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Inter-species population dynamics enhance microbial horizontal gene transfer and spread of antibiotic resistance

Horizontal gene transfer (HGT) plays a major role in the spread of antibiotic resistance. Of particular concern are Acinetobacter baumannii bacteria, which recently emerged as global pathogens, with nosocomial mortality rates reaching 19–54% (Centers for Disease Control and Prevention, 2013; Joly Gu...

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Detalles Bibliográficos
Autores principales: Cooper, Robert M, Tsimring, Lev, Hasty, Jeff
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701796/
https://www.ncbi.nlm.nih.gov/pubmed/29091031
http://dx.doi.org/10.7554/eLife.25950
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author Cooper, Robert M
Tsimring, Lev
Hasty, Jeff
author_facet Cooper, Robert M
Tsimring, Lev
Hasty, Jeff
author_sort Cooper, Robert M
collection PubMed
description Horizontal gene transfer (HGT) plays a major role in the spread of antibiotic resistance. Of particular concern are Acinetobacter baumannii bacteria, which recently emerged as global pathogens, with nosocomial mortality rates reaching 19–54% (Centers for Disease Control and Prevention, 2013; Joly Guillou, 2005; Talbot et al., 2006). Acinetobacter gains antibiotic resistance remarkably rapidly (Antunes et al., 2014; Joly Guillou, 2005), with multi drug-resistance (MDR) rates exceeding 60% (Antunes et al., 2014; Centers for Disease Control and Prevention, 2013). Despite growing concern (Centers for Disease Control and Prevention, 2013; Talbot et al., 2006), the mechanisms underlying this extensive HGT remain poorly understood (Adams et al., 2008; Fournier et al., 2006; Imperi et al., 2011; Ramirez et al., 2010; Wilharm et al., 2013). Here, we show bacterial predation by Acinetobacter baylyi increases cross-species HGT by orders of magnitude, and we observe predator cells functionally acquiring adaptive resistance genes from adjacent prey. We then develop a population-dynamic model quantifying killing and HGT on solid surfaces. We show DNA released via cell lysis is readily available for HGT and may be partially protected from the environment, describe the effects of cell density, and evaluate potential environmental inhibitors. These findings establish a framework for understanding, quantifying, and combating HGT within the microbiome and the emergence of MDR super-bugs.
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spelling pubmed-57017962017-11-27 Inter-species population dynamics enhance microbial horizontal gene transfer and spread of antibiotic resistance Cooper, Robert M Tsimring, Lev Hasty, Jeff eLife Computational and Systems Biology Horizontal gene transfer (HGT) plays a major role in the spread of antibiotic resistance. Of particular concern are Acinetobacter baumannii bacteria, which recently emerged as global pathogens, with nosocomial mortality rates reaching 19–54% (Centers for Disease Control and Prevention, 2013; Joly Guillou, 2005; Talbot et al., 2006). Acinetobacter gains antibiotic resistance remarkably rapidly (Antunes et al., 2014; Joly Guillou, 2005), with multi drug-resistance (MDR) rates exceeding 60% (Antunes et al., 2014; Centers for Disease Control and Prevention, 2013). Despite growing concern (Centers for Disease Control and Prevention, 2013; Talbot et al., 2006), the mechanisms underlying this extensive HGT remain poorly understood (Adams et al., 2008; Fournier et al., 2006; Imperi et al., 2011; Ramirez et al., 2010; Wilharm et al., 2013). Here, we show bacterial predation by Acinetobacter baylyi increases cross-species HGT by orders of magnitude, and we observe predator cells functionally acquiring adaptive resistance genes from adjacent prey. We then develop a population-dynamic model quantifying killing and HGT on solid surfaces. We show DNA released via cell lysis is readily available for HGT and may be partially protected from the environment, describe the effects of cell density, and evaluate potential environmental inhibitors. These findings establish a framework for understanding, quantifying, and combating HGT within the microbiome and the emergence of MDR super-bugs. eLife Sciences Publications, Ltd 2017-11-01 /pmc/articles/PMC5701796/ /pubmed/29091031 http://dx.doi.org/10.7554/eLife.25950 Text en © 2017, Cooper et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Computational and Systems Biology
Cooper, Robert M
Tsimring, Lev
Hasty, Jeff
Inter-species population dynamics enhance microbial horizontal gene transfer and spread of antibiotic resistance
title Inter-species population dynamics enhance microbial horizontal gene transfer and spread of antibiotic resistance
title_full Inter-species population dynamics enhance microbial horizontal gene transfer and spread of antibiotic resistance
title_fullStr Inter-species population dynamics enhance microbial horizontal gene transfer and spread of antibiotic resistance
title_full_unstemmed Inter-species population dynamics enhance microbial horizontal gene transfer and spread of antibiotic resistance
title_short Inter-species population dynamics enhance microbial horizontal gene transfer and spread of antibiotic resistance
title_sort inter-species population dynamics enhance microbial horizontal gene transfer and spread of antibiotic resistance
topic Computational and Systems Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701796/
https://www.ncbi.nlm.nih.gov/pubmed/29091031
http://dx.doi.org/10.7554/eLife.25950
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