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Genetic relatedness of Vibrio cholerae isolates within and between households during outbreaks in Dhaka, Bangladesh
BACKGROUND: Household contacts of cholera patients have a 100 times higher risk of developing a cholera infection than the general population. To compare the genetic relatedness of clinical and water source Vibrio cholerae isolates from cholera patients’ households across three outbreaks, we analyze...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702050/ https://www.ncbi.nlm.nih.gov/pubmed/29178823 http://dx.doi.org/10.1186/s12864-017-4254-9 |
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author | George, Christine Marie Rashid, Mahamud Almeida, Mathieu Saif-Ur-Rahman, K. M. Monira, Shirajum Bhuyian, Md. Sazzadul Islam Hasan, Khaled Mahmud, Toslim T. Li, Shan Brubaker, Jessica Perin, Jamie Rahman, Zillur Mustafiz, Munshi Sack, David A. Sack, R. Bradley Alam, Munirul Stine, O. Colin |
author_facet | George, Christine Marie Rashid, Mahamud Almeida, Mathieu Saif-Ur-Rahman, K. M. Monira, Shirajum Bhuyian, Md. Sazzadul Islam Hasan, Khaled Mahmud, Toslim T. Li, Shan Brubaker, Jessica Perin, Jamie Rahman, Zillur Mustafiz, Munshi Sack, David A. Sack, R. Bradley Alam, Munirul Stine, O. Colin |
author_sort | George, Christine Marie |
collection | PubMed |
description | BACKGROUND: Household contacts of cholera patients have a 100 times higher risk of developing a cholera infection than the general population. To compare the genetic relatedness of clinical and water source Vibrio cholerae isolates from cholera patients’ households across three outbreaks, we analyzed these isolates using whole-genome-sequencing (WGS) and multilocus variable-number tandem-repeat analysis (MLVA). RESULTS: The WGS analyses revealed that 80% of households had source water isolates that were more closely related to clinical isolates from the same household than to any other isolates. While in another 20% of households an isolate from a person was more closely related to clinical isolates from another household than to source water isolates from their own household. The mean pairwise differences in single nucleotide-variant (SNV) counts for isolates from the same household were significantly lower than those for different households (2.4 vs. 7.7 p < 0.0001), and isolates from the same outbreak had significantly fewer mean pairwise differences compared to isolates from different outbreaks (mean: 6.2 vs. 8.0, p < 0.0001). Based on MLVA in outbreak 1, we observed that the majority of households had clinical isolates with MLVA genotypes related to other clinical isolates and unrelated to water source isolates from the same household. While in outbreak 3, there were different MLVA genotypes between households, however within the majority of households, the clinical and water source isolates had the same MLVA genotypes. The beginning of outbreak 2 resembled outbreak 1 and the latter part resembled outbreak 3. We validated our use of MLVA by comparing it to WGS. Isolates with the identical MLVA genotype had significantly fewer mean pairwise SNV differences than those isolates with different MLVA genotypes (mean: 4.8 vs. 7.7, p < 0.0001). Furthermore, consistent with WGS results, the number of pairwise differences in the five MLVA loci for isolates within the same household was significantly lower than isolates from different households (mean: 1.6 vs. 3.0, p < 0.0001). CONCLUSION: These results suggest that transmission patterns for cholera are a combination of person-to-person and water-to-person cholera transmission with the proportions of the two modes varying within and between outbreaks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-017-4254-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5702050 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57020502017-12-04 Genetic relatedness of Vibrio cholerae isolates within and between households during outbreaks in Dhaka, Bangladesh George, Christine Marie Rashid, Mahamud Almeida, Mathieu Saif-Ur-Rahman, K. M. Monira, Shirajum Bhuyian, Md. Sazzadul Islam Hasan, Khaled Mahmud, Toslim T. Li, Shan Brubaker, Jessica Perin, Jamie Rahman, Zillur Mustafiz, Munshi Sack, David A. Sack, R. Bradley Alam, Munirul Stine, O. Colin BMC Genomics Research Article BACKGROUND: Household contacts of cholera patients have a 100 times higher risk of developing a cholera infection than the general population. To compare the genetic relatedness of clinical and water source Vibrio cholerae isolates from cholera patients’ households across three outbreaks, we analyzed these isolates using whole-genome-sequencing (WGS) and multilocus variable-number tandem-repeat analysis (MLVA). RESULTS: The WGS analyses revealed that 80% of households had source water isolates that were more closely related to clinical isolates from the same household than to any other isolates. While in another 20% of households an isolate from a person was more closely related to clinical isolates from another household than to source water isolates from their own household. The mean pairwise differences in single nucleotide-variant (SNV) counts for isolates from the same household were significantly lower than those for different households (2.4 vs. 7.7 p < 0.0001), and isolates from the same outbreak had significantly fewer mean pairwise differences compared to isolates from different outbreaks (mean: 6.2 vs. 8.0, p < 0.0001). Based on MLVA in outbreak 1, we observed that the majority of households had clinical isolates with MLVA genotypes related to other clinical isolates and unrelated to water source isolates from the same household. While in outbreak 3, there were different MLVA genotypes between households, however within the majority of households, the clinical and water source isolates had the same MLVA genotypes. The beginning of outbreak 2 resembled outbreak 1 and the latter part resembled outbreak 3. We validated our use of MLVA by comparing it to WGS. Isolates with the identical MLVA genotype had significantly fewer mean pairwise SNV differences than those isolates with different MLVA genotypes (mean: 4.8 vs. 7.7, p < 0.0001). Furthermore, consistent with WGS results, the number of pairwise differences in the five MLVA loci for isolates within the same household was significantly lower than isolates from different households (mean: 1.6 vs. 3.0, p < 0.0001). CONCLUSION: These results suggest that transmission patterns for cholera are a combination of person-to-person and water-to-person cholera transmission with the proportions of the two modes varying within and between outbreaks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-017-4254-9) contains supplementary material, which is available to authorized users. BioMed Central 2017-11-25 /pmc/articles/PMC5702050/ /pubmed/29178823 http://dx.doi.org/10.1186/s12864-017-4254-9 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article George, Christine Marie Rashid, Mahamud Almeida, Mathieu Saif-Ur-Rahman, K. M. Monira, Shirajum Bhuyian, Md. Sazzadul Islam Hasan, Khaled Mahmud, Toslim T. Li, Shan Brubaker, Jessica Perin, Jamie Rahman, Zillur Mustafiz, Munshi Sack, David A. Sack, R. Bradley Alam, Munirul Stine, O. Colin Genetic relatedness of Vibrio cholerae isolates within and between households during outbreaks in Dhaka, Bangladesh |
title | Genetic relatedness of Vibrio cholerae isolates within and between households during outbreaks in Dhaka, Bangladesh |
title_full | Genetic relatedness of Vibrio cholerae isolates within and between households during outbreaks in Dhaka, Bangladesh |
title_fullStr | Genetic relatedness of Vibrio cholerae isolates within and between households during outbreaks in Dhaka, Bangladesh |
title_full_unstemmed | Genetic relatedness of Vibrio cholerae isolates within and between households during outbreaks in Dhaka, Bangladesh |
title_short | Genetic relatedness of Vibrio cholerae isolates within and between households during outbreaks in Dhaka, Bangladesh |
title_sort | genetic relatedness of vibrio cholerae isolates within and between households during outbreaks in dhaka, bangladesh |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702050/ https://www.ncbi.nlm.nih.gov/pubmed/29178823 http://dx.doi.org/10.1186/s12864-017-4254-9 |
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