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Comprehensive Analysis of Cytomegalovirus pp65 Antigen-Specific CD8(+) T Cell Responses According to Human Leukocyte Antigen Class I Allotypes and Intraindividual Dominance
To define whether individual human leukocyte antigen (HLA) class I allotypes are used preferentially in human cytomegalovirus (CMV)-specific cytotoxic T lymphocyte responses, CD8(+) T cell responses restricted by up to six HLA class I allotypes in an individual were measured in parallel using K562-b...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702484/ https://www.ncbi.nlm.nih.gov/pubmed/29209324 http://dx.doi.org/10.3389/fimmu.2017.01591 |
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author | Hyun, Seung-Joo Sohn, Hyun-Jung Lee, Hyun-Joo Lee, Seon-Duk Kim, Sueon Sohn, Dae-Hee Hong, Cheol-Hwa Choi, Haeyoun Cho, Hyun-Il Kim, Tai-Gyu |
author_facet | Hyun, Seung-Joo Sohn, Hyun-Jung Lee, Hyun-Joo Lee, Seon-Duk Kim, Sueon Sohn, Dae-Hee Hong, Cheol-Hwa Choi, Haeyoun Cho, Hyun-Il Kim, Tai-Gyu |
author_sort | Hyun, Seung-Joo |
collection | PubMed |
description | To define whether individual human leukocyte antigen (HLA) class I allotypes are used preferentially in human cytomegalovirus (CMV)-specific cytotoxic T lymphocyte responses, CD8(+) T cell responses restricted by up to six HLA class I allotypes in an individual were measured in parallel using K562-based artificial antigen-presenting cells expressing both CMV pp65 antigen and one of 32 HLA class I allotypes (7 HLA-A, 14 HLA-B, and 11 HLA-C) present in 50 healthy Korean donors. The CD8(+) T cell responses to pp65 in the HLA-C allotypes were lower than responses to those in HLA-A and -B allotypes and there was no difference between the HLA-A and HLA-B loci. HLA-A*02:01, -B*07:02, and -C*08:01 showed the highest magnitude and frequency of immune responses to pp65 at each HLA class I locus. However, HLA-A*02:07, -B*59:01, -B*58:01, -B*15:11, -C*03:02, and -C*02:02 did not show any immune responses. Although each individual has up to six different HLA allotypes, 46% of the donors showed one allotype, 24% showed two allotypes, and 2% showed three allotypes that responded to pp65. Interestingly, the frequencies of HLA-A alleles were significantly correlated with the positivity of specific allotypes. Our results demonstrate that specific HLA class I allotypes are preferentially used in the CD8(+) T cell immune response to pp65 and that a hierarchy among HLA class I allotypes is present in an individual. |
format | Online Article Text |
id | pubmed-5702484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57024842017-12-05 Comprehensive Analysis of Cytomegalovirus pp65 Antigen-Specific CD8(+) T Cell Responses According to Human Leukocyte Antigen Class I Allotypes and Intraindividual Dominance Hyun, Seung-Joo Sohn, Hyun-Jung Lee, Hyun-Joo Lee, Seon-Duk Kim, Sueon Sohn, Dae-Hee Hong, Cheol-Hwa Choi, Haeyoun Cho, Hyun-Il Kim, Tai-Gyu Front Immunol Immunology To define whether individual human leukocyte antigen (HLA) class I allotypes are used preferentially in human cytomegalovirus (CMV)-specific cytotoxic T lymphocyte responses, CD8(+) T cell responses restricted by up to six HLA class I allotypes in an individual were measured in parallel using K562-based artificial antigen-presenting cells expressing both CMV pp65 antigen and one of 32 HLA class I allotypes (7 HLA-A, 14 HLA-B, and 11 HLA-C) present in 50 healthy Korean donors. The CD8(+) T cell responses to pp65 in the HLA-C allotypes were lower than responses to those in HLA-A and -B allotypes and there was no difference between the HLA-A and HLA-B loci. HLA-A*02:01, -B*07:02, and -C*08:01 showed the highest magnitude and frequency of immune responses to pp65 at each HLA class I locus. However, HLA-A*02:07, -B*59:01, -B*58:01, -B*15:11, -C*03:02, and -C*02:02 did not show any immune responses. Although each individual has up to six different HLA allotypes, 46% of the donors showed one allotype, 24% showed two allotypes, and 2% showed three allotypes that responded to pp65. Interestingly, the frequencies of HLA-A alleles were significantly correlated with the positivity of specific allotypes. Our results demonstrate that specific HLA class I allotypes are preferentially used in the CD8(+) T cell immune response to pp65 and that a hierarchy among HLA class I allotypes is present in an individual. Frontiers Media S.A. 2017-11-21 /pmc/articles/PMC5702484/ /pubmed/29209324 http://dx.doi.org/10.3389/fimmu.2017.01591 Text en Copyright © 2017 Hyun, Sohn, Lee, Lee, Kim, Sohn, Hong, Choi, Cho and Kim. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Hyun, Seung-Joo Sohn, Hyun-Jung Lee, Hyun-Joo Lee, Seon-Duk Kim, Sueon Sohn, Dae-Hee Hong, Cheol-Hwa Choi, Haeyoun Cho, Hyun-Il Kim, Tai-Gyu Comprehensive Analysis of Cytomegalovirus pp65 Antigen-Specific CD8(+) T Cell Responses According to Human Leukocyte Antigen Class I Allotypes and Intraindividual Dominance |
title | Comprehensive Analysis of Cytomegalovirus pp65 Antigen-Specific CD8(+) T Cell Responses According to Human Leukocyte Antigen Class I Allotypes and Intraindividual Dominance |
title_full | Comprehensive Analysis of Cytomegalovirus pp65 Antigen-Specific CD8(+) T Cell Responses According to Human Leukocyte Antigen Class I Allotypes and Intraindividual Dominance |
title_fullStr | Comprehensive Analysis of Cytomegalovirus pp65 Antigen-Specific CD8(+) T Cell Responses According to Human Leukocyte Antigen Class I Allotypes and Intraindividual Dominance |
title_full_unstemmed | Comprehensive Analysis of Cytomegalovirus pp65 Antigen-Specific CD8(+) T Cell Responses According to Human Leukocyte Antigen Class I Allotypes and Intraindividual Dominance |
title_short | Comprehensive Analysis of Cytomegalovirus pp65 Antigen-Specific CD8(+) T Cell Responses According to Human Leukocyte Antigen Class I Allotypes and Intraindividual Dominance |
title_sort | comprehensive analysis of cytomegalovirus pp65 antigen-specific cd8(+) t cell responses according to human leukocyte antigen class i allotypes and intraindividual dominance |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702484/ https://www.ncbi.nlm.nih.gov/pubmed/29209324 http://dx.doi.org/10.3389/fimmu.2017.01591 |
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