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Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity

Green fluorescent protein (GFP) fusions are pervasively used to study structures and processes. Specific GFP-binders are thus of great utility for detection, immobilization or manipulation of GFP-fused molecules. We determined structures of two designed ankyrin repeat proteins (DARPins), complexed w...

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Detalles Bibliográficos
Autores principales: Hansen, Simon, Stüber, Jakob C., Ernst, Patrick, Koch, Alexander, Bojar, Daniel, Batyuk, Alexander, Plückthun, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701241/
https://www.ncbi.nlm.nih.gov/pubmed/29176615
http://dx.doi.org/10.1038/s41598-017-15711-z