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Nanocarrier-mediated delivery of α-mangostin for non-surgical castration of male animals

The overpopulation of abandoned and stray companion animals has become a global crisis. The main purpose of this study was to develop a novel nanomedicine-based antifertility compound for non-surgical castration of male animals. Mangosteen (Garcinia mangostana L) pericarp extract has been shown to e...

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Autores principales: Yostawonkul, Jakarwan, Surassmo, Suvimol, Namdee, Katawut, Khongkow, Mattaka, Boonthum, Chatwalee, Pagseesing, Sasithon, Saengkrit, Nattika, Ruktanonchai, Uracha Rungsardthong, Chatdarong, Kaywalee, Ponglowhapan, Suppawiwat, Yata, Teerapong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701201/
https://www.ncbi.nlm.nih.gov/pubmed/29176590
http://dx.doi.org/10.1038/s41598-017-16563-3
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author Yostawonkul, Jakarwan
Surassmo, Suvimol
Namdee, Katawut
Khongkow, Mattaka
Boonthum, Chatwalee
Pagseesing, Sasithon
Saengkrit, Nattika
Ruktanonchai, Uracha Rungsardthong
Chatdarong, Kaywalee
Ponglowhapan, Suppawiwat
Yata, Teerapong
author_facet Yostawonkul, Jakarwan
Surassmo, Suvimol
Namdee, Katawut
Khongkow, Mattaka
Boonthum, Chatwalee
Pagseesing, Sasithon
Saengkrit, Nattika
Ruktanonchai, Uracha Rungsardthong
Chatdarong, Kaywalee
Ponglowhapan, Suppawiwat
Yata, Teerapong
author_sort Yostawonkul, Jakarwan
collection PubMed
description The overpopulation of abandoned and stray companion animals has become a global crisis. The main purpose of this study was to develop a novel nanomedicine-based antifertility compound for non-surgical castration of male animals. Mangosteen (Garcinia mangostana L) pericarp extract has been shown to exhibit anti-fertility property. α-mangostin (AM)-loaded nanostructured lipid carrier (AM-NLC) was developed to improve male germ cell apoptosis. This study was conducted to investigate physicochemical properties of AM-NLC and determine the biological effects of AM-NLC on spermatogonia cells and testicular explants obtained from castrated testes. AM-NLC was produced through a hot homogenization technique. The negatively charged particle of AM-NLC was nano-sized with a narrow dispersity. AM-NLC exhibited antiproliferative activity towards spermatogonium cells. It induced apoptosis in the cells. In addition, AM-NLC exhibited anti-inflammatory activities in lipopolysaccharide-activated macrophages. Abnormal anatomy of seminiferous tubule was noted following treatment of testicular explant with AM-NLC. This nanomedicine-based sterilant would be a promising platform that may have utility in non-surgical castration of male animals by intra-testicular injection.
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spelling pubmed-57012012017-11-30 Nanocarrier-mediated delivery of α-mangostin for non-surgical castration of male animals Yostawonkul, Jakarwan Surassmo, Suvimol Namdee, Katawut Khongkow, Mattaka Boonthum, Chatwalee Pagseesing, Sasithon Saengkrit, Nattika Ruktanonchai, Uracha Rungsardthong Chatdarong, Kaywalee Ponglowhapan, Suppawiwat Yata, Teerapong Sci Rep Article The overpopulation of abandoned and stray companion animals has become a global crisis. The main purpose of this study was to develop a novel nanomedicine-based antifertility compound for non-surgical castration of male animals. Mangosteen (Garcinia mangostana L) pericarp extract has been shown to exhibit anti-fertility property. α-mangostin (AM)-loaded nanostructured lipid carrier (AM-NLC) was developed to improve male germ cell apoptosis. This study was conducted to investigate physicochemical properties of AM-NLC and determine the biological effects of AM-NLC on spermatogonia cells and testicular explants obtained from castrated testes. AM-NLC was produced through a hot homogenization technique. The negatively charged particle of AM-NLC was nano-sized with a narrow dispersity. AM-NLC exhibited antiproliferative activity towards spermatogonium cells. It induced apoptosis in the cells. In addition, AM-NLC exhibited anti-inflammatory activities in lipopolysaccharide-activated macrophages. Abnormal anatomy of seminiferous tubule was noted following treatment of testicular explant with AM-NLC. This nanomedicine-based sterilant would be a promising platform that may have utility in non-surgical castration of male animals by intra-testicular injection. Nature Publishing Group UK 2017-11-24 /pmc/articles/PMC5701201/ /pubmed/29176590 http://dx.doi.org/10.1038/s41598-017-16563-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yostawonkul, Jakarwan
Surassmo, Suvimol
Namdee, Katawut
Khongkow, Mattaka
Boonthum, Chatwalee
Pagseesing, Sasithon
Saengkrit, Nattika
Ruktanonchai, Uracha Rungsardthong
Chatdarong, Kaywalee
Ponglowhapan, Suppawiwat
Yata, Teerapong
Nanocarrier-mediated delivery of α-mangostin for non-surgical castration of male animals
title Nanocarrier-mediated delivery of α-mangostin for non-surgical castration of male animals
title_full Nanocarrier-mediated delivery of α-mangostin for non-surgical castration of male animals
title_fullStr Nanocarrier-mediated delivery of α-mangostin for non-surgical castration of male animals
title_full_unstemmed Nanocarrier-mediated delivery of α-mangostin for non-surgical castration of male animals
title_short Nanocarrier-mediated delivery of α-mangostin for non-surgical castration of male animals
title_sort nanocarrier-mediated delivery of α-mangostin for non-surgical castration of male animals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701201/
https://www.ncbi.nlm.nih.gov/pubmed/29176590
http://dx.doi.org/10.1038/s41598-017-16563-3
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