.nih.gov/pubmed/29176560 http://dx.doi.org/10.1038/s41467-017-01854-0

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