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Transcriptional precision and accuracy in development: from measurements to models and mechanisms
During development, genes are transcribed at specific times, locations and levels. In recent years, the emergence of quantitative tools has significantly advanced our ability to measure transcription with high spatiotemporal resolution in vivo. Here, we highlight recent studies that have used these...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702068/ https://www.ncbi.nlm.nih.gov/pubmed/29089359 http://dx.doi.org/10.1242/dev.146563 |
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author | Bentovim, Lital Harden, Timothy T. DePace, Angela H. |
author_facet | Bentovim, Lital Harden, Timothy T. DePace, Angela H. |
author_sort | Bentovim, Lital |
collection | PubMed |
description | During development, genes are transcribed at specific times, locations and levels. In recent years, the emergence of quantitative tools has significantly advanced our ability to measure transcription with high spatiotemporal resolution in vivo. Here, we highlight recent studies that have used these tools to characterize transcription during development, and discuss the mechanisms that contribute to the precision and accuracy of the timing, location and level of transcription. We attempt to disentangle the discrepancies in how physicists and biologists use the term ‘precision' to facilitate interactions using a common language. We also highlight selected examples in which the coupling of mathematical modeling with experimental approaches has provided important mechanistic insights, and call for a more expansive use of mathematical modeling to exploit the wealth of quantitative data and advance our understanding of animal transcription. |
format | Online Article Text |
id | pubmed-5702068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-57020682018-11-01 Transcriptional precision and accuracy in development: from measurements to models and mechanisms Bentovim, Lital Harden, Timothy T. DePace, Angela H. Development Review During development, genes are transcribed at specific times, locations and levels. In recent years, the emergence of quantitative tools has significantly advanced our ability to measure transcription with high spatiotemporal resolution in vivo. Here, we highlight recent studies that have used these tools to characterize transcription during development, and discuss the mechanisms that contribute to the precision and accuracy of the timing, location and level of transcription. We attempt to disentangle the discrepancies in how physicists and biologists use the term ‘precision' to facilitate interactions using a common language. We also highlight selected examples in which the coupling of mathematical modeling with experimental approaches has provided important mechanistic insights, and call for a more expansive use of mathematical modeling to exploit the wealth of quantitative data and advance our understanding of animal transcription. The Company of Biologists Ltd 2017-11-01 /pmc/articles/PMC5702068/ /pubmed/29089359 http://dx.doi.org/10.1242/dev.146563 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Review Bentovim, Lital Harden, Timothy T. DePace, Angela H. Transcriptional precision and accuracy in development: from measurements to models and mechanisms |
title | Transcriptional precision and accuracy in development: from measurements to models and mechanisms |
title_full | Transcriptional precision and accuracy in development: from measurements to models and mechanisms |
title_fullStr | Transcriptional precision and accuracy in development: from measurements to models and mechanisms |
title_full_unstemmed | Transcriptional precision and accuracy in development: from measurements to models and mechanisms |
title_short | Transcriptional precision and accuracy in development: from measurements to models and mechanisms |
title_sort | transcriptional precision and accuracy in development: from measurements to models and mechanisms |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702068/ https://www.ncbi.nlm.nih.gov/pubmed/29089359 http://dx.doi.org/10.1242/dev.146563 |
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