Cargando…

Reference gene validation for gene expression normalization in canine osteosarcoma: a geNorm algorithm approach

BACKGROUND: Quantitative PCR (qPCR) is a common method for quantifying mRNA expression. Given the heterogeneity present in tumor tissues, it is crucial to normalize target mRNA expression data using appropriate reference genes that are stably expressed under a variety of pathological and experimenta...

Descripción completa

Detalles Bibliográficos
Autores principales:	Selvarajah, Gayathri Thevi, Bonestroo, Floor A. S., Timmermans Sprang, Elpetra P. M., Kirpensteijn, Jolle, Mol, Jan A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702123/ https://www.ncbi.nlm.nih.gov/pubmed/29178874 http://dx.doi.org/10.1186/s12917-017-1281-3

Ejemplares similares

Gene expression profiling of canine osteosarcoma reveals genes associated with short and long survival times
por: Selvarajah, Gayathri T, et al.
Publicado: (2009)

Prognostic factors in canine appendicular osteosarcoma – a meta-analysis
por: Boerman, Ilse, et al.
Publicado: (2012)

A simple method to assess group difference in RT-qPCR reference gene selection using GeNorm: The case of the placental sex
por: St-Pierre, Joey, et al.
Publicado: (2017)

P-cadherin mutations are associated with high basal Wnt activity and stemness in canine mammary tumor cell lines
por: Timmermans-Sprang, Elpetra, et al.
Publicado: (2019)

Correction: P-cadherin mutations are associated with high basal Wnt activity and stemness in canine mammary tumor cell lines
por: Timmermans-Sprang, Elpetra, et al.
Publicado: (2020)

High basal Wnt signaling is further induced by PI3K/mTor inhibition but sensitive to cSRC inhibition in mammary carcinoma cell lines with HER2/3 overexpression
por: Timmermans-Sprang, Elpetra P. M., et al.
Publicado: (2015)

Molecular Signaling of Progesterone, Growth Hormone, Wnt, and HER in Mammary Glands of Dogs, Rodents, and Humans: New Treatment Target Identification
por: Timmermans-Sprang, Elpetra P. M., et al.
Publicado: (2017)

Ligand-Independent Canonical Wnt Activity in Canine Mammary Tumor Cell Lines Associated with Aberrant LEF1 Expression
por: Gracanin, Ana, et al.
Publicado: (2014)

Utility of contrast-enhanced computed tomography in the evaluation of canine insulinoma location
por: Buishand, Floryne O., et al.
Publicado: (2018)

Circulating MicroRNAs as Non-invasive Biomarkers for Canine Cushing's Syndrome
por: Sanders, Karin, et al.
Publicado: (2021)

The effect of interleukin-2 on canine peripheral nerve sheath tumours after marginal surgical excision: a double-blind randomized study
por: Haagsman, Annika N, et al.
Publicado: (2013)

Zerumbone-Loaded Nanostructured Lipid Carrier Induces Apoptosis of Canine Mammary Adenocarcinoma Cells
por: Foong, Jia Ning, et al.
Publicado: (2018)

Whole transcriptome analysis of canine pheochromocytoma and paraganglioma
por: van den Berg, Marit F., et al.
Publicado: (2023)

Pulmonary papillary adenocarcinoma with Aspergillus versicolor infection in a dog
por: Maniam, Rathiymaler, et al.
Publicado: (2017)

In Vitro Growth Inhibition, Caspase-Dependent Apoptosis, and S and G2/M Phase Arrest in Breast Cancer Cells Induced by Fluorine-Incorporated Gold I Compound, Ph3PAu[SC(OMe)=NC6H4F-3]
por: Yu, Richard Ming Chuan, et al.
Publicado: (2022)

Expression of Toll-like receptors 3, 7, 9 and cytokines in feline infectious peritonitis virus-infected CRFK cells and feline peripheral monocytes
por: Megat Mazhar Khair, Megat Hamzah, et al.
Publicado: (2022)

