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Differential Signaling and Sugar Exchanges in Response to Avirulent Pathogen- and Symbiont-Derived Molecules in Tobacco Cells

Plants interact with microbes whose ultimate aim is to exploit plant carbohydrates for their reproduction. Plant–microbe interactions (PMIs) are classified according to the nature of their trophic exchanges: while mutualistic microbes trade nutrients with plants, pathogens unilaterally divert carboh...

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Autores principales: Pfister, Carole, Bourque, Stéphane, Chatagnier, Odile, Chiltz, Annick, Fromentin, Jérôme, Van Tuinen, Diederik, Wipf, Daniel, Leborgne-Castel, Nathalie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701941/
https://www.ncbi.nlm.nih.gov/pubmed/29209286
http://dx.doi.org/10.3389/fmicb.2017.02228
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author Pfister, Carole
Bourque, Stéphane
Chatagnier, Odile
Chiltz, Annick
Fromentin, Jérôme
Van Tuinen, Diederik
Wipf, Daniel
Leborgne-Castel, Nathalie
author_facet Pfister, Carole
Bourque, Stéphane
Chatagnier, Odile
Chiltz, Annick
Fromentin, Jérôme
Van Tuinen, Diederik
Wipf, Daniel
Leborgne-Castel, Nathalie
author_sort Pfister, Carole
collection PubMed
description Plants interact with microbes whose ultimate aim is to exploit plant carbohydrates for their reproduction. Plant–microbe interactions (PMIs) are classified according to the nature of their trophic exchanges: while mutualistic microbes trade nutrients with plants, pathogens unilaterally divert carbohydrates. The early responses following microbe recognition and the subsequent control of plant sugar distribution are still poorly understood. To further decipher PMI functionality, we used tobacco cells treated with microbial molecules mimicking pathogenic or mutualistic PMIs, namely cryptogein, a defense elicitor, and chitotetrasaccharide (CO4), which is secreted by mycorrhizal fungi. CO4 was perceived by tobacco cells and triggered widespread transient signaling components such as a sharp cytosolic Ca(2+) elevation, NtrbohD-dependent H(2)O(2) production, and MAP kinase activation. These CO4-induced events differed from those induced by cryptogein, i.e., sustained events leading to cell death. Furthermore, cryptogein treatment inhibited glucose and sucrose uptake but not fructose uptake, and promoted the expression of NtSUT and NtSWEET sugar transporters, whereas CO4 had no effect on sugar uptake and only a slight effect on NtSWEET2B expression. Our results suggest that microbial molecules induce different signaling responses that reflect microbial lifestyle and the subsequent outcome of the interaction.
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spelling pubmed-57019412017-12-05 Differential Signaling and Sugar Exchanges in Response to Avirulent Pathogen- and Symbiont-Derived Molecules in Tobacco Cells Pfister, Carole Bourque, Stéphane Chatagnier, Odile Chiltz, Annick Fromentin, Jérôme Van Tuinen, Diederik Wipf, Daniel Leborgne-Castel, Nathalie Front Microbiol Microbiology Plants interact with microbes whose ultimate aim is to exploit plant carbohydrates for their reproduction. Plant–microbe interactions (PMIs) are classified according to the nature of their trophic exchanges: while mutualistic microbes trade nutrients with plants, pathogens unilaterally divert carbohydrates. The early responses following microbe recognition and the subsequent control of plant sugar distribution are still poorly understood. To further decipher PMI functionality, we used tobacco cells treated with microbial molecules mimicking pathogenic or mutualistic PMIs, namely cryptogein, a defense elicitor, and chitotetrasaccharide (CO4), which is secreted by mycorrhizal fungi. CO4 was perceived by tobacco cells and triggered widespread transient signaling components such as a sharp cytosolic Ca(2+) elevation, NtrbohD-dependent H(2)O(2) production, and MAP kinase activation. These CO4-induced events differed from those induced by cryptogein, i.e., sustained events leading to cell death. Furthermore, cryptogein treatment inhibited glucose and sucrose uptake but not fructose uptake, and promoted the expression of NtSUT and NtSWEET sugar transporters, whereas CO4 had no effect on sugar uptake and only a slight effect on NtSWEET2B expression. Our results suggest that microbial molecules induce different signaling responses that reflect microbial lifestyle and the subsequent outcome of the interaction. Frontiers Media S.A. 2017-11-20 /pmc/articles/PMC5701941/ /pubmed/29209286 http://dx.doi.org/10.3389/fmicb.2017.02228 Text en Copyright © 2017 Pfister, Bourque, Chatagnier, Chiltz, Fromentin, Van Tuinen, Wipf and Leborgne-Castel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Pfister, Carole
Bourque, Stéphane
Chatagnier, Odile
Chiltz, Annick
Fromentin, Jérôme
Van Tuinen, Diederik
Wipf, Daniel
Leborgne-Castel, Nathalie
Differential Signaling and Sugar Exchanges in Response to Avirulent Pathogen- and Symbiont-Derived Molecules in Tobacco Cells
title Differential Signaling and Sugar Exchanges in Response to Avirulent Pathogen- and Symbiont-Derived Molecules in Tobacco Cells
title_full Differential Signaling and Sugar Exchanges in Response to Avirulent Pathogen- and Symbiont-Derived Molecules in Tobacco Cells
title_fullStr Differential Signaling and Sugar Exchanges in Response to Avirulent Pathogen- and Symbiont-Derived Molecules in Tobacco Cells
title_full_unstemmed Differential Signaling and Sugar Exchanges in Response to Avirulent Pathogen- and Symbiont-Derived Molecules in Tobacco Cells
title_short Differential Signaling and Sugar Exchanges in Response to Avirulent Pathogen- and Symbiont-Derived Molecules in Tobacco Cells
title_sort differential signaling and sugar exchanges in response to avirulent pathogen- and symbiont-derived molecules in tobacco cells
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701941/
https://www.ncbi.nlm.nih.gov/pubmed/29209286
http://dx.doi.org/10.3389/fmicb.2017.02228
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