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Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity
Green fluorescent protein (GFP) fusions are pervasively used to study structures and processes. Specific GFP-binders are thus of great utility for detection, immobilization or manipulation of GFP-fused molecules. We determined structures of two designed ankyrin repeat proteins (DARPins), complexed w...
Autores principales: | Hansen, Simon, Stüber, Jakob C., Ernst, Patrick, Koch, Alexander, Bojar, Daniel, Batyuk, Alexander, Plückthun, Andreas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701241/ https://www.ncbi.nlm.nih.gov/pubmed/29176615 http://dx.doi.org/10.1038/s41598-017-15711-z |
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