Toxicity and Safety Evaluation of Doxorubicin-Loaded Cockleshell-Derived Calcium Carbonate Nanoparticle in Dogs
por: Danmaigoro, Abubakar, et al.
Publicado: (2018)

Cellular Metabolic Profiling of CrFK Cells Infected with Feline Infectious Peritonitis Virus Using Phenotype Microarrays
por: Ng, Shing Wei, et al.
Publicado: (2020)

Addendum: Ng, S.W. et al. Cellular Metabolic Profiling of CrFK Cells Infected with Feline Infectious Peritonitis Virus Using Phenotype Microarrays. Pathogens 2020, 9, 412
por: Ng, Shing Wei, et al.
Publicado: (2020)

In Vitro Evaluation of Curcumin-Encapsulated Chitosan Nanoparticles against Feline Infectious Peritonitis Virus and Pharmacokinetics Study in Cats
por: Ng, Shing Wei, et al.
Publicado: (2020)

NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
por: Zakaria, Muhammad Asyaari, et al.
Publicado: (2021)

A laparoscopic approach for removal of ovarian remnant tissue in 32 dogs
por: van Nimwegen, Sebastiaan A., et al.
Publicado: (2018)

Expression profiles of immune mediators in feline Coronavirus-infected cells and clinical samples of feline Coronavirus-positive cats
por: Safi, Nikoo, et al.
Publicado: (2017)

Case Report: Radioactive Holmium-166 Microspheres for the Intratumoral Treatment of a Canine Pituitary Tumor
por: Morsink, Nino Chiron, et al.
Publicado: (2021)

Comparative aspects of microRNA expression in canine and human cancers
por: Sahabi, Kabiru, et al.
Publicado: (2018)

Transcriptomic analysis by RNA sequencing characterises malignant progression of canine insulinoma from normal tissue to metastatic disease
por: Capodanno, Y., et al.
Publicado: (2020)

Development of erythropoietin receptor-targeted drug delivery system against breast cancer using tamoxifen-loaded nanostructured lipid carriers
por: Beh, Chaw Yee, et al.
Publicado: (2017)

C-reactive protein, glucose and iron concentrations are significantly altered in dogs undergoing open ovariohysterectomy or ovariectomy
por: Moldal, Elena Regine, et al.
Publicado: (2018)

Intra- and interspecies gene expression models for predicting drug response in canine osteosarcoma
por: Fowles, Jared S., et al.
Publicado: (2016)

Postbronchodilator Reference Values: Should They Be the Norm?
por: Smith, Lewis J.
Publicado: (2023)

The Role of Breast Cancer Stem Cell-Related Biomarkers as Prognostic Factors
por: Ko, Clarence Ching Huat, et al.
Publicado: (2020)

A Comparison Study of the Tehran Norms to the Reference Norms on Children Performance of the Bayley III
por: SOLEIMANI, Farin, et al.
Publicado: (2022)

Reference genes for reverse transcription quantitative PCR in canine brain tissue
por: Stassen, Quirine E. M., et al.
Publicado: (2015)

Expression of Nociceptive Ligands in Canine Osteosarcoma
por: Shor, S., et al.
Publicado: (2015)

Expression of Bcl-2 in canine osteosarcoma
por: Piro, F., et al.
Publicado: (2015)

Molecular Mechanisms of Canine Osteosarcoma Metastasis
por: Wilk, Sylwia S., et al.
Publicado: (2021)

Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line
por: Pardo-Mora, Dolly Patricia, et al.
Publicado: (2021)

Transcriptional profiling of canine osteosarcoma identifies prognostic gene expression signatures with translational value for humans
por: Mannheimer, Joshua D., et al.
Publicado: (2023)

Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats
por: Harun, Mohammad Syamsul Reza, et al.
Publicado: (2013)

Nuclear DNA-Content in Mesenchymal Lesions in Dogs: Its Value as Marker of Malignancy and Extent of Genomic Instability
por: Boerkamp, Kim M., et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_ba303akfp76v858jivbknfeghg