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Transcriptional precision and accuracy in development: from measurements to models and mechanisms

During development, genes are transcribed at specific times, locations and levels. In recent years, the emergence of quantitative tools has significantly advanced our ability to measure transcription with high spatiotemporal resolution in vivo. Here, we highlight recent studies that have used these...

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Detalles Bibliográficos
Autores principales: Bentovim, Lital, Harden, Timothy T., DePace, Angela H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702068/
https://www.ncbi.nlm.nih.gov/pubmed/29089359
http://dx.doi.org/10.1242/dev.146563
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author Bentovim, Lital
Harden, Timothy T.
DePace, Angela H.
author_facet Bentovim, Lital
Harden, Timothy T.
DePace, Angela H.
author_sort Bentovim, Lital
collection PubMed
description During development, genes are transcribed at specific times, locations and levels. In recent years, the emergence of quantitative tools has significantly advanced our ability to measure transcription with high spatiotemporal resolution in vivo. Here, we highlight recent studies that have used these tools to characterize transcription during development, and discuss the mechanisms that contribute to the precision and accuracy of the timing, location and level of transcription. We attempt to disentangle the discrepancies in how physicists and biologists use the term ‘precision' to facilitate interactions using a common language. We also highlight selected examples in which the coupling of mathematical modeling with experimental approaches has provided important mechanistic insights, and call for a more expansive use of mathematical modeling to exploit the wealth of quantitative data and advance our understanding of animal transcription.
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spelling pubmed-57020682018-11-01 Transcriptional precision and accuracy in development: from measurements to models and mechanisms Bentovim, Lital Harden, Timothy T. DePace, Angela H. Development Review During development, genes are transcribed at specific times, locations and levels. In recent years, the emergence of quantitative tools has significantly advanced our ability to measure transcription with high spatiotemporal resolution in vivo. Here, we highlight recent studies that have used these tools to characterize transcription during development, and discuss the mechanisms that contribute to the precision and accuracy of the timing, location and level of transcription. We attempt to disentangle the discrepancies in how physicists and biologists use the term ‘precision' to facilitate interactions using a common language. We also highlight selected examples in which the coupling of mathematical modeling with experimental approaches has provided important mechanistic insights, and call for a more expansive use of mathematical modeling to exploit the wealth of quantitative data and advance our understanding of animal transcription. The Company of Biologists Ltd 2017-11-01 /pmc/articles/PMC5702068/ /pubmed/29089359 http://dx.doi.org/10.1242/dev.146563 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Review
Bentovim, Lital
Harden, Timothy T.
DePace, Angela H.
Transcriptional precision and accuracy in development: from measurements to models and mechanisms
title Transcriptional precision and accuracy in development: from measurements to models and mechanisms
title_full Transcriptional precision and accuracy in development: from measurements to models and mechanisms
title_fullStr Transcriptional precision and accuracy in development: from measurements to models and mechanisms
title_full_unstemmed Transcriptional precision and accuracy in development: from measurements to models and mechanisms
title_short Transcriptional precision and accuracy in development: from measurements to models and mechanisms
title_sort transcriptional precision and accuracy in development: from measurements to models and mechanisms
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702068/
https://www.ncbi.nlm.nih.gov/pubmed/29089359
http://dx.doi.org/10.1242/dev.146563
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